Team:Uppsala/Project Notebook

From 2014.igem.org

(Difference between revisions)
Line 69: Line 69:
],["<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-BFP</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>lux pL-B0034-luxR-lux pR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>lux pR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-YenR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>B0034</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>psB1A3</td><td>CFY(Bacteriocin)</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>psB1A3</td><td>CFY(Bacteriocin)</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>Plasmid prep</td><td>J.M</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>Screening</td><td>J.M</td></tr>","<tr><td>dH5-alpha</td><td>pSB1K3</td><td>B0034-CheZ</td><td>2% agarose gel to assess restriction success</td><td>D.B. E.Q.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>",
],["<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-BFP</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>lux pL-B0034-luxR-lux pR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>lux pR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-YenR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>B0034</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>psB1A3</td><td>CFY(Bacteriocin)</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>psB1A3</td><td>CFY(Bacteriocin)</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>Plasmid prep</td><td>J.M</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>Screening</td><td>J.M</td></tr>","<tr><td>dH5-alpha</td><td>pSB1K3</td><td>B0034-CheZ</td><td>2% agarose gel to assess restriction success</td><td>D.B. E.Q.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>",
],["<tr><td></td><td></td><td></td><td>Tryptone swarm plates</td><td>M.S. D.B.</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>CheZ</td><td>mutagenic PCR (SynBio protocol, ext. time = 60 sec)</td><td>E.Q.</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>CheZ</td><td>mutagenic PCR (SynBio protocol, ext. time = 90 sec)</td><td>E.Q.</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>YenBox_wt + pSB1C3</td><td>Ligation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>YenBox_J23113 + pSB1C3</td><td>Ligation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>YenBox_wt + B0034-BFP + pSB1A3</td><td>Ligation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>YenBox_J23113 + B0034-BFP + pSB1A3</td><td>Ligation</td><td>M.F/C.A</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>transformation (from kit)</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>LuxpR-B0034-BFP</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>lux pL-B0034-luxR-lux pR - BFP</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-YenR</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-YenR</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>RBS-LacZ alpha fragment</td><td>transformation (from kit)</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>RBS-LacZ</td><td>transformation (from kit)</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>red</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>GFP fusion brick</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23101</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A res</td><td>J23112-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23113-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23109</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23114-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23105-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23106-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23100-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA (bad suffix)</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA (bad suffix)</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-PelB</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-USP45-CFY (bad suffix)</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>CFY</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>CFY</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pelB-CFY (Bad Suffix)</td><td>pSB3T5</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pelB-CFY (Bad Suffix)</td><td>pSB3T5</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA-GFP (bad suffix)</td><td>pSB3T5</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA-GFP (bad suffix)</td><td>pSB3T5</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA-CFY (bad suffix)</td><td>pSB3T5</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA-CFY (bad suffix)</td><td>pSB3T5</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA</td><td>pSB1C3</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA</td><td>pSB1C3</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23102</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23102</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23106</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23106</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-CheZ</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-CheZ</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td></td><td></td><td></td><td></td><td></td></tr>",
],["<tr><td></td><td></td><td></td><td>Tryptone swarm plates</td><td>M.S. D.B.</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>CheZ</td><td>mutagenic PCR (SynBio protocol, ext. time = 60 sec)</td><td>E.Q.</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>CheZ</td><td>mutagenic PCR (SynBio protocol, ext. time = 90 sec)</td><td>E.Q.</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>YenBox_wt + pSB1C3</td><td>Ligation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>YenBox_J23113 + pSB1C3</td><td>Ligation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>YenBox_wt + B0034-BFP + pSB1A3</td><td>Ligation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>YenBox_J23113 + B0034-BFP + pSB1A3</td><td>Ligation</td><td>M.F/C.A</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>transformation (from kit)</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>LuxpR-B0034-BFP</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>lux pL-B0034-luxR-lux pR - BFP</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-YenR</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-YenR</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>RBS-LacZ alpha fragment</td><td>transformation (from kit)</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>RBS-LacZ</td><td>transformation (from kit)</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>red</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>GFP fusion brick</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23101</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A res</td><td>J23112-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23113-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23109</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23114-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23105-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23106-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>A-res</td><td>J23100-red</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA (bad suffix)</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>B0034-pgsA (bad suffix)</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-PelB</td><td>digest</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-USP45-CFY (bad suffix)</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>CFY</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>CFY</td><td>transformation</td><td>A.G L.P</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pJP059 (C and E resistance)</td><td>red</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pelB-CFY (Bad Suffix)</td><td>pSB3T5</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pelB-CFY (Bad Suffix)</td><td>pSB3T5</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA-GFP (bad suffix)</td><td>pSB3T5</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA-GFP (bad suffix)</td><td>pSB3T5</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA-CFY (bad suffix)</td><td>pSB3T5</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA-CFY (bad suffix)</td><td>pSB3T5</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA</td><td>pSB1C3</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-pgsA</td><td>pSB1C3</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23102</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23102</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23106</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23106</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23113</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>J23114</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-CheZ</td><td>ligation</td><td>T.H.E M.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>B0034-CheZ</td><td>transformation</td><td>T.H.E M.H</td></tr>","<tr><td></td><td></td><td></td><td></td><td></td></tr>",
-
],[2],[3],[4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5],
+
],["<tr><td>D5-alfa</td><td>pSB1C3</td><td>YenBox_wt</td><td>Transformation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>YenBox_J23113</td><td>Transformation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>YenBox_wt-B0034-BFP</td><td>Transformation</td><td>M.F/C.A</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>YenBox_J23113-B0034-BFP</td><td>Transformation</td><td>M.F/C.A</td></tr>","<tr><td>DH5-alpha</td><td>pSB1C3</td><td>CheZ</td><td>mutPCR (SynBio protocol, ext. time = 90 sec), second try</td><td>E.Q.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>O/N culture</td><td>M.S. D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>O/N culture</td><td>M.S. D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>O/N culture</td><td>M.S. D.B.</td></tr>","<tr><td></td><td></td><td></td><td>Plates</td><td>C.A M.H M.F A.G</td></tr>","<tr><td></td><td></td><td></td><td>LB agar</td><td>M.H</td></tr>",
 +
],[3],[4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5],
];
];
</script>
</script>

Revision as of 18:54, 24 September 2014

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