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Team:Pitt/HSP60 Promoter/Intro
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Revision as of 16:44, 9 October 2014
HSP60 Promoter Intro
To reach high levels of protein expression, the Pitt iGEM Team is isolating a high-copy promoter, HSP60, for our BioBrick parts. The HSP60 protein is a ubiquitous chaperone, helping proteins fold correctly, and is necessary for cell survival. Therefore, the HSP60 promoter needs to produce a large amount of the HSP60 protein to prevent other critical proteins from misfolding. Consequently, the HSP60 promoter is an excellent choice for maximum expression BioBrick parts. The Pitt iGEM is using the HSP60 promoter in devices to balance skin oil and to produce melanin.
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