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Team:INSA-Lyon/notebook
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<h5>June </h5> | <h5>June </h5> | ||
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<h6>11/06 </h6> | <h6>11/06 </h6> | ||
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<p> Reception of the four DNA constructions: CsgA Wild type, CsgA PolyHis1, CsgA PolyHis2, CsgA Modular</p> | <p> Reception of the four DNA constructions: CsgA Wild type, CsgA PolyHis1, CsgA PolyHis2, CsgA Modular</p> | ||
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| - | <h6> | + | <h6>24/06 </h6> |
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| + | <p> NM522 and DH5α cells (in liquid LB medium) were cultured overnight under agitation at 37°C for later transformation. </p> | ||
| + | <br/> | ||
| + | <h6>25/06 </h6> | ||
| + | <br/> | ||
| + | <p> </p> | ||
| + | <br/> | ||
| + | <h6>25/06 </h6> | ||
| + | <br/> | ||
| + | <p> </p> | ||
| + | <br/> | ||
| + | <h6>25/06 </h6> | ||
| + | <br/> | ||
| + | <p>Transformation of NM522 and DH5α strains with each one of the following plasmids. </p> | ||
| + | <p>Selection of transformed bacteria on antibiotic plates depending on the plasmid. </p> | ||
| + | <br/> | ||
| + | <h6>26/06 </h6> | ||
| + | <br/> | ||
| + | <p>Transformation analysis: all the transformations were successful except the ones with plasmids pHC03, pHC06, pHC07 and pHC09. Transformation of these four plasmids was repeated with a new protocol. </p> | ||
| + | <p> Clones of transformed strains containing pHC01, 02, 04, 05 and 08 were cultured in 5 mL LB medium containing 50 µL of the specific antibiotic at 37°C and appropriate plates were streaked with isolated colonies likely to contain transformed bacteria. </p> | ||
| + | <br/><h6>27/06 </h6> | ||
| + | <br/> | ||
| + | <p>Transformation analysis: all the transformations were successful and plates containing pHC06 plasmids showed pink bacteria, as the rfp in the plasmid is expressed in presence of a strong promoter (like J23100). </p> | ||
| + | <p>A protocol for nickel titration was searched and nickel was stored for later experiments. </p> | ||
| + | <br/><h6>25/06 </h6> | ||
| + | <br/> | ||
| + | <p> </p> | ||
| + | <br/> | ||
Revision as of 22:43, 10 October 2014
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PROJECT
PROJECT- PROJECT SUMMARY
- ACHIEVEMENTS
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WETLAB
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MODELING
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HUMAN PRACTICE
HUMAN
PRACTICE- HUMAN PRACTICE SUMMARY
- SYNBIO PERCEPTION
- PROJECT'S INTEREST
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TEAM
NOTEBOOK
February-June
Literature searches were performed about Peptide Display, Curli biogenesis and metal trapping.
June
11/06
Reception of the four DNA constructions: CsgA Wild type, CsgA PolyHis1, CsgA PolyHis2, CsgA Modular
24/06
NM522 and DH5α cells (in liquid LB medium) were cultured overnight under agitation at 37°C for later transformation.
25/06
25/06
25/06
Transformation of NM522 and DH5α strains with each one of the following plasmids.
Selection of transformed bacteria on antibiotic plates depending on the plasmid.
26/06
Transformation analysis: all the transformations were successful except the ones with plasmids pHC03, pHC06, pHC07 and pHC09. Transformation of these four plasmids was repeated with a new protocol.
Clones of transformed strains containing pHC01, 02, 04, 05 and 08 were cultured in 5 mL LB medium containing 50 µL of the specific antibiotic at 37°C and appropriate plates were streaked with isolated colonies likely to contain transformed bacteria.
27/06
Transformation analysis: all the transformations were successful and plates containing pHC06 plasmids showed pink bacteria, as the rfp in the plasmid is expressed in presence of a strong promoter (like J23100).
A protocol for nickel titration was searched and nickel was stored for later experiments.
25/06
