Team:SUSTC-Shenzhen/Notebook/HeLa Cell/blasticidin screening

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Revision as of 23:03, 11 October 2014

Team SUSTC-Shenzhen

Notebook

Element of an endeavor


HeLa Cell

2014 --to test the restriction of hela cell to blasticidin

2014.8.8, 10:00

Material

Blasticidin, Hela cell, 24-holes plate

Results

Experimental group:

Row 1(0.5~1.6 ug/ml) still have many cells attach to the bottom

Row 2,3(1.8~4ug/ml) can clearly see some cells fall off, and mostly are single cell

Row 4(5~9ug/ml) certainly the cells fall off multiply

control group(0ug/ml):

there are few fallen cells, it should be normal

Repeat group:

Just see several cells become orbicular, and the fallen cells in all holes are less than experimental groups

Description

The cells grew asymmetric, more concentrated in the center but rarer in the surrounding, and the gradients are too dense to watch out for. We infer we can get the result after 2~3days

Discussion

There is situation that we add the antibiotics asymmetric, and we used a wrong way to mix up the cell with the solid medium


2014.8.8, 4:00~5:20

Results

Experimental group

8.0ug/ml 5% cells adherence almost cells float 0.5ug/ml 90% adherence several float 1.0ug/ml same as left same as left 1.2ug/ml same as left same as left 1.4ug/ml same as left floated cells increase 1.6ug/ml same as left same as left
1.8ug/ml 60%~70% adherence 2.0ug/ml same as left 2.2ug/ml 50%~60% adherence 2.4ug/ml 40%~50% adherence 2.6ug/ml same as left 2.8ug/ml same as left
3.0ug/ml 50%~60% adherence(increase) 3.2ug/ml same as left 3.4ug/ml same as left 3.6ug/ml same as left 3.8ug/ml same as left 4.0ug/ml same as left
5.0ug/ml adherence(decrease) float cell(increase) 6.0ug/ml same as left 7.0ug/ml same as left null null null

Repeat group

Totally, the tendency is the same as the experimental group, but there are some micro differences, the percentage of adherence is higher, meanwhile, the float cells are less than another group.

Description

The result is not the same as yesterday, and the spreading of cells is not the same spreading of cells

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