Week 1 6/22~6/28
experimental part:
Prepare for the competent cell.
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α).
Incubate DH5α with pSB1C3.
Extract plasmid with pSB1C3 from DH5α.
other part:
Prepare Human practice (ppt).
Practice presentation.
Design gRNA for AmpR.
Discuss about project. Project Name is E.Maat for now (came up by Sharon).
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI).
Week 2 6/29~7/5
experimental part:
Successfully transform biobrick BBa_I13521、BBa_ K914003、BBa_ K1218011 into DH5α (plasmid: pSB1C3).
Extract pSB1C3 after incubation.
Use Enzyme digestion(PstI and EcoRI) to confirm. whether pSB1C3 is successfully extracted.
Prepared Cm. plate、LB. plate.
Prepared competent cell.
other part:
Get foundation from nearby store (yeah!!!!!)
Decided to have iGEM contest in local school as human practice.
Others don’t think E.Maat is a good name, under re-consideration.
Week 3 7/6~7/12
experimental part:
Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.
Prepare Cm. plate、LB. plate.
other part:
Something wrong with gRNA for AmpR, redesign.
Second project theme came out by Denise and Regina: Trogene horse. Needs later discuss to decided which one as theme( E.Maat or Trogene Horse).
Try to get foundation from Biotechnology company on e-mail.
Prepare for igem conference in NCTU-Formosa (ppt).
Week 4 7/13~7/19
experimental part:
Incubate E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 ).
Extract pSB1C3 BBa_I13521、BBa_ K914003、BBa_ K1218011.
other part:
Final Theme decided: Trogene Horse.
Prepare for igem conference in NCTU-Formosa (ppt).
Designing team logo and project logo.
House started working on wiki.
Decided to have a synthetic biology contest in National Hualien Girls’ Senior High School.
Week 5 7/20~7/26
experimental part:
Incubate:
1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )
2. E.coli top10 with pBluescript II SK (-)
Extract :
1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011
2. pBluescript
Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
other part:
Prepare for igem conference in NCTU-Formosa (ppt).
Designing team logo and project logo.
House working on wiki construction.
Prepare for the contest in National Hualien Girls’ Senior High School (ppt).
Week 6 7/27~8/2
experimental part:
Incubate:
1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 ).
2. E.coli top10 with pBluescript II SK (-).
Extract :
1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011
2. pBluescript
Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
other part:
Prepare for igem conference in NCTU-Formosa (ppt).
Have a synthetic biology contest in National Hualien Girls’ Senior High School.
Order primer for gRNA
team logo and project logo confirmed.
Week 7 8/3~8/7
Attending iGEM conference in NCTU-Formosa.
Week 8 8/10~16
experimental part:
Incubate:
1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )
2. E.coli top10 with pBluescript II SK (-)
Extract :
1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011
2. pBluescript
Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
Purification of RFP sequence from pSB1C3
Prepare Cm. plate、LB. plate.
other part:
Team uniform under design.
Week 9 8/17~8/23
experimental part:
Incubate:
1. E.coli DH5α with BBa_I13521、BBa_ K914003、BBa_ K1218011(in pSB1C3 )
2. E.coli top10 with pBluescript II SK (-)
Extract :
1. pSB1C3: BBa_I13521、BBa_ K914003、BBa_ K1218011
2. pBluescript
Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
Purification of RFP sequence from pSB1C3.
Week 10 8/24~8/30
experimental part:
Ligation of RFP and pBluescript SK(-).
Transformation clone RFP/pBluescript SK(-) to DH5α.
Extract plasmid clone RFP/pBluescript SK(-).
Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
other part:
Design badge as small gift.
Order Helper phage for pBluescript SK(-).
Make movie for project introduction of Trogene horse.
Prepare iGEM presentation ppt、poster.
Week 11 8/31~9/6
experimental part:
Incubate:
1. E.coli DH5α with BBa_I13521、(in pSB1C3 )
2. E.coli top10 with pBluescript II SK (-)
Extract :
1. pSB1C3: BBa_I13521
2. pBluescript
Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
Purification of RFP sequence from pSB1C3.
Ligation Clone RFP/pBluescript SK(-)to DH5α
Store BBa_I13521、BBa_ K914003、BBa_ K1218011、clone RFP/pBluescript SK(-).
other part:
Make movie for project introduction of Trogene.
horse
Prepare iGEM presentation ppt、poster.
Week 12 9/7~9/13
experimental part:
Ligation Clone RFP/pBluescript SK(-).
Extract plasmid clone RFP/pBluescript SK(-).
Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
Modeling for phage injection (Thanks for NCTU-Formosa’s help).
other part:
Prepare iGEM presentation ppt、poster.
Week 13 9/14~9/20
experimental part:
Ligation Clone RFP/pBluescript SK(-).
Extractplasmid clone RFP/pBluescript SK(-).
Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
Prepare competent cell(JM101).
incubate:
1. E.coli DH5α with BBa_I13521(pBluescript)、BBa_K1473001~3003(in pSB1C3 )
Extract :
1. pSB1C3: BBa_I13521、BBa_K1473001~3003
Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
PCR: tracrRNA、Cas 9+crRNA.
Modeling for phage injection.
other part:
Prepare iGEM presentation ppt、poster.
Wiki construction.
Week 14 9/21~9/27
experimental part:
Ligation gRNA/ pSB1C3.
Transformation gRNA/ pSB1C3 to DH5α.
Incubate gRNA/ pSB1C3 in E.coli JM101.
Extract plasmid gRNA/ pSB1C3 in E.coli JM101.
Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
PCR trRNA、Cas 9+crRNA.
Prepare modeling.
3 new biobrick finished.
other part:
Have a iGEM contest(HP) in Tzu chi university.
Prepare presentation ppt、poster.
Wiki construction.
Week 15 9/28~10/4
other part:
Prepare iGEM presentation ppt、poster.
Wiki construction.
"
