Team:TCU Taiwan/Notebook
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<h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 1</font><font face="Trebuchet MS" size="6" color="#90B849"> 6/22~6/28</font></h6> | <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 1</font><font face="Trebuchet MS" size="6" color="#90B849"> 6/22~6/28</font></h6> | ||
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+ | <p><font face="Trebuchet MS" size="4" color="#0879A6">experimental part:</font><br><font face="Trebuchet MS" size="4" color="#333">Prepare for the competent cell</font> | ||
+ | <p><font face="Trebuchet MS" size="4" color="#333">BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:<br> | ||
+ | Transformation ( Cas9 in pSB1C3 into DH5α)<br>Incubate DH5α with pSB1C3</font></p> | ||
+ | <p><font face="Trebuchet MS" size="4" color="#333">Extract plasmid with pSB1C3 from DH5α</font> </p> | ||
+ | <p><font face="Trebuchet MS" size="4" color="#0879A6">other part:</font><br><font face="Trebuchet MS" size="4" color="#333">Prepare Human practice (ppt)<br>Practice presentation<br>Design gRNA for AmpR<br> | ||
+ | Discuss about project. Project Name is E.Maat for now (came up by Sharon)<br> | ||
+ | Order restraction enzyme(PstI、EcoRI、XbaI、SpeI) </font></p> | ||
+ | </div> | ||
<h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 2</font><font face="Trebuchet MS" size="6" color="#90B849"> 6/29~7/5</font></h6> | <h6 align="left"><font face="Trebuchet MS" size="6" color="#A66B38">Week 2</font><font face="Trebuchet MS" size="6" color="#90B849"> 6/29~7/5</font></h6> |
Revision as of 13:54, 7 October 2014