Team:Carnegie Mellon/InterLab

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                     <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Weeks">Week by Week</a></li>
                     <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Weeks">Week by Week</a></li>
                     <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Sensor">Sensor</a></li>
                     <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Sensor">Sensor</a></li>
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                    <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/InterLab">Inter Lab Study</a></li>
 
                     <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Protein">Fluorescent Protein Evaluation</a></li>
                     <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Protein">Fluorescent Protein Evaluation</a></li>
                     <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Superoxide">Superoxide Generator Improvement</a></li>
                     <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/Superoxide">Superoxide Generator Improvement</a></li>
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                    <li><a href="https://2014.igem.org/Team:Carnegie_Mellon/InterLab">Interlab Study</a></li>
                   </ul>
                   </ul>
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Revision as of 17:10, 26 September 2014

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Interlab Study

Goal: To collect fluorescence data from three devices from different teams around the world

Plasmids

Plasmids

Description: We constructed these devices by digesting these three plasmids, the J23101 promoter, the J23115 promoter and the E0240 (GFP), with restriction enzymes XbaI and SpeI. We then ligated the GFP fragment into the plasmids containing the promoters. We then transformed these plasmids back into E. coli MACH cells to screen the cells for fluorescence. After identifying the cells containing both the promoter and the GFP, we streaked these colonies out and then repeated the selection for cells containing GFP. The DNA from overnight cultures made of these cells was extracted, then transformed these two plasmas, and the third pSB3K3 plasmid (containing the J23101 promoter and GFP) into competent Top10 E. coli cells. We then measured fluorescence from these samples in triplicate (along with non-expressing control cells), using the TECAN, to get fluorescence values in RFU (relative fluorescent units).

Week by Week Notebook Entries

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