Team:Washington/Protocols

From 2014.igem.org

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<h3> Ligation </h3>
<h3> Ligation </h3>
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<h2> <i> Escherichia coli Protocols </i> </h2>
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<h2> <i> Escherichia coli Protocols (XL1-Blue and XL10-Gold) </i> </h2>
<h3> Competent Cell Culturing </h3>
<h3> Competent Cell Culturing </h3>
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<h3> DNA-Extraction and mini-preps </h3>
<h3> DNA-Extraction and mini-preps </h3>
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<h2> <i> Saccharomyces cerevisiae </i> </h2>
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<h3> Glycerol Stocks </h3>
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<h2> <i> Saccharomyces cerevisiae (PYE1 Yeast)</i> </h2>
<h3> Transformations </h3>
<h3> Transformations </h3>
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<h3> OVernight Culturing and Passaging </h3>
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<h3> Glycerol Stocks </h3>
<h2> Flow Cytometry and Fluorescence Activated Cell Sorting </h2>
<h2> Flow Cytometry and Fluorescence Activated Cell Sorting </h2>
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<h2> Circluar Dichroism, Thermal Melts and Guandinium Hydrogen Chloride Melts </h2>
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<h3> Dilutions </h3>
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<h3> Final Preparations </h3>
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<h2> Circluar Dichroism, Thermal Melts and Guandinium Hydrogen Chloride Melts </h2>
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Revision as of 18:20, 9 October 2014

UW Homepage Official iGEM website

Contents

Protocols

Media, Plates and Solutions

CCMB

LB-Agar

Luria Broth

Super Optimal Broth (S.O.B.)

Basic Cloning

Polymerase Chain Reactions

Restriction Endonuclease Reaction (Digestion)

Ligation

Escherichia coli Protocols (XL1-Blue and XL10-Gold)

Competent Cell Culturing

Transformations

Plating

Overnights

DNA-Extraction and mini-preps

Glycerol Stocks

Saccharomyces cerevisiae (PYE1 Yeast)

Transformations

OVernight Culturing and Passaging

Glycerol Stocks

Flow Cytometry and Fluorescence Activated Cell Sorting

Dilutions

Final Preparations

Circluar Dichroism, Thermal Melts and Guandinium Hydrogen Chloride Melts

</htlm>

Retrieved from "http://2014.igem.org/Team:Washington/Protocols"