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Team:Evry/Interlab Study/09-18-2014
From 2014.igem.org
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<i>PSB1C3+E0240+J23101</i>: <br> | <i>PSB1C3+E0240+J23101</i>: <br> | ||
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<li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | <li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | ||
<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h43-23h43). | <li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h43-23h43). | ||
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<img src="" alt="PLAN DE PLAQUE" /> | <img src="" alt="PLAN DE PLAQUE" /> | ||
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<i>PSB1C3+E0240+J23102</i>: <br> | <i>PSB1C3+E0240+J23102</i>: <br> | ||
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | ||
| + | <li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30). | ||
| + | <img src="" alt="PLAN DE PLAQUE" /> | ||
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Revision as of 14:54, 8 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101:
PSB1C3+E0240+J23102:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23104:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23105:
glycerol stock preparation from LB culture of the 17th September.
PSB1C3+E0240+J23107:
glycerol stock preparation from LB culture of the 17th September.
PSB1C3+E0240+J23110:
Pre-culture does'nt grow. The TECAN measure is reported.
PSB1C3+E0240+J23115:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23116:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23117:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
Sep 18
