Team:Austin Texas/kit
From 2014.igem.org
Line 116: | Line 116: | ||
| 181.19 | | 181.19 | ||
| 343 °C | | 343 °C | ||
- | | Soluble in water; heat to 70C and vortex to dissolve completely; Must stay | + | | Soluble in water; heat to 70C and vortex to dissolve completely; Must stay warm to remain in solution |
| Stock Concentration: 10mM; Concentration in Culture: 1mM | | Stock Concentration: 10mM; Concentration in Culture: 1mM | ||
Line 158: | Line 158: | ||
| 316.308 | | 316.308 | ||
| ? | | ? | ||
- | | Soluble in 50% DMSO in Water; Heat up to 70C and add | + | | Soluble in 50% DMSO in Water; Heat up to 70C and add NaOH to dissolve |
| Stock Concentration: 50mM; Concentration in Culture: 1mM; Light Sensitive | | Stock Concentration: 50mM; Concentration in Culture: 1mM; Light Sensitive | ||
Line 186: | Line 186: | ||
! Parent Synthetase | ! Parent Synthetase | ||
! Synthetase Mutations | ! Synthetase Mutations | ||
- | ! | + | ! tRNA |
! Synthetase Reference | ! Synthetase Reference | ||
! Sequence confirmed? | ! Sequence confirmed? | ||
|- | |- | ||
- | | | + | | Tyrosine RS |
| <i>M.jannaschii</i> Tyrosine aaRS (Mj-TyrRS) | | <i>M.jannaschii</i> Tyrosine aaRS (Mj-TyrRS) | ||
+ | | Wild Type | ||
+ | | Mj-tRNA Tyr (CUA) | ||
+ | | | ||
+ | | | ||
+ | |||
+ | |- | ||
+ | | Cyanophenylalanine RS | ||
+ | | (Mj-TyrRS) | ||
| Y32L, L65W, F108W, Q109M, D158G, I159A | | Y32L, L65W, F108W, Q109M, D158G, I159A | ||
| Mj-tRNA Tyr (CUA) | | Mj-tRNA Tyr (CUA) |
Revision as of 21:57, 10 October 2014
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