Team:Freiburg/Content/Results/Light system

From 2014.igem.org

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<h1>The light system</h1>
<h1>The light system</h1>

Revision as of 21:01, 9 October 2014

The AcCELLerator

The light system

One advantage of our system, the spacial resolution by infecting only distinct areas in a mammalian cell culture with our viral vector, was archived by the use of different light systems. We managed to induce the expression of the mCAT-1 receptor in non-murine cell lines by illumination with light of a special wave length. Since, the viral vector only infect cells that express the mCAT-1 receptor on their surfaces, we transferred genes into distinct areas of a otherwise homogenous tissue using this principle.

Function of blue light system

Function of red light system

Light induced receptor/ blue light

Receptor functionality

SEAP as reporter

MuLV SEAP