Team:UFAM Brazil/7-17-2014
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Revision as of 19:38, 25 September 2014
07/17/2014 | ||
Today we minipreped DH5α with BBa_E0840 (Biodetection Biobrick) and DH5α with BBa_K346004 (Bioaccumulation Biobrick). Eletrophoretical profile from the samples: | ||
We concentrated the extracted biobricks to transform in JM110. After the plasmid concentration, the JM110 got chemically competent with a rapid method with Sorbitol 1M and the electroporation with: - Negative control, no DNA. - Positive control with pUC72; - BBa_E0840 (Biodetection Biobrick); - BBa_K346004 (Bioaccumulation Biobrick). | ||
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