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Team:Macquarie Australia
From 2014.igem.org
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The 2014 Macquarie University iGEM team is continuing the work of the 2013 team | The 2014 Macquarie University iGEM team is continuing the work of the 2013 team | ||
| - | to synthetically construct the chlorophyll biochemical pathway in <i> Escherichia coli E. coli </i> using | + | to synthetically construct the chlorophyll biochemical pathway in <i> Escherichia coli (E. coli) </i> using |
synthetic Biobricks from <i> Chlamydomonas reinhardtii </i>. The Biobricks from 2013 have | synthetic Biobricks from <i> Chlamydomonas reinhardtii </i>. The Biobricks from 2013 have | ||
been made using Gibson Assembly. Our aim for 2014 is to improve the Biobricks using | been made using Gibson Assembly. Our aim for 2014 is to improve the Biobricks using | ||
Revision as of 03:41, 9 October 2014
- Intro
- Situation
- Idea
- Outcome
Our Project
Chlorophyll is a core component in the process of photosynthesis. As a pigment, it harvests light and plays a primary role in the excitation transfer of energy (Eichwurzel, Stiel et al. 2000), which is vital for plant reproduction and survival (Uliana, Pires et al. 2014). The chlorophyll biochemical pathway is an oxygenic photosynthetic process that oxidizes water to produce hydrogen ions. Thirteen genes govern the five-step pathway and each has a specific role.
The 2014 Macquarie University iGEM team is continuing the work of the 2013 team to synthetically construct the chlorophyll biochemical pathway in Escherichia coli (E. coli) using synthetic Biobricks from Chlamydomonas reinhardtii . The Biobricks from 2013 have been made using Gibson Assembly. Our aim for 2014 is to improve the Biobricks using synthetic techniques, which will be assembled into three functional operons and expressed in E. coli competent cells.
A huge thank you to our sponsors!
