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Team:BostonU/Protocols
From 2014.igem.org
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| - | CIP dephosphorylation<br> | + | <a href="https://static.igem.org/mediawiki/2014/4/44/CIP_BU14.xls">CIP dephosphorylation</a><br> |
| - | Colony PCR<br> | + | <a href="https://static.igem.org/mediawiki/2014/0/01/Colony_PCR_BU14.xls">Colony PCR</a><br> |
| - | Epoch spin miniprep<br> | + | <a href="https://static.igem.org/mediawiki/2014/b/b1/Epoch_Miniprep_BU14.xls">Epoch spin miniprep</a><br> |
| - | Epoch spin PCR cleanup<br> | + | <a href="https://static.igem.org/mediawiki/2014/a/a1/Epoch_PCR_cleanup_BU14.xls">Epoch spin PCR cleanup</a><br> |
| - | Ligation<br> | + | <a href="https://static.igem.org/mediawiki/2014/e/e5/Ligation_BU14.xls">Ligation</a><br> |
| - | Media preparation<br> | + | <a href="https://static.igem.org/mediawiki/2014/4/47/Media_Preparation_LB_broth_agar_Gels_BU14.xls">Media preparation</a><br> |
| - | MoClo<br> | + | <a href="https://static.igem.org/mediawiki/2014/d/d8/MoClo_Level_1_and_2_BU14.xls">MoClo Level 1 and 2</a><br> |
| - | NanoDrop quantification<br> | + | <a href="https://static.igem.org/mediawiki/2014/b/ba/NanoDrop_DNA_Quantification_BU14.xls">NanoDrop quantification</a><br> |
| - | Phusion PCR<br> | + | <a href="https://static.igem.org/mediawiki/2014/b/b3/Phusion_PCR_BU14.xls">Phusion PCR</a><br> |
| - | Restriction Digest<br> | + | <a href="https://static.igem.org/mediawiki/2014/1/17/Restriction_Digest_BU14.xls">Restriction Digest</a><br> |
| - | Transformation<br><br> | + | <a href="https://static.igem.org/mediawiki/2014/6/6a/Transformation_BU14.xls">Transformation</a><br><br> |
Flow cytometry workflow | Flow cytometry workflow | ||
</table> | </table> | ||
Revision as of 14:16, 9 October 2014
| The protocols used in our cloning process are listed below. These are standard versions of the protocols mentioned in our notebooks, and we hope that they will be of use to anyone using the Chimera workflow. We have also included a 3-day flow cytometry workflow for setting up samples for characterization. |
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CIP dephosphorylation Colony PCR Epoch spin miniprep Epoch spin PCR cleanup Ligation Media preparation MoClo Level 1 and 2 NanoDrop quantification Phusion PCR Restriction Digest Transformation Flow cytometry workflow |
