08/08/2014

Heeeyy !! Today remade the plasmid extraction with kit JM110, and DH5α with Biobrick for bioremediation and essential Biobrick.

Eletrophoretical profile of samples

1. Biobrick essencial extraído de DH5α (1)

2. Biobrick essencial extraído de DH5α (2)

3. Biobrick para biorremediação extraído de JM110 (1)

4. Biobrick para biorremediação extraído de JM110 (2)

Após a extração, digerimos as amostras com EcoRI e PstI afim de analisar o tamanho do fragmento do Biobrick essencial e Biobrick para biorremediação. Utilizamos o seguinte sistema para digestão enzimática:

The digestion lasted 2 hours at 37°C. We applied the gel with 0.8% of agarose the total volume of each sample.

Eletrophoretical profile of the digested samples:

1. Bioremediation Biobrick not digested.

2. Bioremediation Biobrick digested with EcoRI and PstI

3. Essential Biobrick not digested.

4. Essential Biobrick digested with EcoRI and PstI.

5-6-7-8. Plasmids did not show a good extraction

Hurray!!! The digestion has released fragments that proved the viability our beloved biobricks. Bioremediation Biobrick has 1.823 base pairs and Essential Biobrickhas 1.234 base pairs,that’s why BB_Biorremediation sits above BB_Essential in the gel. =)

We have also analyzed samples 5-6-7-8 that didn’t show digestion or it showed partial digestion (Either no presented digestion or not presented partial digestion).