"
Team:TU Delft-Leiden/WetLab/landmine/cloning/yqjF
From 2014.igem.org
(Difference between revisions)
Joanctd.igem (Talk | contribs) |
Joanctd.igem (Talk | contribs) |
||
| Line 11: | Line 11: | ||
<p> | <p> | ||
| - | + | Two final constructs are based on the yqjF promoter alone: | |
| - | < | + | <a href="http://parts.igem.org/Part:BBa_K1316003" style="text-decoration: none"" target="_blank"><font color="#0080FF" size="3">BBa_K1316003</font></a> |
| - | + | and | |
| + | <a href="http://parts.igem.org/Part:BBa_K1316003" style="text-decoration: none"" target="_blank"><font color="#0080FF" size="3">BBa_K1316005</font></a>. For both of them the reporter gene mKate2 was placed behind the yqjF promoter. As BBa_K1316005 was built on bases of BBa_K1316003, a double transcriptional terminator was placed after the mKate2 gene. Otherwise, the RNA polymerase would continue transcribing and some unfinised transcripts of the N-genes could interfere with the normal expression of these genes. Therefore, mKate2 gene was first introduced in front of a Double terminator ( | ||
| + | <a href="http://parts.igem.org/Part:BBa_K823017" style="text-decoration: none"" target="_blank"><font color="#0080FF" size="3">BBa_K823017</font></a> | ||
| + | ) | ||
</p> | </p> | ||
<p> | <p> | ||
| - | + | <img src="https://static.igem.org/mediawiki/2014/9/96/TUDelft_Land_Mine_pF_Final_LandMines_History.png" width="100%" height="100%"> | |
</p> | </p> | ||
| - | |||
</html> | </html> | ||
{{:Team:TU_Delft-Leiden/Templates/End}} | {{:Team:TU_Delft-Leiden/Templates/End}} | ||
Revision as of 13:20, 30 September 2014
Landmine Module - Cloning - yqjF promoter
yqjF promoter
Two final constructs are based on the yqjF promoter alone: BBa_K1316003 and BBa_K1316005. For both of them the reporter gene mKate2 was placed behind the yqjF promoter. As BBa_K1316005 was built on bases of BBa_K1316003, a double transcriptional terminator was placed after the mKate2 gene. Otherwise, the RNA polymerase would continue transcribing and some unfinised transcripts of the N-genes could interfere with the normal expression of these genes. Therefore, mKate2 gene was first introduced in front of a Double terminator ( BBa_K823017 )
