Team:ETH Zurich/lab/biobrick/used1

From 2014.igem.org

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== Characterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentration ==
+
= Characterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentration =
-
== Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSL ==
+
== Background information ==
-
=== System considered ===
+
=== Systems considered ===
-
=== Modeling leakiness ===
+
=== Modeling promoter's sensitivity ===
-
=== Results ===
+
== Results ==
-
== Characterization of two-level crosstalk on the promoter ==
+
= Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSL =
 +
 
 +
== Background information ==
=== Systems considered ===
=== Systems considered ===
 +
 +
=== Modeling leakiness ===
 +
 +
== Results ==
 +
 +
= Characterization of two-level crosstalk on the promoter =
 +
 +
== Background information ==
 +
 +
=== System considered ===
=== Modeling crosstalk ===
=== Modeling crosstalk ===
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=== First-level crosstalk: LuxR binds to different HSL and activates the promoter ===
+
== First-level crosstalk: LuxR binds to different HSL and activates the promoter ==
 +
 
 +
== Second-level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoter ==
 +
 
 +
=== Background information ===
-
=== Second-level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoter ===
+
=== Observations ===
-
=== Combination of both levels: other regulatory proteins, like LasR, bind to different HSL and activates the promoter ===
+
== Combination of both levels: other regulatory proteins, like LasR, bind to different HSL and activates the promoter ==
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=== Results ===
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== Results ==
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Revision as of 12:56, 3 October 2014

<partinfo>BBa_R0062 AddReview 4</partinfo> ETH Zurich 2014

Contents

Characterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentration

Background information

Systems considered

Modeling promoter's sensitivity

Results

Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSL

Background information

Systems considered

Modeling leakiness

Results

Characterization of two-level crosstalk on the promoter

Background information

System considered

Modeling crosstalk

First-level crosstalk: LuxR binds to different HSL and activates the promoter

Second-level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoter

Background information

Observations

Combination of both levels: other regulatory proteins, like LasR, bind to different HSL and activates the promoter

Results

;
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