Team:Aalto-Helsinki/Journal

From 2014.igem.org

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       <li>Personal protective wear: safety goggles, lab coats and gloves
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       <li>Personal protective wear: safety goggles, lab coats and gloves</li>
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       <li>General safety: acting in case of an accident, where to find emergency showers and fire extinguishers, understanding safety labels</li>
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       <li>GMO safety: putting all waste that has been in contact with bacteria (living or dead) to bacterial waste, aseptical working methods</li>
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       In our iGEM work we have only worked with non-toxic E. coli (K12 derivatives) and all the protein coding sequences that we have used in our project are non-hazardous. We did not create new protein coding sequences but instead we used sequences that are already available as Biobricks.
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       In our iGEM work we have only worked with non-toxic <i>E. coli</i> (K12 derivatives) and all the protein coding sequences that we have used in our project are non-hazardous. We did not create new protein coding sequences but instead we used sequences that are already available as Biobricks.
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    <h3>Lab Close-Ups</h3>
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         <p>Because chimera is a term both in gene techonology and mythology, Laura decided (and others immediately agreed) that drawing a chimera for each member would be a cool idea. Everyone could decide three animals and a chimera would be drawn based on those.</p>
         <p>Because chimera is a term both in gene techonology and mythology, Laura decided (and others immediately agreed) that drawing a chimera for each member would be a cool idea. Everyone could decide three animals and a chimera would be drawn based on those.</p>
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         <p>To read more about the chimeras, head to <a href="https://2014.igem.org/Team:Aalto-Helsinki/Outreach#chimeras">"chimeras" section on the Outreach page</a>.</p>
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         <p>//KOLLAASIKUVATÄHÄNTULEE/ tai linkki kimeera-tekstiin</p>
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Revision as of 14:07, 8 October 2014

Journal

We made sure we documented everything important. The summer was full of different experiences and a lot of hard work. You can browse through our work flow here.

There and Back Again
an iGEM team's tale by Aalto-Helsinki

We worked hard but it all paid off. We had fun, we made new friends. We did something new, we learned something new. It was an amazing journey.

We also have more details to share, if you have the interest to read it all (pdf files inc)!

A Scientist discovers that which exists; an Engineer creates that which never was. Theodore von Karmen

Safety

General Safety Matters

All members of our team, who work in the lab, have had a safety training as a part of their studies. The training covered the following topics:

  • Personal protective wear: safety goggles, lab coats and gloves
  • General safety: acting in case of an accident, where to find emergency showers and fire extinguishers, understanding safety labels
  • Chemical safety: working with different chemicals and how to dispose of them
  • GMO safety: putting all waste that has been in contact with bacteria (living or dead) to bacterial waste, aseptical working methods

In our iGEM work we have only worked with non-toxic E. coli (K12 derivatives) and all the protein coding sequences that we have used in our project are non-hazardous. We did not create new protein coding sequences but instead we used sequences that are already available as Biobricks.

Our Laboratory

We got to work in the Bioprocess Technology lab of Aalto University. We had our own table and we got to use all the instruments in the department. We also did some measurements in the labs of VTT Technical Research Centre of Finland.

Here are some pictures of our journey. Enjoy!



Our Office

Vuori HUB <3

Timeline

  • October 2014
  • Jamboree Bootcamp at Mikkeli Summer Cottage

    25.10.2014

    Perfecting everything and admiring our wiki which is totally done at this point. All's well, we can just relax before the Jamboree.

  • September 2014
  • August 2014
  • Testing the DNA Constructs

    28.8.2014

    Testing the first DNA constructs.

  • Bowling

    26.8.2014

    We plan to go to an escape the room thing, but when all the suitable times are booked, Minttu takes us bowling instead.

  • The Night of the Arts in Helsinki and More Pinball

    21.8.2014, 22.8.2014

    We go visit the observatorium during the Night of the Arts event in Helsinki. There's also massive soap bubbles and a man with a whole band setup in a baby carriage.

    The next day we go back to playing pinball and Crash Team Racing because it was so much fun last week.

  • Testing Starts!

    19.8.2014

    We build the first part of the prototype and start testing!

  • Our Synthetized Genes Arrive!

    18.8.2014

    After a long while, our genes finally arrive. Now we can take them and other parts we've assembled and the whole genetically engineered machine together.

  • Meeting at Heureka Science Center!

    18.8.2014

    We meet the event manager and event organizer of Heureka and come up with a synthetic biology weekend workshop we could organize in Spring.

    They were very excited about us and everything we had to show and tell. They said that this is a perfect match from them and all the simulations, games, pictures fit perfectly into the workshop.

    We hope to inspire young people to study synthetic biology in the future and let the older people know it exists. This will also be a perfect opportunity for the next year's iGEM Team to get involved and organize something together.

  • Pinball Night

    14.8.2014

    Lassi takes us to play pinball. It's surprisingly fun. We play a ton of Crash Team Racing too.

  • HYBER Takes Interest In Us!

    8.8.2014

    We meet with The Academy of Finland's Center of Excellence HYBER 2014-2019. They want us to talk in their conference and support us.

    In the evening Oskari takes us to play laser tag! He is way better at it than anyone else.

  • The First Sequencing Results Arrive!

    5.8.2014

    We get our first sequencing results that are in confusing .ab1-files. Then it turns out that Geneious makes analysing them incredibly easy. Most of the sequences look good, some not so much.

  • Friday Sushi Lunch

    1.8.2014

    Instead of staying in our HQ and eating in the cafeteria next to it, Laura takes us to eat fancy sushi. It's delicious and fun.

    SUSHIKUVA?

  • July 2014
  • Building the Synthesizable Sequences

    31.7.2014

    We build the sequences to synthetize from scratch. They include the lambda repressor OR sites that we designed. We find out that the synthetisizing costs the same for 0-1000 nucleotides, so we include a few surrounding BioBricks to save on lab time. (The OR sites are fairly small.)

  • The First Ligations Are Sent to Be Sequenced

    28.7.2014

    We have a number of ligations ready for assembling and it turns out there's a sequencing place like 5 kilometers away.

    We seal 16 samples into tubes and send them off. We are left eagerly waiting to see if the work this far has succeeded.

  • Meeting with Tania

    22.7.2014

    We meet Tania, an ex-iGEMer from the one 2012 Amsterdam team. It's a great meeting and we finally get some validation that we're doing some things right.

    In the evening Martina takes us geocaching! It is amazing what can be hidden in completely plain sight.

  • Demo Day

    15.7.2014

    We present our idea to hundreds of people and hold a booth for 6 hours.

  • A Movie Night with the Team

    14.7.2014

    In the evening Mikko takes us to watch The Skin I Live In, Scott Pilgrim vs. The World and The Grand Budapest Hotel.

  • The Gene Circuit Is Finished

    14.7.2014

    We have the lambda repressor figured out and integrated into our system. We start planning for the parts to be synthesized.

  • Summer of Startups Tallinn Trip!

    10-11.7.2014

    Summer of Startups takes us to visit the Startup people of Tallinn. We decide this is a practice trip for Boston, so we planned to have as much fun as possible. It was a great success and an unforgettable experience.

  • Extensive Research on the Lambda Repressor

    8.7.2014

    We find out that the lambda repressor system would be perfect for our three-way switch idea. We figure out how it works and how to include it in our gene circuit.

  • The "Fun Master" System Begins

    4.7.2014

    We decided that we should do something fun every week as a group that has nothing to do with iGEM. Every team member has their own week to plan an activity and pull the team to the mandatory weekly having-fun moment.

    So, Otto surprised us with a fruit juicer and a ton of fresh fruit. We spent the early morning happily drinking smoothies while working intensely on our project.

  • The First Team Member Chimera

    2.7.2014

    Because chimera is a term both in gene techonology and mythology, Laura decided (and others immediately agreed) that drawing a chimera for each member would be a cool idea. Everyone could decide three animals and a chimera would be drawn based on those.

    //KOLLAASIKUVATÄHÄNTULEE/ tai linkki kimeera-tekstiin

  • Brian Is Born

    1.7.2014, 2.7.2014

    We get the idea of explaining synthetic biology as turning bacteria into superheroes from a meeting with ???. We start working on a new pitch based on that.

    Pietu finishes the pitch the next day and presents it at Summer of Startups. It seems to go over very well. People finally understand what we are doing.

  • June 2014
  • BioBrick Seeker on the iGEM Help and Community Pages

    25.6.2014

    Our BioBrick Seeker has been mentioned on the official help page for a while. We only noticed it now.

    We also wrote a small advertisement text for the Community page and got the Seekers featured there.

  • The First BioBrick Ligation Combo Works

    24.6.2014

    We even checked it with a gel.

    KUVA??

  • Writing Sponsorship Applications

    10.6.2014

    We're trying to advertise ourselves and get some kind of financial help and support for this project. It feels really difficult and time consuming. We also took some new pictures, a new group photo, for example.

  • Trouble in the Lab

    8.6.2014

    The backbones don't seem to be working right and the negative control keeps growing. Not good.

  • BioBrick Seeker spreading

    2.6.2014

    We've received tons of mentions on Twitter. People really seem to like the tool.

  • May 2014
  • Precipitation of the Cell Membranes

    30.5.2014

    This process would be repeated about 50 times during the summer, but this was the first. Video link: http://www.youtube.com/watch?v=TI_EuzNIaPg.

  • The First Version of BioBrick Seeker

    30.5.2014

    It started from the mild inconvenience of searching for BioBricks in the lab. Now we could search through this year's distribution effortlessly. Because it was so useful for us, we decided to share it to others, too.

  • The First BioBrick Transformation in Finland

    26.5.2014

    Cooool. Here it is!

  • Website Is Done

    23.5.2014

    This time it does actually look cool! The actual website might still be available on http://aaltohelsinki.com/index_en.html: check it out!

  • Lab Kicks Off!

    20.5.2014

    ...by making plates. Not that mind-blowing, but very important.

  • A Tour in the Lab

    19.5.2014

    We get one desk from the laboratory. and there's still someone else's stuff on it, but it's ours and we are going to do amazing things there.

  • The iGEM 2014 Distribution Kit Arrives

    9.5.2014

    The competition starts to feel real.

  • April 2014
  • Getting Ready for the Summer of Startups

    22.4.2014, 25.4.2014, 28.4.2014

    We meet a few times to go over what we will say to the Summer of Startups panel, so that they will accept us to the program. This culminates on the 28th, when we have the interview. Our team leader tries to attend through Skype, but the panel doesn't like it. We manage to pull through anyway, and the panel is very excited about us at the end.

    They accepted us in the program.

  • Expert Meeting at Aalto University

    10.4.2014

    We presented ourselves and our initial ideas to a panel of experts, mostly researchers from VTT and the Viikki biocampus. The occasion went very well and there was a lot of interesting discussion. We noticed that we really can do cool things as a group.

  • Meeting with Markus at Helsinki Think Company

    10.4.2014

    We presented our initial ideas to our advisor, Markus, in preparation for the expert meet. We discussed what direction to go and what kind of idea to pick.

  • March 2014
  • Making an Introduction Video

    30.3.2014

    We made an introduction video of us so we could apply to Summer of Startups. It turned out pretty good! Video link: http://www.youtube.com/watch?v=LJX21WthZQg.

  • Hackathon

    23.3.2014

    The first version of our website was built. We wanted it to look cool. It didn't.

  • Throwing Ideas

    18.3.2014, 25.3.2014

    Some brainstorming. Wild ideas. Rating the ideas. Then an unbelievably cool post-it gradient of all the ideas.

  • The First Meeting

    14.3.2014

    The first team meeting was held on a Friday afternoon. Some of us met each other for the first time ever, some had met before already. We talked about the project and got to know each other.

  • Picking the Team

    5.3.2014 - 12.3.2014

    Oskari is in the first job interview in his life, as the interviewer. He holds 19 more interviews during the week. Nine people are chosen to be in the final team.

  • The Beginning
  • Meetings with Aalto University and University of Helsinki

    31.1.2014, 5.2.2014

    It seems like the universities are into the idea.

  • The Idea of a Finnish iGEM Team Forms

    28.8.2013

    First e-mails about the idea are sent.