Team:Penn State/Notebook

From 2014.igem.org

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   <td><center><b>Monday, May 25, 2014</b></center></td>
   <td><center><b>Monday, May 25, 2014</b></center></td>
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   <td>Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the <i>P. putida</i> genome using homologous recombination.</td>
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   <td>Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the <i>P. putida</i> genome using homologous recombination. Ordered </td>
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  <td><center><b>Tuesday, May 26, 2014</b></center></td>
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  <td>Inoculated LB broth with ampicillin and dCas9 plasmid from cryogenic storage; inoculated Lb broth with chloramphenicol and FTV vector from Ashlee's past experiment; streaked the HMF vector on a kanamycin plate</td>
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  <td><center><b>Wednesday, May 27, 2014</b></center></td>
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  <td>Emily and Ashlee made cryogenic storage of the dCas9 plasmid; plasmid prepared the FTV and dCas9 vectors; digested FTV vector; inoculated LB broth with a colony from the HMF plate for overnight growth and plasmid preparation tomorrow</td>
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Revision as of 04:59, 19 June 2014


WELCOME TO iGEM 2014!

Your team has been approved and you are ready to start the iGEM season!
On this page you can document your project, introduce your team members, document your progress
and share your iGEM experience with the rest of the world!


Click here to edit this page!

Home Team Official Team Profile Project Parts Modeling Notebook Safety Human Practices Attributions

Penn State iGEM 2014 Notebook Page

Biodetoxification
Codon Optimization
Wednesday, May 21, 2014
 Emily's first experience with cloning! Ashlee led Emily through several practice experiments from designs made earlier in the year: making a gel, loading samples, gel purifying DNA ...................
Thursday, May 22, 2014
 Ashlee and Emily performed a transformation
Friday, May 23, 2014
 We picked several colonies for overnight growth to do more cloning tomorrow
Saturday, May 24, 2014
 Memorial Day Weekend? How about lab cloning weekend! Ashlee conducted plasmid preparation and digestion
Monday, May 25, 2014
 Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the P. putida genome using homologous recombination. Ordered
Tuesday, May 26, 2014
 Inoculated LB broth with ampicillin and dCas9 plasmid from cryogenic storage; inoculated Lb broth with chloramphenicol and FTV vector from Ashlee's past experiment; streaked the HMF vector on a kanamycin plate
Wednesday, May 27, 2014
 Emily and Ashlee made cryogenic storage of the dCas9 plasmid; plasmid prepared the FTV and dCas9 vectors; digested FTV vector; inoculated LB broth with a colony from the HMF plate for overnight growth and plasmid preparation tomorrow

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