Team:Penn State/Notebook

From 2014.igem.org

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   <td><center><b>Monday, May 25, 2014</b></center></td>
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   <td><center><b>Sunday, May 25, 2014</b></center></td>
   <td>Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the <i>P. putida</i> genome using homologous recombination. Ordered </td>
   <td>Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the <i>P. putida</i> genome using homologous recombination. Ordered </td>
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   <td><center><b>Tuesday, May 26, 2014</b></center></td>
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   <td><center><b>Monday, May 26, 2014</b></center></td>
   <td>Inoculated LB broth with ampicillin and dCas9 plasmid from cryogenic storage; inoculated Lb broth with chloramphenicol and FTV vector from Ashlee's past experiment; streaked the HMF vector on a kanamycin plate</td>
   <td>Inoculated LB broth with ampicillin and dCas9 plasmid from cryogenic storage; inoculated Lb broth with chloramphenicol and FTV vector from Ashlee's past experiment; streaked the HMF vector on a kanamycin plate</td>
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   <td><center><b>Wednesday, May 27, 2014</b></center></td>
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   <td><center><b>Tuesday, May 27, 2014</b></center></td>
   <td>Emily and Ashlee made cryogenic storage of the dCas9 plasmid; plasmid prepared the FTV and dCas9 vectors; digested FTV vector; inoculated LB broth with a colony from the HMF plate for overnight growth and plasmid preparation tomorrow</td>  
   <td>Emily and Ashlee made cryogenic storage of the dCas9 plasmid; plasmid prepared the FTV and dCas9 vectors; digested FTV vector; inoculated LB broth with a colony from the HMF plate for overnight growth and plasmid preparation tomorrow</td>  
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   <td><center><b>Thursday, May 28, 2014</b></center></td>
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   <td><center><b>Wednesday, May 28, 2014</b></center></td>
   <td>Prepared plasmid containing the HMF pathway; inoculated LB broth with Lambda Red Recombinase plasmid from cryogenic storage</td>
   <td>Prepared plasmid containing the HMF pathway; inoculated LB broth with Lambda Red Recombinase plasmid from cryogenic storage</td>
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   <td><center><b>Friday, May 29, 2014</b></center></td>
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   <td><center><b>Thursday, May 29, 2014</b></center></td>
   <td>Ashlee and Emily made cryogenic storage of the Lamda Red Recombinase plasmid</td>
   <td>Ashlee and Emily made cryogenic storage of the Lamda Red Recombinase plasmid</td>
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   <td><center><b>Saturday, May 30, 2014</b></center></td>
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   <td><center><b>Friday, May 30, 2014</b></center></td>
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   <td><center><b>Sunday, May 31, 2014</b></center></td>
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   <td><center><b>Saturday, May 31, 2014</b></center></td>
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   <td><center><b>Monday, June 1, 2014</b></center></td>
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   <td><center><b>Sunday, June 1, 2014</b></center></td>
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   <td><center><b>Monday, June 2, 2014</b></center></td>
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   <td><center><b>Tuesday, June 3, 2014</b></center></td>
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   <td><center><b>Wednesday, June 4, 2014</b></center></td>
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   <td><center><b>Thursday, June 5, 2014</b></center></td>
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   <td><center><b>Friday, June 6, 2014</b></center></td>
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   <td><center><b>Saturday, June 7, 2014</b></center></td>
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   <td><center><b>Sunday, June 8, 2014</b></center></td>
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   <td><center><b>Monday, June 9, 2014</b></center></td>
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   <td><center><b>Tuesday, June 10, 2014</b></center></td>
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   <td><center><b>Wednesday, June 11, 2014</b></center></td>
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   <td><center><b>Thursday, June 12, 2014</b></center></td>
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   <td><center><b>Friday, June 13, 2014</b></center></td>
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   <td><center><b>Saturday, June 14, 2014</b></center></td>
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   <td><center><b>Sunday June 15, 2014</b></center></td>
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   <td><center><b>Monday, June 16, 2014</b></center></td>
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   <td><center><b>Tuesday, June 17, 2014</b></center></td>
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   <td><center><b>Wednesday, June 18, 2014</b></center></td>
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   <td><center><b>Thursday, June 19, 2014</b></center></td>
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   <td><center><b>Friday, June 20, 2014</b></center></td>
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   <td><center><b>Saturday, June 21, 2014</b></center></td>
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   <td><center><b>Sunday, June 22, 2014</b></center></td>
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   <td><center><b>Monday, June 23, 2014</b></center></td>
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Revision as of 22:12, 19 June 2014


WELCOME TO iGEM 2014!

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On this page you can document your project, introduce your team members, document your progress
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Home Team Official Team Profile Project Parts Modeling Notebook Safety Human Practices Attributions

Penn State iGEM 2014 Notebook Page

Here you will find weekly summaries of our wet laboratory progress, team updates, and accomplishments outside the laboratory. Below [link this] is our detailed, day-to-day progress laboratory notebook.

Weekly Summaries

will create links to each week, but will just list down the page Week 1, Week 2, etc etc

Laboratory Notebook

Biodetoxification
Codon Optimization
Wednesday, May 21, 2014
 Emily's first experience with cloning! Ashlee led Emily through several practice experiments from designs made earlier in the year: making a gel, loading samples, gel purifying DNA ...................
Thursday, May 22, 2014
 Ashlee and Emily performed a transformation
Friday, May 23, 2014
 We picked several colonies for overnight growth to do more cloning tomorrow
Saturday, May 24, 2014
 Memorial Day Weekend? How about lab cloning weekend! Ashlee conducted plasmid preparation and digestion
Sunday, May 25, 2014
 Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the P. putida genome using homologous recombination. Ordered
Monday, May 26, 2014
 Inoculated LB broth with ampicillin and dCas9 plasmid from cryogenic storage; inoculated Lb broth with chloramphenicol and FTV vector from Ashlee's past experiment; streaked the HMF vector on a kanamycin plate
Tuesday, May 27, 2014
 Emily and Ashlee made cryogenic storage of the dCas9 plasmid; plasmid prepared the FTV and dCas9 vectors; digested FTV vector; inoculated LB broth with a colony from the HMF plate for overnight growth and plasmid preparation tomorrow
Wednesday, May 28, 2014
 Prepared plasmid containing the HMF pathway; inoculated LB broth with Lambda Red Recombinase plasmid from cryogenic storage
Thursday, May 29, 2014
 Ashlee and Emily made cryogenic storage of the Lamda Red Recombinase plasmid
Friday, May 30, 2014
Saturday, May 31, 2014
Sunday, June 1, 2014
Monday, June 2, 2014
Tuesday, June 3, 2014
Wednesday, June 4, 2014
Thursday, June 5, 2014
Friday, June 6, 2014
Saturday, June 7, 2014
Sunday, June 8, 2014
Monday, June 9, 2014
Tuesday, June 10, 2014
Wednesday, June 11, 2014
Thursday, June 12, 2014
Friday, June 13, 2014
Saturday, June 14, 2014
Sunday June 15, 2014
Monday, June 16, 2014
Tuesday, June 17, 2014
Wednesday, June 18, 2014
Thursday, June 19, 2014
Friday, June 20, 2014
Saturday, June 21, 2014
Sunday, June 22, 2014
Monday, June 23, 2014

Retrieved from "http://2014.igem.org/Team:Penn_State/Notebook"