Team:TCU Taiwan/Notebook

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<img src="

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Notebook

<img src="

" width="90%">

<tbody>

<a href="https://2014.igem.org/Team:TCU_Taiwan">Home</a>

<a href="#" style="cursor:default">Team</a>
- <a href="https://2014.igem.org/Team:TCU_Taiwan/Team">Team Members</a>
- <a href="https://igem.org/Team.cgi?id=1473" target="_blank">Official Team Profile</a>
- <a href="https://2014.igem.org/Team:TCU_Taiwan/Contact">Contact</a>

<a href="#" style="cursor:default">Project</a>
- <a href="https://2014.igem.org/Team:TCU_Taiwan/Project">Project</a>
- <a href="https://2014.igem.org/Team:TCU_Taiwan/Parts">Parts</a>
- <a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics">Human Pratics</a>

<a href="https://2014.igem.org/Team:TCU_Taiwan/Modeling">Modeling</a>

<a href="https://2014.igem.org/Team:TCU_Taiwan/Safety">Safety</a>

<a href="https://2014.igem.org/Team:TCU_Taiwan/Notebook" style="color:#DBAC52">Notebook</a>

<a href="https://2014.igem.org/Team:TCU_Taiwan/Attributions">Attributions</a>

<a href="https://2014.igem.org/Main_Page"><img src="" width="40px"></a>

</tbody></table>




Notebook
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.

<img src="

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-    <div id="accordion">
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 1</font><font face="Trebuchet MS" size="6" color="#90B849"> 6/22~6/28</font></h6>
-        <div align="left">
-          <p><h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5><br>
-          <font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell.</font>
-          <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br>
-          Transformation ( Cas9 in pSB1C3 into DH5α).<br>
-          Incubate DH5α with pSB1C3</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α.</font>          </p>
-          <p><h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5><br>
-          <font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt).<br>
-          Practice presentation.<br>
-          Design gRNA for AmpR.<br>
-          Discuss about project. Project Name is E.Maat for now (came up by Sharon).<br>
-          Order restraction enzyme(PstI、EcoRI、XbaI、SpeI). </font></p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 2</font><font face="Trebuchet MS" size="6" color="#90B849"> 6/29~7/5</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
-          <br>
-          <font face="Trebuchet MS" size="4" color="#333">Successfully transform biobrick BBa_I13521、BBa_ K914003、BBa_ K1218011 into DH5α (plasmid: pSB1C3).<br>
-          Extract pSB1C3 after incubation.<br>
-          Use Enzyme digestion(PstI and EcoRI) to confirm. whether pSB1C3 is successfully extracted.<br>
-          Prepared Cm. plate、LB. plate.<br>
-Prepared competent cell.
-</font></p>
-          <p>&nbsp;</p>
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
-          <br>
-          <p><font face="Trebuchet MS" size="4" color="#333">Get foundation from nearby store (yeah!!!!!)<br>
-            Decided to have iGEM contest in local school as human practice</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Others don’t think E.Maat is a good name, under re-consideration. </font></p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 3</font><font face="Trebuchet MS" size="6" color="#90B849"> 7/6~7/12</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Prepare Cm. plate、LB. plate. </font>
-            </p>
-          </p>
-          <p>
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Something wrong with gRNA for AmpR, redesign</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Second project theme came out by Denise and Regina: Trogene horse. Needs later discuss to decided which one as theme( E.Maat or Trogene Horse)</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Try to get foundation from Biotechnology company on e-mail.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Prepare for igem conference in NCTU-Formosa (ppt). </font></p>
-          </p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 4</font><font face="Trebuchet MS" size="6" color="#90B849"> 7/13~7/19</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Incubate E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 ).</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Extract pSB1C3 BBa_I13521、BBa_ K914003、BBa_ K1218011. </font>
-            </p>
-          </p>
-          <p>
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Final Theme decided: Trogene Horse.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Prepare for igem conference in NCTU-Formosa (ppt).</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333"> Designing team logo and project logo</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">House started working on wiki</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Decided to have a synthetic biology contest in National Hualien Girls’ Senior High School. </font></p>
-          </p>
-        </div>
-       <h6 align="left"> <font face="Trebuchet MS" size="6" color="#A66B38">Week 5</font><font face="Trebuchet MS" size="6" color="#90B849"> 7/20~7/26</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Incubate:</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">2. E.coli top10 with pBluescript II SK (-)</font></p>
-          <p>&nbsp;</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Extract :</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">2. pBluescript</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted. </font>
-            </p>
-          </p>
-          <p>
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Prepare for igem conference in NCTU-Formosa (ppt).
-             </font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333"> Designing team logo and project logo.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">House working on wiki construction</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Prepare for the contest in National Hualien Girls’ Senior High School (ppt).</font></p>
-          </p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 6</font><font face="Trebuchet MS" size="6" color="#90B849"> 7/27~8/2</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Incubate:</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">2. E.coli top10 with pBluescript II SK (-).</font></p>
-          <p>&nbsp;</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Extract :</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">2. pBluescript</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted. </font>
-            </p>
-          </p>
-          <p>
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Prepare for igem conference in NCTU-Formosa (ppt).
-             </font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333"> Have a synthetic biology contest in National Hualien Girls’ Senior High School.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Order primer for gRNA
-            team logo and project logo confirmed. </font></p>
-          </p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 7</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/3~8/7</font></h6>
-        <div align="left">
-          <h5>
-          <p><font face="Trebuchet MS" size="4" color="#E72B33">Attending iGEM conference in NCTU-Formosa. </font>
-            </p>
-          </p>
-          <p><img src="https://static.igem.org/mediawiki/2014/4/47/1621918_793376414029197_3582457878420185460_n.jpg" width="80%"></p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 8</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/10~16</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Incubate:</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">2. E.coli top10 with pBluescript II SK (-)</font></p>
-          <p>&nbsp;</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Extract :</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">2. pBluescript</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Purification of RFP sequence from pSB1C3
-            Prepare Cm. plate、LB. plate</font>.</p>
-          <p>
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Team uniform under design.</font></p>
-          <p>
-            <img src="https://static.igem.org/mediawiki/2014/d/d0/TCU_uniform_2014.jpg" width="40%"></p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 9</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/17~8/23</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Incubate:</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">2. E.coli top10 with pBluescript II SK (-)</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Extract :</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">2. pBluescript</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Purification of RFP sequence from pSB1C3. </font>
-            </p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 10</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/24~8/30</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Ligation of RFP and pBluescript SK(-)</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Transformation clone RFP/pBluescript SK(-) to DH5α.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333"> Extract plasmid clone RFP/pBluescript SK(-)</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted. </font>
-            </p>
-          </p>
-          <p>
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Design badge as small gift</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Order Helper phage for pBluescript SK(-).</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Make movie for project introduction of Trogene horse</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Prepare iGEM presentation ppt、poster. </font></p>
-          </p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 11</font><font face="Trebuchet MS" size="6" color="#90B849"> 8/31~9/6</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Incubate:</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">1. E.coli DH5α with BBa_I13521、(in pSB1C3 )</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">2. E.coli top10 with pBluescript II SK (-)</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Extract :</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">1. pSB1C3: BBa_I13521</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">2. pBluescript</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Purification of RFP sequence from pSB1C3.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Ligation Clone RFP/pBluescript SK(-)to DH5α
-            Store BBa_I13521、BBa_ K914003、BBa_ K1218011、clone RFP/pBluescript SK(-). </font>
-            </p>
-          </p>
-          <p>
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Make movie for project introduction of Trogene</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333"> horse
-            Prepare iGEM presentation ppt、poster. </font></p>
-          </p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 12</font><font face="Trebuchet MS" size="6" color="#90B849"> 9/7~9/13</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Ligation Clone RFP/pBluescript SK(-)</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid clone RFP/pBluescript SK(-)</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Modeling for phage injection (Thanks for NCTU-Formosa’s help)</font>.</p>
-          <p>
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
-          <br>
-          <font face="Trebuchet MS" size="4" color="#333">Prepare iGEM presentation ppt、poster. </font>
-          </p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 13</font><font face="Trebuchet MS" size="6" color="#90B849"> 9/14~9/20</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Ligation Clone RFP/pBluescript SK(-)</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Extractplasmid clone RFP/pBluescript SK(-)</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Prepare competent cell(JM101).</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">incubate:</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">1. E.coli DH5α with BBa_I13521(pBluescript)、BBa_K1473001~3003(in pSB1C3 )</font></p>
-          <p>&nbsp;</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Extract :</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">1. pSB1C3: BBa_I13521、BBa_K1473001~3003</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333"> PCR: tracrRNA、Cas 9+crRNA</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Modeling for phage injection. </font>
-            </p>
-          </p>
-          <p>
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Prepare iGEM presentation ppt、poster</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Wiki construction.</font></p>
-          </p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 14</font><font face="Trebuchet MS" size="6" color="#90B849"> 9/21~9/27</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Ligation gRNA/ pSB1C3</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Transformation gRNA/ pSB1C3 to DH5α.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Incubate gRNA/ pSB1C3 in E.coli JM101.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid gRNA/ pSB1C3 in E.coli JM101.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">PCR trRNA、Cas 9+crRNA</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Prepare modeling</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">3 new biobrick finished. </font>
-            </p>
-          </p>
-          <p>
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Have a iGEM contest(HP) in Tzu chi university</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Prepare presentation ppt、poster.</font></p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Wiki construction. </font></p>
-          </p>
-        </div>
-        <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 15</font><font face="Trebuchet MS" size="6" color="#90B849"> 9/28~10/4</font></h6>
-        <div align="left">
-          <h5><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font></h5>
-          <p><br>
-            <font face="Trebuchet MS" size="4" color="#333">Prepare iGEM presentation ppt、poster</font>.</p>
-          <p><font face="Trebuchet MS" size="4" color="#333">Wiki construction. </font></p>
-          </p>
-        </div>
-    </div>
-</div></td>
    </tr>
    <tr>