Team:Oxford/biosensor characterisation
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[2] Lopes, N., et al “Detection of dichloromethane with a bioluminescent (lux) bacterial bioreporter” J Ind Microbiol Biotechnol (2012) 39:45–53 | [2] Lopes, N., et al “Detection of dichloromethane with a bioluminescent (lux) bacterial bioreporter” J Ind Microbiol Biotechnol (2012) 39:45–53 | ||
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<h1>Characterising the DcmR - DCM - P_dcmA interaction</h1> | <h1>Characterising the DcmR - DCM - P_dcmA interaction</h1> | ||
To find out whether the gene <font style="font-style: italic;">dcmR</font> acts as a repressor or an activator on the promoter of the <font style="font-style: italic;">dcmA</font> gene, we attempted to build the genetic circuit shown above on the right. Having <font style="font-style: italic;">dcmR</font> under inducible TetR expression should allow us to have very good control of the amount of DcmR present. On top of this, attaching the mCherry fluorescence tag will act as another confirmation to the amount of DcmR present. | To find out whether the gene <font style="font-style: italic;">dcmR</font> acts as a repressor or an activator on the promoter of the <font style="font-style: italic;">dcmA</font> gene, we attempted to build the genetic circuit shown above on the right. Having <font style="font-style: italic;">dcmR</font> under inducible TetR expression should allow us to have very good control of the amount of DcmR present. On top of this, attaching the mCherry fluorescence tag will act as another confirmation to the amount of DcmR present. | ||
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We modelled this first step using both deterministic and stochastic models. | We modelled this first step using both deterministic and stochastic models. | ||
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+ | <div class="white_news_block2"> | ||
+ | <h1>Biochemical equations</h1> | ||
+ | The biochemical equations that describe the behaviour of the top half of the genetic circuit are: | ||
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+ | </div> | ||
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Revision as of 16:25, 9 October 2014
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