Team:Aachen/Notebook/Wetlab2

From 2014.igem.org

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= August =
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== 1st ==
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* made electrocompetent ''E.coli'' rosetta cells.
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* prepared cultures of K1319042 and K131026
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== 2nd ==
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* tested the OD measurement device and compared it to the spectrophotometer and the plate reader.
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* tested K131026 and K1319042 for fluorescence in the plate reader
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* did a heat shock transformation of I746909 into NEB TOP 10 cells
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* did an electroshock transformation of pET17-Gal3 into ''E.coli'' rosetta
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 +
[https://2014.igem.org/Team:Aachen/Wetlab/August Read more about August here...]
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= September =
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== 1st ==
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* 5 ml cultures of K1319003 and K1319004
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* plasmid prep
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{| class="wikitable"
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|-
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! Plasmid !! DNA [ng/µl]
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|-
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| J23101.K516032 pSB1K3|| 23.5
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|-
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| J23115.K516032 pSB1K3|| 20.5
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|-
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| J04450 pSB1A3|| 57.5
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|-
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| J04450 pSB1K1|| 63.5
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|}
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* over night cultures of K131026 in DH5α and NEB
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 +
[https://2014.igem.org/Team:Aachen/Wetlab/September Read more about September here...]
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{{Team:Aachen/BlockSeparator}}
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= October =
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== 1st ==
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* Prepartations for sensor-chip production the following day (2014-10-02) was done accoringly to the [https://2014.igem.org/Team:Aachen/Notebook/Protocols#Agar_Chips| sensor chip manufacturing] protocol:
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** At 18:30 we prepared over-night cultures from K1319042, B0015 and K131026 by inoculating 250 ml Erlenmeyer flasks each containing 50 ml LB medium . The flasks were incubated for ~12 hours at 37 °C on a shaker.
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[https://2014.igem.org/Team:Aachen/Wetlab/October Read more about October here...]
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{{Team:Aachen/Footer}}
{{Team:Aachen/Footer}}

Revision as of 17:41, 7 October 2014

April

1st

Organization

where to get:

  • key cards and keys for the lab
  • cooled centifuge
  • space in -80 °C
  • bottles for 70 % ethanol
  • ... (problems of a first year team ^^)

Read more about April here...

May

1st

  • gel with M - full -full - REACh1 SOE3.2 - REACH2 SOE3.2 - M
    → 120 V, 30 min
    → cut out the bands

Read more about May here...

June

3rd

  • transformation of 34 BioBricks

4th

  • preperation of consumables:
    • fresh 50 % glycerol
    • new LB plates with cam and with kanamycin (kan)
    • 60 glas tubes
    • 2 L LB-
    • 100 mL steril glas beads for plating
  • master plates (6 clones per BioBrick) were made

Read more about June here...

July

1st

  • transformation efficiency kit is broken

→ run an agarose gel with the kit plasmids to check for nuclease activity/ bands

  • inventory of the -80°C freezer was done

2nd

  • overnight cultures (LB) of K731520 and K1319042 for chips were inoculated

Read more about July here...

August

1st

  • made electrocompetent E.coli rosetta cells.
  • prepared cultures of K1319042 and K131026

2nd

  • tested the OD measurement device and compared it to the spectrophotometer and the plate reader.
  • tested K131026 and K1319042 for fluorescence in the plate reader
  • did a heat shock transformation of I746909 into NEB TOP 10 cells
  • did an electroshock transformation of pET17-Gal3 into E.coli rosetta

Read more about August here...

September

1st

  • 5 ml cultures of K1319003 and K1319004
  • plasmid prep
Plasmid DNA [ng/µl]
J23101.K516032 pSB1K3 23.5
J23115.K516032 pSB1K3 20.5
J04450 pSB1A3 57.5
J04450 pSB1K1 63.5
  • over night cultures of K131026 in DH5α and NEB

Read more about September here...

October

1st

  • Prepartations for sensor-chip production the following day (2014-10-02) was done accoringly to the sensor chip manufacturing protocol:
    • At 18:30 we prepared over-night cultures from K1319042, B0015 and K131026 by inoculating 250 ml Erlenmeyer flasks each containing 50 ml LB medium . The flasks were incubated for ~12 hours at 37 °C on a shaker.

Read more about October here...

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