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Team:TU Delft-Leiden/WetLab/landmine/characterisation
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As already <a href="/Team:TU_Delft-Leiden/WetLab/landmine">mentioned</a>, the promoters found to be activated in presence of several chemical compounds that can leak from land mines (ybiJ and yqjF) were coupled to the expression of the fluorescent protein mKate2. | As already <a href="/Team:TU_Delft-Leiden/WetLab/landmine">mentioned</a>, the promoters found to be activated in presence of several chemical compounds that can leak from land mines (ybiJ and yqjF) were coupled to the expression of the fluorescent protein mKate2. | ||
| - | < | + | <h2> Assays </h2> |
<p> | <p> | ||
The different assays used to test our Land Mine BioBricks are: | The different assays used to test our Land Mine BioBricks are: | ||
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<h3> Plate Reader </h3> | <h3> Plate Reader </h3> | ||
<p> | <p> | ||
| - | A plate reader is a machine designed to handle samples on 6-1536 well format microtiter plates for the measuring of physical properties such as absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarisation. Concerning this module, the plate reader device was used for the measurement of fluorescence intensity generated by cells carrying the BioBricks designed to detect land mines. | + | A plate reader is a machine designed to handle samples on 6-1536 well format microtiter plates for the measuring of physical properties such as absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarisation. Concerning this module, the plate reader device was used for the measurement of fluorescence intensity generated by cells carrying the BioBricks designed to detect land mines. The protocol followed for this module can be found at |
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| + | <h2> LINK TO PROTOCOLS </h2> | ||
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| + | <h3> Results - Plate Reader </h3> | ||
<a name="LDmicroscopy"></a> | <a name="LDmicroscopy"></a> | ||
Revision as of 15:08, 5 October 2014
Landmine Module - Characterisation
As already mentioned, the promoters found to be activated in presence of several chemical compounds that can leak from land mines (ybiJ and yqjF) were coupled to the expression of the fluorescent protein mKate2.
Assays
The different assays used to test our Land Mine BioBricks are:
Plate Reader
Microscopy
FACS
Plate Reader
A plate reader is a machine designed to handle samples on 6-1536 well format microtiter plates for the measuring of physical properties such as absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarisation. Concerning this module, the plate reader device was used for the measurement of fluorescence intensity generated by cells carrying the BioBricks designed to detect land mines. The protocol followed for this module can be found at
LINK TO PROTOCOLS
Results - Plate Reader
Microscopy
FACS
Fluorescence-activated cell sorting (FACS) is a specialised type of flow cytometry
