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Team:TU Delft-Leiden/Modeling/EET
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| - | In the wet lab, we integrated the extracellular electron transport (EET) module of <i>S. oneidensis</i> into <i> E. coli </i><font color="red">[reference]</font>. For the modeling of the EET module, we wanted at first to gain insight in the consequences of the integration of the EET module into <i>E. coli</i>. To achieve this, we had to find a way to simulate the cell metabolism of <i>E. coli</i> including the EET module. Flux Balance Analysis (FBA) is a method that calculates the fluxes of metabolites through a metabolic network [1], more about the theory of Flux Balance Analysis can be found in <a href="https://2014.igem.org/Team:TU_Delft-Leiden/Modeling/Techniques/FBA">Flux Balance Analysis Theory</a>. Therefore, we decided to use the FBA method to gain insight in the resulting cell metabolism of <i>E. coli</i>. Our goal was to investigate the carbon metabolism providing the electrons for the EET module <font color="red">[reference]</font>. Also, we want the EET pathway used by the cells in order to have a measurable electrical signal for our biosensor <font color="red">[reference]</font>. | + | In the wet lab, we integrated the extracellular electron transport (EET) module of <i>S. oneidensis</i> into <i> E. coli </i><font color="red">[reference]</font>. For the modeling of the EET module, we wanted at first to gain insight in the consequences of the integration of the EET module into <i>E. coli</i>. To achieve this, we had to find a way to simulate the cell metabolism of <i>E. coli</i> including the EET module. Flux Balance Analysis (FBA) is a method that calculates the fluxes of metabolites through a metabolic network [1], more about the theory of Flux Balance Analysis can be found in <a href="https://2014.igem.org/Team:TU_Delft-Leiden/Modeling/Techniques/FBA">Flux Balance Analysis Theory</a>. Therefore, we decided to use the FBA method to gain insight in the resulting cell metabolism of <i>E. coli</i>. Our goal was to investigate the carbon metabolism providing the electrons for the EET module <font color="red">[reference]</font>. Also, we want the EET pathway used by the cells in order to have a measurable electrical signal for our biosensor <font color="red">[reference]</font>. The results of the Flux Balance Analysis can be found <a href="https://2014.igem.org/Team:TU_Delft-Leiden/Modeling/EET/FBA">here</a> |
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Revision as of 14:36, 28 September 2014
EET Module
In the wet lab, we integrated the extracellular electron transport (EET) module of S. oneidensis into E. coli [reference]. For the modeling of the EET module, we wanted at first to gain insight in the consequences of the integration of the EET module into E. coli. To achieve this, we had to find a way to simulate the cell metabolism of E. coli including the EET module. Flux Balance Analysis (FBA) is a method that calculates the fluxes of metabolites through a metabolic network [1], more about the theory of Flux Balance Analysis can be found in Flux Balance Analysis Theory. Therefore, we decided to use the FBA method to gain insight in the resulting cell metabolism of E. coli. Our goal was to investigate the carbon metabolism providing the electrons for the EET module [reference]. Also, we want the EET pathway used by the cells in order to have a measurable electrical signal for our biosensor [reference]. The results of the Flux Balance Analysis can be found here
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