Team:ITESM-CEM/Attributions

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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    <td colspan="3" valign="bottom"><h3>ITESM-CEM | Medical Bioremediation</h3></td>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:ITESM-CEM/Attributions&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:ITESM-CEM/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=ITESM-CEM"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:ITESM-CEM/Attributions"style="color:#000000"> Attributions </a></td>
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    <td colspan="3" rowspan="2"><h1>Attributions
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        <h1n> 4205</h1n></h1></td>
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">Interlab</a></sub>
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">Existing GFP device</a></sub>
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">New GFP device 1</a></sub>
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      <sub><a href="https://2014.igem.org/Team:ITESM-CEM/Interlab">New GFP device 2</a></sub>
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<tr><td > <h3> iGEM Team attributions page</h3></td>
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Each team must clearly attribute work done by the student team members on this page.  The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster.
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We have this requirement to help the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, just be sure to report the work your team did and the work that was done by others.
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is something that is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.  
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<h2>Overview</h2>
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      <p>During 2014 iGEM competition, teams were requested to analyse the efficiency of 3 different genetic devices (BioBricks) using GFP as a marker of gene expression. Here, iGEM ITESM CEM team presents the results of this interlab fluorescence measurement study.
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The three devices analysed are composed by a variable promoter, a gene encoding a mutant Green Fluorescence Protein (GFP) used as a marker of expression, and a plasmid backbone. Two promoters (BBa_J23101 and BBa_J23115, recently renamed BBa_K823005 and BBa_K823012 at iGEM’s catalogue) are used, both of them being members of a family of constitutive promoters described by Chris Anderson, member of iGEM Berkley Team, in 2006 (1). This family of parts is registered at the catalogue under the alphanumeric codes BBa_J23100 – BBa_J23119.
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A great example of complete attribution comes from the <a href="https://2011.igem.org/Team:Imperial_College_London/Team">Imperial College London 2011 team</a> (scroll down to the bottom of their team page to see attributions).  
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Two different plasmid backbones are used: a low-copy (psB3K3) and a high-copy plasmid (psB1C3). The GFP-expressing BioBrick remains the same for all devices (registered at the catalog as BBa_E0240), and is composed of a ribosome binding site (RBS), a mutant GFP gene, and two termination sequences.
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The aim of this study is to report the relative efficiency of the following genetic devices:<br><br>
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1) Promoter BBa_K823005 in low-copy plasmid psB3K3<br>
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2) Promoter BBa_K823005 in high-copy plasmig psB1C3<br>
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3) Promoter BBa_K823012 in high-copy plasmid psB1C3<br><br>
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In order to do so, GFP (BBa_E0240) is used as a marker of gene expression or reporter gene, because of the ease of fluorescence measurement experiments.<br><br>
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GFP has long been used as a reporter of patterns of gene expression in both prokaryotes, were it is useful for characterization of promoters, enhancers and terminators; and in eukaryotes, were tissue-specific or time-specific gene expression can be traced (2). The basis of this procedure is the usage of GFP’s fluorescence as a reporter of activity of promoters and enhancers; the relative fluorescence of cells at different experimental conditions can be compared with statistical techniques, and so the efficiency of the parts can be tested.
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Here are some of the fields we recommend you have on this page. If there are other areas not listed below, but applicable to your team/project, please feel free to also list them on your attributions page. Please feel free to remove any areas not applicable to your project.
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<li>General Support</li>
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Revision as of 18:08, 24 September 2014

TEC-CEM | Attributions

ITESM-CEM | Medical Bioremediation

Attributions 4205
 

Overview

During 2014 iGEM competition, teams were requested to analyse the efficiency of 3 different genetic devices (BioBricks) using GFP as a marker of gene expression. Here, iGEM ITESM CEM team presents the results of this interlab fluorescence measurement study.

The three devices analysed are composed by a variable promoter, a gene encoding a mutant Green Fluorescence Protein (GFP) used as a marker of expression, and a plasmid backbone. Two promoters (BBa_J23101 and BBa_J23115, recently renamed BBa_K823005 and BBa_K823012 at iGEM’s catalogue) are used, both of them being members of a family of constitutive promoters described by Chris Anderson, member of iGEM Berkley Team, in 2006 (1). This family of parts is registered at the catalogue under the alphanumeric codes BBa_J23100 – BBa_J23119.

Two different plasmid backbones are used: a low-copy (psB3K3) and a high-copy plasmid (psB1C3). The GFP-expressing BioBrick remains the same for all devices (registered at the catalog as BBa_E0240), and is composed of a ribosome binding site (RBS), a mutant GFP gene, and two termination sequences.

The aim of this study is to report the relative efficiency of the following genetic devices:

1) Promoter BBa_K823005 in low-copy plasmid psB3K3
2) Promoter BBa_K823005 in high-copy plasmig psB1C3
3) Promoter BBa_K823012 in high-copy plasmid psB1C3

In order to do so, GFP (BBa_E0240) is used as a marker of gene expression or reporter gene, because of the ease of fluorescence measurement experiments.

GFP has long been used as a reporter of patterns of gene expression in both prokaryotes, were it is useful for characterization of promoters, enhancers and terminators; and in eukaryotes, were tissue-specific or time-specific gene expression can be traced (2). The basis of this procedure is the usage of GFP’s fluorescence as a reporter of activity of promoters and enhancers; the relative fluorescence of cells at different experimental conditions can be compared with statistical techniques, and so the efficiency of the parts can be tested.







 

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