Team:Aachen/Notebook/Wetlab2

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       Organization and preparation
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Revision as of 10:16, 10 October 2014

Our work in the lab

April

1st

Organization

where to get:

  • key cards and keys for the lab
  • cooled centifuge
  • space in -80 °C
  • bottles for 70 % ethanol
  • ... (problems of a first year team ^^)

Read more about April here...

May

1st

  • gel with M - full -full - REACh1 SOE3.2 - REACH2 SOE3.2 - M
    → 120 V, 30 min
    → cut out the bands

Read more about May here...

June

3rd

  • transformation of 34 BioBricks

4th

  • preparation of consumables:
    • fresh 50 % glycerol
    • new LB plates with cam and with kanamycin (kan)
    • 60 glas tubes
    • 2 L LB-
    • 100 mL steril glas beads for plating
  • master plates (6 clones per BioBrick) were made

Read more about June here...

July

1st

  • transformation efficiency kit is broken

→ run an agarose gel with the kit plasmids to check for nuclease activity/ bands

  • inventory of the -80°C freezer was done

2nd

  • overnight cultures (LB) of K731520 and K1319042 for chips were inoculated

Read more about July here...

August

1st

  • made electrocompetent E.coli rosetta cells.
  • prepared cultures of K1319042 and K131026

2nd

  • tested the OD measurement device and compared it to the spectrophotometer and the plate reader.
  • tested K131026 and K1319042 for fluorescence in the plate reader
  • did a heat shock transformation of I746909 into NEB TOP 10 cells
  • did an electroshock transformation of pET17-Gal3 into E.coli rosetta

Read more about August here...

September

1st

  • 5 ml cultures of K1319003 and K1319004
  • plasmid prep
Plasmid DNA [ng/µl]
J23101.K516032 pSB1K3 23.5
J23115.K516032 pSB1K3 20.5
J04450 pSB1A3 57.5
J04450 pSB1K1 63.5
  • over night cultures of K131026 in DH5α and NEB

Read more about September here...

October

1st

  • Preparations for sensor-chip production on the following day (2014-10-02) was done accoringly to the sensor chip manufacturing protocol:
    • At 18:30 we prepared over-night cultures from K1319042, B0015 and K131026 by inoculating 250 ml Erlenmeyer flasks each containing 50 ml LB medium . The flasks were incubated for ~12 hours at 37 °C on a shaker.

Read more about October here...


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