Team:Freiburg/Content/Results/Vector

From 2014.igem.org

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<h1>1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; The Vector</h1>
<h1>1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; The Vector</h1>
<h2>1.1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Specificity of MuLV</h2>
<h2>1.1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Specificity of MuLV</h2>
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  <img src="https://2014.igem.org/File:Freiburg2014-09-10_vector-specificity_pic1.1.png">
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<div class="row category-row">
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        <p>An important aspect for the function of our system as well as for its safety is the specificity of the vector regarding infection of different
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kind of cells. The vector deriving from the murine leukemia virus is specific for cells carrying the mouse specific CAT-1. Cells that do not have
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this specific receptor are not targeted by the vector. In order to test the specificity of the system, different kind of cells were incubated with
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the vector..</p>
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      </div>
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      <div class="col-sm-6">
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        <figure>
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          <img src="https://static.igem.org/mediawiki/2014/f/fd/Freiburg2014-09-10_vector-specificity_pic1.1.png">
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          <figcaption>
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            <p class="header">Fig.X: Scheme for testing the specificity of the vector.</p>
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            <p class="desc">In order to test the specificity of the viral vector different cells were incubated with the vector for four hours. Cells not containing the mouse specific receptor
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CAT-1 are not capable for infection. As control mouse fibroblasts (NIH3t3) expressing CAT-1 were used.</p>
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          </figcaption>
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        </figure>
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      </div>
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    </div>
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<h2>1.2&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Optimization of transduction</h2>
<h2>1.2&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Optimization of transduction</h2>
<h2>1.3&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Expression of different viral constructs</h2>
<h2>1.3&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Expression of different viral constructs</h2>

Revision as of 21:43, 1 October 2014

The AcCELLerator

1          The Vector

1.1         Specificity of MuLV

An important aspect for the function of our system as well as for its safety is the specificity of the vector regarding infection of different kind of cells. The vector deriving from the murine leukemia virus is specific for cells carrying the mouse specific CAT-1. Cells that do not have this specific receptor are not targeted by the vector. In order to test the specificity of the system, different kind of cells were incubated with the vector..

Fig.X: Scheme for testing the specificity of the vector.

In order to test the specificity of the viral vector different cells were incubated with the vector for four hours. Cells not containing the mouse specific receptor CAT-1 are not capable for infection. As control mouse fibroblasts (NIH3t3) expressing CAT-1 were used.

1.2         Optimization of transduction

1.3         Expression of different viral constructs

1.4         Virus dilution and concentration

1.5         Half life time of MuLV

1.6         Integration time of MuLV

1.7         Stable integration

1.8         Stable Cell line with MuLV

2          The Receptor

2.1         Localisation Receptor

2.2         Expression of Receptor using different DNA concentrations

2.3         Receptor expression time

2.4         Receptor life time after splitting

3          The light system

3.1         Function of blue light system

3.2         Function of red light system

3.3         Light induced receptor/ blue light

3.4         Receptor functionality

3.5         SEAP as reporter

3.6         MuLV SEAP

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