OD/F device

Measuring Optical Density (OD) is a central element in microbiological work and synthetic biology in general. Often the qeustion is, how many cells are in a suspension. The OD can give you a hint.

Commercial OD meters cost several hundred dollars ([http://www.laboratory-equipment.com/laboratory-equipment/cell-density-meter.php OD meter]), and can limit the spread of synthetic biology. Especially for BioHack-Spaces, DIY laboratories and schools we wanted to develop an alternative.

With our OD/F device we want to enable many to people for good, precise and cheap science.

Especially for the Interlab Study also fluorescence has been of importance. Here the correlation between OD and fluorescence should be measured. Since the taks of measuring OD and fluorescence are often performed at the same time, we want to present a device that can measure both with easy changes. Finally we can tell you, how much fluorescence there is per amount of cells.

In fact, you can find some DIY posts for turbidity meters such as [http://www.thingiverse.com/thing:74415 Turbidity sensors]. However a proper assessment of their linearity as well as a calculated OD-value is missing.

Also regarding fluorescence, we're not re-inventing the wheel (well, not totally). The 2010 iGEM Cambridge team actually build a very similar device, the E.glometer. However, there's no data available showing an actual comparison of the data from their device, and some proven, commercial system, e.g. to assess linearity of the measurement.

OD device

Hint: Building it

If you want to build the OD device, make sure to use the following secret ingredients:

• Filter:
• LED:

F device

Getting your Device

Table 1: Number of pieces, components and costs for building your own OD/F device

Building your own OD/F device

	First we want to assemble the casing. Once you have all the cut parts, you can start to assemble them. For cutting, we really recommend using a laser cutter.
	Attach the cuvette-holder holders such that the cuvette holder is placed directly under the opening hole.
	Next build the lid of the device. At this stage you can already mount the button. We recommend to glue any parts.
	Your lid finally should look like this.
	Next we want to assemble the cuvette holders. On the side with the square hole attach the light-to-frequency sensor with glue. For the OD case place the orange LED opposite, or for fluorescence, the LED in the hole in the bottom. Make sure to close any remaining open hole!
	Your final assembly should then look like this. Now place the correct filter into the cuvette holder, directly in front of the sensor. Make sure that the filter does not degrade due to the glue!
	As the case can be used for both, fluorescence and OD measurement, we use a combined plug. Just three header rows (7 pins) and connect them as we did.
	Now we're doing the wiring. Connect the Arduino 5V and GND such that you have one 5V and one GND line on your breadboard.
	Then connect the button to 5V on the one side, and to GND via a resistor on the other side. Connect this side also to port __ on your Arduino. This will sense the blank. Next connect the display to the Arduino and our connector. See the Fritzing diagram at the bottom for a detailed information.
	Now put everything into the case and ...
	... also place the cuvette holder into the device. Attach the display to the device lid and close the casing.
	Congratulations! You have finished constructing your own OD/F device!