WikitemplateB project

From 2014.igem.org

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Revision as of 19:32, 4 October 2014

REColi: Signal amplification and formattable bacterial memory by DNA edition.

What is our project at 2014? We have designed a synthetic digital device inspired by electronic circuits called multiplexers. Its 3-bit memory allows saving and processing binary inputs in E. coli. Induced expression of serine recombinases, capable of specific DNA editing, enables construction of biological analogues of transistors - transcriptors - and their use as elementary memory units called SR-latches. The fourth, strobe signal resets the system to its original state. We have shown that the system could efficiently store data about previous contact with inducing sugars across hundreds of generations. This memory unit offers high sensitivity in inducer detection and signal amplification, allowing cheap induction or further development and use as a trace contaminants sensor. Eight possible output combinations, reported as RGB fluorescent proteins, may also turn out to be useful for complex coexpression research.

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-<h3>One E.coli Has One Function
+<h3>REColi: Signal amplification and formattable bacterial memory by DNA edition.
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 What is our project at 2014?
-Cadmium is one of harmful materialｓ for us. 50 years ago, itai-iati disease was going around in center of Japan Gihu. The cause was industrial wastewater. Cadmium contained the water. Our project is to find a way to clean contaminated with Cadmium. we think how to clean the water. We use E.coli.
+We have designed a synthetic digital device inspired by electronic circuits called multiplexers. Its 3-bit memory allows saving and processing binary inputs in E. coli. Induced expression of serine recombinases, capable of specific DNA editing, enables construction of biological analogues of transistors - transcriptors - and their use as elementary memory units called SR-latches. The fourth, strobe signal resets the system to its original state. We have shown that the system could efficiently store data about previous contact with inducing sugars across hundreds of generations. This memory unit offers high sensitivity in inducer detection and signal amplification, allowing cheap induction or further development and use as a trace contaminants sensor. Eight possible output combinations, reported as RGB fluorescent proteins, may also turn out to be useful for complex coexpression research.
-Concretely, we use two kinds of E.coli. One catches Cadmium.　the Other makes to all E.coli to use chemoattractant. Catches E.coli displays metallothionein (metallothionein is a protein that combines a heavy metal. Cadomium is one kind of heavy metal). Other is releasing Asp (Asp is one kind of chemoattractant. All E.coli is close to the Asp E.coli).
-To use these E.coli. Finally cadomiun is catched(wastewater is be clean).
-What experiment we did?
-・How to check E.coli's chemotaxis?
-We make use of other igem team’s　assay.
-Using soft agar plate.we let E.coli siwm.
-・We made a plasmid
-Our project need to two kind of plasmid.
-we made one plasmid.And we have to make one more plasmid.
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