Team:Freiburg/Content/Results/Vector

From 2014.igem.org

(Difference between revisions)
Line 8: Line 8:
<h1>1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; The Vector</h1>
<h1>1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; The Vector</h1>
<h2>1.1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Specificity of MuLV</h2>
<h2>1.1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Specificity of MuLV</h2>
 +
<h2>1.2&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Optimization of transduction</h2>
<h2>1.2&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Optimization of transduction</h2>
<h2>1.3&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Expression of different viral constructs</h2>
<h2>1.3&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Expression of different viral constructs</h2>

Revision as of 17:38, 1 October 2014

The AcCELLerator

1          The Vector

1.1         Specificity of MuLV

1.2         Optimization of transduction

1.3         Expression of different viral constructs

1.4         Virus dilution and concentration

1.5         Half life time of MuLV

1.6         Integration time of MuLV

1.7         Stable integration

1.8         Stable Cell line with MuLV

2          The Receptor

2.1         Localisation Receptor

2.2         Expression of Receptor using different DNA concentrations

2.3         Receptor expression time

2.4         Receptor life time after splitting

3          The light system

3.1         Function of blue light system

3.2         Function of red light system

3.3         Light induced receptor/ blue light

3.4         Receptor functionality

3.5         SEAP as reporter

3.6         MuLV SEAP

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