"
Team:EPF Lausanne/Parts
From 2014.igem.org
(Difference between revisions)
| Line 267: | Line 267: | ||
<td class="biobrick_name">BBa_K1486028</td> | <td class="biobrick_name">BBa_K1486028</td> | ||
<td>Nterm Yeast sfGFP</td> | <td>Nterm Yeast sfGFP</td> | ||
| - | <td> | + | <td>N terminal moiety of split yeast sfGFP</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486029</td> | <td class="biobrick_name">BBa_K1486029</td> | ||
| - | <td>Nterm sfGFP | + | <td>Nterm sfGFP + kan</td> |
| - | <td>Nterm | + | <td>Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486030</td> | <td class="biobrick_name">BBa_K1486030</td> | ||
| - | <td>Nterm rLuc | + | <td>Nterm rLuc + kan</td> |
| - | <td>Nterm | + | <td>Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
| Line 290: | Line 290: | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486032</td> | <td class="biobrick_name">BBa_K1486032</td> | ||
| - | <td></td> | + | <td>Yeast sfGFP + Ura</td> |
| - | <td></td> | + | <td>Yeast sfGFP attached to the Uracil CDS</td> |
| - | <td></td> | + | <td>Control the expression of hog1</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486033</td> | <td class="biobrick_name">BBa_K1486033</td> | ||
| - | <td></td> | + | <td>rLuc + Ura</td> |
| - | <td></td> | + | <td>Renilla luciferase attached to the Uracil CDS</td> |
| - | <td></td> | + | <td>Control the expression of hog1</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486034</td> | <td class="biobrick_name">BBa_K1486034</td> | ||
| - | <td></td> | + | <td>Cterm yeast sfGFP</td> |
| - | <td></td> | + | <td>C terminal moiety of split the yeast sfGFP</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486035</td> | <td class="biobrick_name">BBa_K1486035</td> | ||
| - | <td></td> | + | <td>Cterm yeast sfGFP + Ura</td> |
| - | <td></td> | + | <td>Cterm moiety of split yeast sfGFP attached to the Uracil CDS</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486036</td> | <td class="biobrick_name">BBa_K1486036</td> | ||
| - | <td></td> | + | <td>Cterm rLuc + Ura</td> |
| - | <td></td> | + | <td>Cterm moiety of split renilla luciferase attached to the Uracil CDS</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486037</td> | <td class="biobrick_name">BBa_K1486037</td> | ||
| - | <td></td> | + | <td>linker</td> |
| - | <td></td> | + | <td>Attaches two proteins together</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486038</td> | <td class="biobrick_name">BBa_K1486038</td> | ||
| - | <td></td> | + | <td>Nterm sfGFP</td> |
| - | <td></td> | + | <td>N terminus moiety of split superfolder GFP</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486039</td> | <td class="biobrick_name">BBa_K1486039</td> | ||
| - | <td></td> | + | <td>Cterm sfGFP</td> |
| - | <td></td> | + | <td>C terminus moiety of split superfolder GFP</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486040</td> | <td class="biobrick_name">BBa_K1486040</td> | ||
| - | <td></td> | + | <td>Nterm sfGFP + CpxR</td> |
| - | <td></td> | + | <td>N terminus moiety of split sfGFP attached to CpxR</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486041</td> | <td class="biobrick_name">BBa_K1486041</td> | ||
| - | <td></td> | + | <td>Cterm sfGFP + CpxR</td> |
| - | <td></td> | + | <td>C terminus moiety of split sfGFP attached to CpxR</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486042</td> | <td class="biobrick_name">BBa_K1486042</td> | ||
| - | <td></td> | + | <td>LZip</td> |
| - | <td></td> | + | <td>Monomer of leucine zipper TF</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td class="biobrick_name">BBa_K1486043</td> | <td class="biobrick_name">BBa_K1486043</td> | ||
| - | <td></td> | + | <td>LZip + split rLuc</td> |
| - | <td></td> | + | <td>Two Leucine Zipper monomers, each attached to a different split rLuc moiety</td> |
| - | <td></td> | + | <td>Control for split rLuc assays</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 09:32, 5 October 2014
PARTS
Parts submitted by the 2014 EPFL iGEM team
Our team submitted a total of 43 Biobricks.
| Biobrick | What it is | Function | Why do we use it? |
|---|---|---|---|
| BBa_K1486000 | CpxR coding sequence | Transcription factor | To make most of our biobricks! |
| BBa_K1486001 | CpxR under arabinose promoter | Treanscription factor | |
| BBa_K1486002 | PAra + sfGFP CpxR | Expresses fused protein | Test CpxR expression & Ara promoter |
| BBa_K1486003 | Flexible linker | Attaches two proteins together | |
| BBa_K1486004 | Flexible linker | Attaches two proteins together | |
| BBa_K1486005 | PAra + CpxR sfGFP | Expresses fused protein | Test CpxR expression & Ara promoter |
| BBa_K1486006 | IFP[1] | N terminus of split IFP | |
| BBa_K1486007 | IFP[2] | C terminus of split IFP | |
| BBa_K1486008 | CxpR & Split IFP1.4 [Cterm + Cterm] | Two CpxR CDS, each C terminus attached to a moiety of IFP | Characterize CpxR dimerization |
| BBa_K1486009 | CxpR & Split IFP1.4 [Nterm + Nterm] | Two CpxR CDS, each N terminus attached to a moiety of IFP | Characterize CpxR dimerization |
| BBa_K1486010 | CxpR & Split IFP1.4 [Nterm + Cterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization |
| BBa_K1486011 | CxpR & Split IFP1.4 [Cterm + Nterm] | Two CpxR CDS, each attached to a moiety of IFP | Characterize CpxR dimerization |
| BBa_K1486012 | CpxR + Nterm IFP | CpxR with the Nterm moiety of IFP attached at its C terminus | Intermediate & control |
| BBa_K1486013 | CpxR + Cterm IFP | CpxR with the Cterm moiety of IFP attached at its C terminus | Intermediate & control |
| BBa_K1486014 | Nterm IFP + CpxR | CpxR with the Nterm moiety of IFP attached at its N terminus | Intermediate & control |
| BBa_K1486015 | Cterm IFP + CpxR | CpxR with the Cterm moiety of IFP attached at its N terminus | Intermediate & control |
| BBa_K1486016 | Nterm fLuc | N terminus moiety of the firefly luciferase | |
| BBa_K1486017 | Cterm fLuc | C terminus moiety of the firefly luciferase | |
| BBa_K1486018 | PAra + Nterm fLuc + Cterm fLuc | Split firefly luciferase under arabinose promoter | Control |
| BBa_K1486019 | Nterm rLuc | C terminus moiety of the renilla luciferase | |
| BBa_K1486020 | Cterm rLuc | N terminus moiety of the renilla luciferase | |
| BBa_K1486021 | PAra + Nterm rLuc + Cterm rLuc | Split renilla luciferase under arabinose promoter | Control |
| BBa_K1486022 | rLuc | Full renilla luciferase | Control |
| BBa_K1486023 | Yeast sfGFP | Superfolder GFP for yeast cells | Reporter |
| BBa_K1486024 | Kan | Yeast kanamycin resistance gene | Selection marker |
| BBa_K1486025 | ADH1 terminator | Terminator | |
| BBa_K1486026 | Yeast sfGFP + Kan | Yeast sfGFP attached to the yeast kanamycin resistance gene | Control the expression of pbs2 |
| BBa_K1486027 | rLuc + Kan | Renilla luciferase attached to the yeast kanamycin resistance gene | |
| BBa_K1486028 | Nterm Yeast sfGFP | N terminal moiety of split yeast sfGFP | |
| BBa_K1486029 | Nterm sfGFP + kan | Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene | |
| BBa_K1486030 | Nterm rLuc + kan | Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene | |
| BBa_K1486031 | Ura | CDS for Uracil (yeast selective purposes) | Confer resistance to Uracil-deprived medium |
| BBa_K1486032 | Yeast sfGFP + Ura | Yeast sfGFP attached to the Uracil CDS | Control the expression of hog1 |
| BBa_K1486033 | rLuc + Ura | Renilla luciferase attached to the Uracil CDS | Control the expression of hog1 |
| BBa_K1486034 | Cterm yeast sfGFP | C terminal moiety of split the yeast sfGFP | |
| BBa_K1486035 | Cterm yeast sfGFP + Ura | Cterm moiety of split yeast sfGFP attached to the Uracil CDS | |
| BBa_K1486036 | Cterm rLuc + Ura | Cterm moiety of split renilla luciferase attached to the Uracil CDS | |
| BBa_K1486037 | linker | Attaches two proteins together | |
| BBa_K1486038 | Nterm sfGFP | N terminus moiety of split superfolder GFP | |
| BBa_K1486039 | Cterm sfGFP | C terminus moiety of split superfolder GFP | |
| BBa_K1486040 | Nterm sfGFP + CpxR | N terminus moiety of split sfGFP attached to CpxR | |
| BBa_K1486041 | Cterm sfGFP + CpxR | C terminus moiety of split sfGFP attached to CpxR | |
| BBa_K1486042 | LZip | Monomer of leucine zipper TF | |
| BBa_K1486043 | LZip + split rLuc | Two Leucine Zipper monomers, each attached to a different split rLuc moiety | Control for split rLuc assays |
Sponsors
Copyright © iGEM EPFL 2014. All Rights Reserved
