Team:Vanderbilt/Notebook

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(Spring 2014)
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<div style="color:black;font-size:11pt">'''March 31th'''</div>
<div style="color:black;font-size:11pt">'''March 31th'''</div>
* Ran a 0.6 % argarose gel on the DNA extracted from ZIN and CAR, as well as the column flow-through from the kit.  
* Ran a 0.6 % argarose gel on the DNA extracted from ZIN and CAR, as well as the column flow-through from the kit.  
-
* ZIN1, ZIN2, CAR1, and CAR2 all show a distinct DNA band above the highest rung on the DNA ladder (>10kb), showing the presence of DNA. No bands were seen on the kit flow-through. Indicates successful genomic extraction.  
+
* ZIN1, ZIN2, CAR1, and CAR2 all show a faint but distinct DNA band above the highest rung on the DNA ladder (>10kb), showing the presence of DNA. No bands were seen on the kit flow-through. Indicates successful genomic extraction.  
-
 
+
*Preformed a second genomic extraction on <Picea abies <i/> to improve yield. Nanodrop shows CAR3 to be at 6.2 ng/ul and CAR4 to be 11.5 ng/ul.
 +
* Extracted genomic DNA from <i> Gossypim hirsutum </i>. CAD nanodropped to be 1.8 ng/ul and CAD2 7.8 ng/ul.
 +
<div style="color:black;font-size:11pt">'''April 1st'''</div>
 +
* Ran a gel on CAR3, CAR4, CAD1, and CAD2. Brighter genomic DNA bands were seen on the cadinene camples than before, but cadinene samples showed significant smearing near the top of the gel.
 +
<div style="color:black;font-size:11pt">'''April 1st'''</div>
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==<b><font size="6">Summer 2014</font></b>==
==<b><font size="6">Summer 2014</font></b>==
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Lab Notebook



Spring 2014

March 27th
  • Extracted genomic DNA from 100 mg of Arabidopsis thalina leaves using a Viogene DNA extraction kit. Samples were labeled ZIN for zingiberene.
  • Two samples were prepared and nanodropped. The concentration of the first was 2.6 ng/ul, and the second was 3.5 ng/ul of DNA, indicating a minimal yield of DNA.
March 30th
  • Extracted genomic DNA from 100 mg of Picea abies needles using the same extraction kit and protocol. Samples were labeled CAR for carene.
  • Two samples were prepared and nanodropped. The concentration of the first was 0.9 ng/ul, and the second was 3.5 ng/ul of DNA, indicating a minimal yield of DNA.
March 31th
  • Ran a 0.6 % argarose gel on the DNA extracted from ZIN and CAR, as well as the column flow-through from the kit.
  • ZIN1, ZIN2, CAR1, and CAR2 all show a faint but distinct DNA band above the highest rung on the DNA ladder (>10kb), showing the presence of DNA. No bands were seen on the kit flow-through. Indicates successful genomic extraction.
  • Preformed a second genomic extraction on <Picea abies <i/> to improve yield. Nanodrop shows CAR3 to be at 6.2 ng/ul and CAR4 to be 11.5 ng/ul.
  • Extracted genomic DNA from Gossypim hirsutum . CAD nanodropped to be 1.8 ng/ul and CAD2 7.8 ng/ul.
April 1st
  • Ran a gel on CAR3, CAR4, CAD1, and CAD2. Brighter genomic DNA bands were seen on the cadinene camples than before, but cadinene samples showed significant smearing near the top of the gel.
April 1st


Summer 2014

August 24th
  • Put text here


Fall 2014

August 24th
  • Put text here