Team:Uppsala/Project Notebook

From 2014.igem.org

(Difference between revisions)
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],["<tr><td>RP437</td><td></td><td></td><td>restreak</td><td>D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>restreak</td><td>D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>restreak</td><td>D.B.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>motility test on LA, 5 hours</td><td>D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>motility test on LA, 5 hours</td><td>D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>motility test on LA, 5 hours</td><td>D.B.</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pBAD</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pBAD</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>ON</td><td>G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>ON</td><td>G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>ON</td><td>G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pBAD</td><td>ON</td><td>G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Restreak</td><td>M.F</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>digest</td><td>M.H</td></tr>","<tr><td>D5-alfa</td><td>pUCIDT (Kan)</td><td>Colicin fyA (bacteriocin)</td><td>digest</td><td>M.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pelB</td><td>digest</td><td>M.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>USP45</td><td>ligering</td><td>M.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>CFY(Bacteriocin)</td><td>ligering</td><td>M.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1K3</td><td>B0034-PelB</td><td>ligering</td><td>M.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>CFY(Bacteriocin)</td><td>transformering</td><td>N.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>USP45</td><td>transformering</td><td>N.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1K3</td><td>B0034-PelB</td><td>transformering</td><td>N.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>red</td><td>digest</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>red</td><td>digest</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pJP059</td><td>Shuttlevector</td><td>digest</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-PgsA</td><td>B0034-PgsA</td><td>digest</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>PsgA</td><td>ligation</td><td>J.M M.H N.H</td></tr>",],["<tr><td>D5-alpha</td><td>pSB1C3</td><td>USP45</td><td>Overnight</td><td>V.B</td></tr>","<tr><td>D5-alpha</td><td>pSB1C3</td><td>LuxR</td><td>Overnight</td><td>V.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Plasmid prep</td><td>M.F</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Plasmid prep</td><td>M.F</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Plasmid prep</td><td>M.F</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pBAD</td><td>Plasmid prep</td><td>M.F</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>ON</td><td>V.B</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>Restreak</td><td>J.M</td></tr>",
],["<tr><td>RP437</td><td></td><td></td><td>restreak</td><td>D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>restreak</td><td>D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>restreak</td><td>D.B.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>motility test on LA, 5 hours</td><td>D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>motility test on LA, 5 hours</td><td>D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>motility test on LA, 5 hours</td><td>D.B.</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pBAD</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pBAD</td><td>Screening</td><td>M.F/G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>ON</td><td>G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>ON</td><td>G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>ON</td><td>G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pBAD</td><td>ON</td><td>G.C</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Restreak</td><td>M.F</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>digest</td><td>M.H</td></tr>","<tr><td>D5-alfa</td><td>pUCIDT (Kan)</td><td>Colicin fyA (bacteriocin)</td><td>digest</td><td>M.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pelB</td><td>digest</td><td>M.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>USP45</td><td>ligering</td><td>M.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>CFY(Bacteriocin)</td><td>ligering</td><td>M.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1K3</td><td>B0034-PelB</td><td>ligering</td><td>M.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>CFY(Bacteriocin)</td><td>transformering</td><td>N.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1A3</td><td>USP45</td><td>transformering</td><td>N.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1K3</td><td>B0034-PelB</td><td>transformering</td><td>N.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>red</td><td>digest</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>red</td><td>digest</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pJP059</td><td>Shuttlevector</td><td>digest</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>B0034-PgsA</td><td>B0034-PgsA</td><td>digest</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>PsgA</td><td>ligation</td><td>J.M M.H N.H</td></tr>",],["<tr><td>D5-alpha</td><td>pSB1C3</td><td>USP45</td><td>Overnight</td><td>V.B</td></tr>","<tr><td>D5-alpha</td><td>pSB1C3</td><td>LuxR</td><td>Overnight</td><td>V.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Plasmid prep</td><td>M.F</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Plasmid prep</td><td>M.F</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Plasmid prep</td><td>M.F</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pBAD</td><td>Plasmid prep</td><td>M.F</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>ON</td><td>V.B</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>Restreak</td><td>J.M</td></tr>",
],["<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>ON</td><td>J.M</td></tr>",
],["<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>ON</td><td>J.M</td></tr>",
-
],[30],[1],[2],[3],[4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5],
+
],["<tr><td>DH5-alfa</td><td>pSB1C3</td><td>B0034-BFP</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>lux pL-B0034-luxR-lux pR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>lux pR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-YenR</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB3T5</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A3</td><td>red</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>B0034</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>psB1A3</td><td>CFY(Bacteriocin)</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>DH5-alfa</td><td>psB1A3</td><td>CFY(Bacteriocin)</td><td>digest</td><td>V.B J.M A.G</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>Plasmid prep</td><td>J.M</td></tr>","<tr><td>D5-alfa</td><td>pIDTSmart (Kan)</td><td>YenR</td><td>Screening</td><td>J.M</td></tr>","<tr><td>dH5-alpha</td><td>pSB1K3</td><td>B0034-CheZ</td><td>2% agarose gel to assess restriction success</td><td>D.B. E.Q.</td></tr>","<tr><td>RP437</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>","<tr><td>RP1616</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>","<tr><td>UU2685</td><td></td><td></td><td>motility test on LA, longer time</td><td>D.B.</td></tr>",
 +
],[1],[2],[3],[4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5],[6],[7],[8],[9],[10],[11],[12],[13],[14],[15],[16],[17],[18],[19],[20],[21],[22],[23],[24],[25],[26],[27],[28],[29],[30],[31],[1],[2],[3],[4],[5],
];
];
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Revision as of 18:51, 24 September 2014

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