Team:TU Delft-Leiden/WetLab/landmine/characterisation
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- | As already <a href="/Team:TU_Delft-Leiden/WetLab/landmine">mentioned</a>, the promoters found to be activated in presence of several chemical compounds that can | + | As already <a href="/Team:TU_Delft-Leiden/WetLab/landmine">mentioned</a>, the promoters found to be activated in presence of several chemical compounds that can leak from land mines (ybiJ and yqjF) were coupled to the expression of the fluorescent protein mKate2. |
- | <h3> | + | <h3> Assays </h3> |
<p> | <p> | ||
- | The | + | The different assays used to test our Land Mine BioBricks are: |
+ | |||
+ | <li><a href="/Team:TU_Delft-Leiden/WetLab/landmine/characterisation#LDplate_reader"><p>Plate Reader </p> | ||
+ | </a> | ||
+ | </li> | ||
+ | |||
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+ | <li><a href="/Team:TU_Delft-Leiden/WetLab/landmine/characterisation#LDmicroscopy"><p>Microscopy </p> | ||
+ | </a> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li><a href="/Team:TU_Delft-Leiden/WetLab/landmine/characterisation#LDfacs"><p>FACS </p> | ||
+ | </a> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | <a name="LDplate_reader"></a> | ||
+ | <h3> Plate Reader </h3> | ||
+ | <p> | ||
+ | The plate reader is a machine that allows for the measuring of | ||
+ | |||
+ | |||
+ | <a name="LDmicroscopy"></a> | ||
+ | <h3> Microscopy </h3> | ||
+ | <p> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <a name="LDfacs"></a> | ||
+ | <h3> FACS </h3> | ||
+ | <p> | ||
+ | Fluorescence-activated cell sorting (FACS) is a specialised type of flow cytometry | ||
+ | |||
</html> | </html> | ||
{{:Team:TU_Delft-Leiden/Templates/End}} | {{:Team:TU_Delft-Leiden/Templates/End}} |
Revision as of 16:52, 30 September 2014
Landmine Module - Characterisation
As already mentioned, the promoters found to be activated in presence of several chemical compounds that can leak from land mines (ybiJ and yqjF) were coupled to the expression of the fluorescent protein mKate2.
Assays
The different assays used to test our Land Mine BioBricks are:
Plate Reader
Microscopy
FACS
Plate Reader
The plate reader is a machine that allows for the measuring of
Microscopy
FACS
Fluorescence-activated cell sorting (FACS) is a specialised type of flow cytometry