"
Team:NCTU Formosa/Notebook/test5
From 2014.igem.org
Alex19950425 (Talk | contribs) |
Alex19950425 (Talk | contribs) |
||
| Line 73: | Line 73: | ||
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
| + | <p> | ||
8/11 | 8/11 | ||
Transformation of NCTU1 (G1) | Transformation of NCTU1 (G1) | ||
Transformation of PBAN (G2) | Transformation of PBAN (G2) | ||
| - | + | </p> | |
| + | <p> | ||
8/12 | 8/12 | ||
Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1) | Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1) | ||
| Line 82: | Line 84: | ||
Mini-prep of [Pcons]Ar | Mini-prep of [Pcons]Ar | ||
Digestion of [Pcons]Ar (ES) (G2) | Digestion of [Pcons]Ar (ES) (G2) | ||
| - | + | </p> | |
| + | <p> | ||
8/13 | 8/13 | ||
Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1) | Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1) | ||
Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1) | Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1) | ||
Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2) | Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2) | ||
| - | + | </p> | |
| + | <p> | ||
8/14 | 8/14 | ||
Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1) | Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1) | ||
| Line 93: | Line 97: | ||
Transformation of the ligation product made yesterday | Transformation of the ligation product made yesterday | ||
Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2) | Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2) | ||
| - | + | </p> | |
| + | <p> | ||
8/15 | 8/15 | ||
Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1) | Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1) | ||
Purification from electrophoresis:[PBAN]No.4&5&6 | Purification from electrophoresis:[PBAN]No.4&5&6 | ||
Transformation of [B0034]Ar (G2) | Transformation of [B0034]Ar (G2) | ||
| - | + | </p> | |
| + | <p> | ||
8/16 | 8/16 | ||
Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1) | Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1) | ||
| Line 106: | Line 112: | ||
Ligation:[Pcons]+[B0034] +PSB1K3 | Ligation:[Pcons]+[B0034] +PSB1K3 | ||
Digestion of [BFP](ES) | Digestion of [BFP](ES) | ||
| - | Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons].8/17[B0034].[BFP] (G2) | + | Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons].8/17[B0034].[BFP] (G2)</p> |
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
Revision as of 15:57, 9 October 2014
- Sunday
- Monday
- Tuesday
- Wednesday
- Thursday
- Friday
- Saturday
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 16
- 17
- 18
- 19
- 20
- 21
- 22
- 23
- 24
- 25
- 26
- 27
- 28
- 29
- 30
- 31
8/11 Transformation of NCTU1 (G1) Transformation of PBAN (G2)
8/12 Cultivation of [PBAN-4]Kr,[PBAN-5]Krand [PBAN-6]Kr E.coli in LB broth (G1) Cultivation of [RBS+BFP+J61048]Cr ,[T7+RBS+BFP+J61048]Ar Kr Mini-prep of [Pcons]Ar Digestion of [Pcons]Ar (ES) (G2)
8/13 Mini-prep of [PBAN-4] Kr,[PBAN-5] Krand [PBAN-6] Kr (G1) Digestion of [PBAN-4]Kr(EP),[PBAN-5]Kr(EP)and [PBAN-6]Kr(EP) (G1) Electrophoresis of [Pcons](mini)&(dig ES),digestion of [Pcons](XP),Ligation:[Pcons]+[RBS+BFP+J61048] (G2)
8/14 Electrophoresis of plasmid and digestion products of [PBAN-4] Kr(EP),[PBAN-5] Kr(EP)and [PBAN-6] Kr(EP) (G1) Purification from electrophoresis:[PBAN]No.4&5&6(G1) Transformation of the ligation product made yesterday Electrophoresis of plasmid and digestion products of [PBAN]No.4&5&6,[Pcons],[Pcons+RBS+BFP+J61048] (G2)
8/15 Digestion of purification products yesterday:[PBAN]No.4&5&6 (EP) (G1) Purification from electrophoresis:[PBAN]No.4&5&6 Transformation of [B0034]Ar (G2)
8/16 Electrophoresis of the purification products from electrophoresis and digestion products:[PBAN]No.4&5&6 (EP) (G1) PCR:[B0034] Electrophoresis:[PSB1K3](mini)&(dig E),[Pcons](dig SP),PCR products of B0034 Mini-prep of [B0034]Ar E.coli Ligation:[Pcons]+[B0034] +PSB1K3 Digestion of [BFP](ES) Electrophoresis of plasmid and digestion products of [PSB1K3],[PSB1C3],5/12[J61048],[Pcons].8/17[B0034].[BFP] (G2)
