Team:ZJU-China/Project

Project Description

The assembly of genetic circuits is a huge obstacle between designs and achievements in synthetic biology. Many well-known methods, from traditional restriction digestion & ligation, 3A assembly to Gibson assembly, aim to overcome the difficulties but unfortunately get respective defects. This year, ZJU-CHINA seeks to build a gene-insertion system in bacterial chromosome, "GeneSocket". Clearly different from the in vitro constructing methods mentioned before, GeneSocket, which can be easily combined with existing in vitro methods, makes gene expression more accurate, stable and controllable by assembling genetic elements in chromosome directly.

Two core methods, lambda red recombination and recombinase-based bistable switch are applied to build our Gene Socket. Lambda red recombination is a widely used, efficient recombination system in prokaryotes. Recombinase-based bistable switch is relatively more stable and easier than transcription factor regulated bistable switch modules. Both the two are the best choices for achieving the characteristics of Gene Socket.

We hope that by using GeneSocket, synthetic biologists can turn their theoretical design into reality faster and better. We want to lead to the revolution in techniques of synthetic biology!

Gene Socket

The assembly of genetic circuits is a huge obstacle between designs and achievements in synthetic biology. Many well-known methods, from traditional restriction digestion & ligation, 3A assembly to Gibson assembly, aim to overcome the difficulties but unfortunately get respective defects. This year, ZJU-CHINA seeks to build a gene-insertion system in bacterial chromosome, "GeneSocket". Clearly different from the in vitro constructing methods mentioned before, GeneSocket, which can be easily combined with existing in vitro methods, makes gene expression more accurate, stable and controllable by assembling genetic elements in chromosome directly.

Two core methods, lambda red recombination and recombinase-based bistable switch, are applied. Both are the best choices for achieving the characteristics of GeneSocket.

We hope that by using GeneSocket, synthetic biologists can turn their theoretical design into reality faster and better. We want to lead to the revolution in techniques of synthetic biology!

Content

You can use these subtopics to further explain your project

1. Overall project summary
2. Project Details
3. Materials and Methods
4. The Experiments
5. Results
6. Data analysis
7. Conclusions

It's important for teams to describe all the creativity that goes into an iGEM project, along with all the great ideas your team will come up with over the course of your work.

It's also important to clearly describe your achievements so that judges will know what you tried to do and where you succeeded. Please write your project page such that what you achieved is easy to distinguish from what you attempted.

References

iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.