Team:Penn State/Notebook

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   <td><center><b>Friday, May 29, 2014</b></center></td>
   <td><center><b>Friday, May 29, 2014</b></center></td>
   <td>Ashlee and Emily made cryogenic storage of the Lamda Red Recombinase plasmid</td>
   <td>Ashlee and Emily made cryogenic storage of the Lamda Red Recombinase plasmid</td>
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  <td><center><b>Saturday, May 30, 2014</b></center></td>
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  <td><center><b>Sunday, May 31, 2014</b></center></td>
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  <td><center><b>Monday, June 1, 2014</b></center></td>
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Revision as of 17:18, 19 June 2014


WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Human Practices Attributions

Penn State iGEM 2014 Notebook Page

Here you will find weekly summaries of our wet laboratory progress, team updates, and accomplishments outside the laboratory. Below [link this] is our detailed, day-to-day progress laboratory notebook.

Weekly Summaries

will create links to each week, but will just list down the page Week 1, Week 2, etc etc

Laboratory Notebook

Biodetoxification
Codon Optimization
Wednesday, May 21, 2014
 Emily's first experience with cloning! Ashlee led Emily through several practice experiments from designs made earlier in the year: making a gel, loading samples, gel purifying DNA ...................
Thursday, May 22, 2014
 Ashlee and Emily performed a transformation
Friday, May 23, 2014
 We picked several colonies for overnight growth to do more cloning tomorrow
Saturday, May 24, 2014
 Memorial Day Weekend? How about lab cloning weekend! Ashlee conducted plasmid preparation and digestion
Monday, May 25, 2014
 Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the P. putida genome using homologous recombination. Ordered
Tuesday, May 26, 2014
 Inoculated LB broth with ampicillin and dCas9 plasmid from cryogenic storage; inoculated Lb broth with chloramphenicol and FTV vector from Ashlee's past experiment; streaked the HMF vector on a kanamycin plate
Wednesday, May 27, 2014
 Emily and Ashlee made cryogenic storage of the dCas9 plasmid; plasmid prepared the FTV and dCas9 vectors; digested FTV vector; inoculated LB broth with a colony from the HMF plate for overnight growth and plasmid preparation tomorrow
Thursday, May 28, 2014
 Prepared plasmid containing the HMF pathway; inoculated LB broth with Lambda Red Recombinase plasmid from cryogenic storage
Friday, May 29, 2014
 Ashlee and Emily made cryogenic storage of the Lamda Red Recombinase plasmid
Saturday, May 30, 2014
Sunday, May 31, 2014
Monday, June 1, 2014

Retrieved from "http://2014.igem.org/Team:Penn_State/Notebook"