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Team:Uppsala/Project Notebook
From 2014.igem.org
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["<tr><td>DH5-alfa </td><td>PSB1A3</td><td>red</td><td>plasmid prep</td><td>J.M </td></tr>","<tr><td>DH5-alfa </td><td>pSB1C3</td><td>spot42</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>", "<tr><td>DH5-alfa </td><td>pSB1C3</td><td>PelB</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>pSB1C3</td><td>BBa_K592024</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>BBa_J61002</td><td>BBa_J23101</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>BBa_J61002</td><td>BBa_J23110</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>PJP059</td><td>shuttle vector</td><td>plasmid prep</td><td>O.P</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23102</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>pSB1A2</td><td>GFP fusion brick</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>BBa_K823005</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>BBa_J61002</td><td>BBa_J23112</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23113</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>BBa_J23109</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>BBa_J61002</td><td> BBa_J23114</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa</td><td> BBa_J61002</td><td> BBa_J23105</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td> BBa_J61002</td><td> BBa_J23106</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa</td><td> BBa_J61002</td><td> BBa_J23100</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td> pSB1A2</td><td>BBa_B0034</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>pSB1C3</td><td>BBa_K629003</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>"], | ["<tr><td>DH5-alfa </td><td>PSB1A3</td><td>red</td><td>plasmid prep</td><td>J.M </td></tr>","<tr><td>DH5-alfa </td><td>pSB1C3</td><td>spot42</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>", "<tr><td>DH5-alfa </td><td>pSB1C3</td><td>PelB</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>pSB1C3</td><td>BBa_K592024</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>BBa_J61002</td><td>BBa_J23101</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>BBa_J61002</td><td>BBa_J23110</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>PJP059</td><td>shuttle vector</td><td>plasmid prep</td><td>O.P</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23102</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>pSB1A2</td><td>GFP fusion brick</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>BBa_K823005</td><td>restreak</td><td>C.A/M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>BBa_J61002</td><td>BBa_J23112</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23113</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>BBa_J23109</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>BBa_J61002</td><td> BBa_J23114</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa</td><td> BBa_J61002</td><td> BBa_J23105</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td> BBa_J61002</td><td> BBa_J23106</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa</td><td> BBa_J61002</td><td> BBa_J23100</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td> pSB1A2</td><td>BBa_B0034</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa </td><td>pSB1C3</td><td>BBa_K629003</td><td>transformation (from kit)</td><td>M.F/G.C/M.B</td></tr>"], | ||
| - | ["<tr><td>DH5-alfa</td><td>pSB1C3</td><td>spot42</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>PelB</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>BBa_K592024</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23101</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23110</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23102</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>GFP fusion brick</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>BBa_K823005</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23112</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23113</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>BBa_J23109</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23114</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23105</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23106</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23100</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>BBa_B0034</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>BBa_K629003</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>"],[2100],[2200],["<tr><td></td><td></td><td></td><td>Tetracyclin</td><td>CA/MF/MB</td></tr>"],["<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>restreak</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pUCIDT (Kan)</td><td>Colicin fyA (bacteriocin)</td><td>restreak</td><td>J.M</td></tr>","<tr><td>DH5-alfa</td><td>pUCIDT (Kan)</td><td>PsgA</td><td>restreak</td><td>G.C</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>Colagen</td><td>restreak</td><td>A.G</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>O/N</td><td>J.M M.H N.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Transformation from kit</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Transformation from kit</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Transformation from kit</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Transformation from kit</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pBAD</td><td>Transformation from kit</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>red</td><td>digest</td><td>K.K. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>B0034</td><td>digest</td><td>K.K. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>cheZ</td><td>digest</td><td>K.K. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>ligation</td><td>K.K. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>transformation</td><td>K.K. D.B.</td></tr>","<tr><td></td><td></td><td></td><td>LB Agar</td><td></td></tr>",],[ | + | ["<tr><td>DH5-alfa</td><td>pSB1C3</td><td>spot42</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>PelB</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>BBa_K592024</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23101</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23110</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23102</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>GFP fusion brick</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>BBa_K823005</td><td>O/N</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23112</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23113</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>BBa_J23109</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23114</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23105</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23106</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>BBa_J61002</td><td>BBa_J23100</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>BBa_B0034</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>BBa_K629003</td><td>restreak</td><td>N.H/A.V/V.B/M.F</td></tr>"],[2100],[2200],["<tr><td></td><td></td><td></td><td>Tetracyclin</td><td>CA/MF/MB</td></tr>"],["<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>restreak</td><td>M.H</td></tr>","<tr><td>DH5-alfa</td><td>pUCIDT (Kan)</td><td>Colicin fyA (bacteriocin)</td><td>restreak</td><td>J.M</td></tr>","<tr><td>DH5-alfa</td><td>pUCIDT (Kan)</td><td>PsgA</td><td>restreak</td><td>G.C</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>Colagen</td><td>restreak</td><td>A.G</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>O/N</td><td>J.M M.H N.H</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Transformation from kit</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Transformation from kit</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Transformation from kit</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Transformation from kit</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pBAD</td><td>Transformation from kit</td><td>M.F/G.C/M.B</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>red</td><td>digest</td><td>K.K. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1A2</td><td>B0034</td><td>digest</td><td>K.K. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1C3</td><td>cheZ</td><td>digest</td><td>K.K. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>ligation</td><td>K.K. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>transformation</td><td>K.K. D.B.</td></tr>","<tr><td></td><td></td><td></td><td>LB Agar</td><td></td></tr>",],["<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>ligation with Taq</td><td>K.K. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>ligation with mystery ligase</td><td>K.K. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>ligation with T4</td><td>K.K. D.B.</td></tr>","<tr><td>DH5-alfa</td><td>pSB1K3</td><td>B0034-CheZ</td><td>transformation</td><td>K.K. D.B.</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>pBAD</td><td>Transformation from kit</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpL-B0034-LuxR-LuxpR</td><td>Restreak</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxI</td><td>Restreak</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxR</td><td>Restreak</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alfa</td><td>pSB1C3</td><td>LuxpR</td><td>Restreak</td><td>M.F/G.C/M.B</td></tr>","<tr><td>D5-alpha</td><td>pSB1C3</td><td>PsB1C3-red</td><td>Digestion</td><td>T.H</td></tr>","<tr><td>D5-alpha</td><td>pSB1C3</td><td>PsBIA3-red</td><td>Digestion</td><td>T.H</td></tr>","<tr><td>D5-alpha</td><td>pIDTSmart (Kan)</td><td>USP45</td><td>Digestion</td><td>T.H</td></tr>","<tr><td>D5-alpha</td><td>pUCIDT (Kan)</td><td>Bacteriocin</td><td>Digestion</td><td>T.H</td></tr>","<tr><td>D5-alpha</td><td>pUCIDT (Kan)</td><td>PgsA</td><td>Digestion</td><td>T.H</td></tr>","<tr><td>D5-alpha</td><td>pSB1C3</td><td>PsB1C3-red</td><td>Ligation</td><td>T.H</td></tr>","<tr><td>D5-alpha</td><td>pSB1C3</td><td>PsBIA3-red</td><td>Ligation</td><td>T.H</td></tr>","<tr><td>D5-alpha</td><td>pIDTSmart (Kan)</td><td>USP45</td><td>Ligation</td><td>T.H</td></tr>","<tr><td>D5-alpha</td><td>pUCIDT (Kan)</td><td>Bacteriocin</td><td>Ligation</td><td>T.H</td></tr>","<tr><td>D5-alpha</td><td>pUCIDT (Kan)</td><td>PgsA</td><td>Ligation</td><td>T.H</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>O/N</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>B0034-USP45</td><td>Screening</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pUCIDT (Kan)</td><td>Colicin fyA (bacteriocin)</td><td>O/N</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pUCIDT (Kan)</td><td>Colicin fyA (bacteriocin)</td><td>Screening</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pUCIDT (Kan)</td><td>PsgA</td><td>O/N</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pUCIDT (Kan)</td><td>PsgA</td><td>Screening</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>Colagen</td><td>O/N</td><td>J.M M.H N.H</td></tr>","<tr><td>DH5-alfa</td><td>pIDTSmart (Kan)</td><td>Colagen</td><td>Screening</td><td>J.M M.H N.H</td></tr>", |
| + | ],[26],[27], | ||
]; | ]; | ||
</script> | </script> | ||
Revision as of 18:38, 24 September 2014
Stephanie Herman
Teresa Reinli
Joakim Hellner
Alexander Virtanen
Jennifer Rosenius
Marcus Hong
Miranda Stiernborg
Tim Hagelby Edström
Viktor Blomkvist
Megha Biradar
Niklas Handin
Jonas Mattisson
Arina Gromov
Nils Anlind
Eric Sandström
Gunta Celma
Oliver Possnert
Martin Friberg
Kira Karlsson
Christoffer Andersson
Laura Pacoste
Andries Willem Boers
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