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Team:DTU-Denmark
From 2014.igem.org
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<h1>Our Project</h1><br> | <h1>Our Project</h1><br> | ||
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<regulartext>Synthetic biology is the future approach for creating biological systems with novel functions. Doing this requires a large toolbox of genetic parts, having precisely described functions. <br> | <regulartext>Synthetic biology is the future approach for creating biological systems with novel functions. Doing this requires a large toolbox of genetic parts, having precisely described functions. <br> | ||
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| - | At the DTU iGEM team 2014, we have found that characterization of one of the most central tools is missing - genetic promoters are largely uncharacterised. Because of their importance in all synthetic biology systems, it is vital that promoter strength can be characterized in detail. | + | At the DTU iGEM team 2014, we have found that characterization of one of the most central tools is missing - genetic promoters are largely uncharacterised. Because of their importance in all synthetic biology systems, it is vital that promoter strength can be characterized in detail. <br> |
During our project we seek to develop a convenient, fast and reliable method for measuring absolute promoter activities using the Spinach technology. This method relies on fluorescently tagging RNA molecules called “Spinach”. By means of the Spinach concept, we will measure the concentration of the RNA product from different promoters and use this to calculate the transcription rate of the promoters. Using standard references in our measurements will even allow us to calculate transcription rates in units of Polymerases Per Second (PoPS), which is regarded as the optimal promoter activity measure.<br></regulartext> | During our project we seek to develop a convenient, fast and reliable method for measuring absolute promoter activities using the Spinach technology. This method relies on fluorescently tagging RNA molecules called “Spinach”. By means of the Spinach concept, we will measure the concentration of the RNA product from different promoters and use this to calculate the transcription rate of the promoters. Using standard references in our measurements will even allow us to calculate transcription rates in units of Polymerases Per Second (PoPS), which is regarded as the optimal promoter activity measure.<br></regulartext> | ||
Revision as of 17:45, 24 September 2014
- Home
- Overview
- Methods
- Achievements
- Team
At the Giant Jamboree, we received a gold medal and an Interlab Study Award. We also won the Overgrad Best Parts Collection.
Welcome
Our Project
At the DTU iGEM team 2014, we have found that characterization of one of the most central tools is missing - genetic promoters are largely uncharacterised. Because of their importance in all synthetic biology systems, it is vital that promoter strength can be characterized in detail.
During our project we seek to develop a convenient, fast and reliable method for measuring absolute promoter activities using the Spinach technology. This method relies on fluorescently tagging RNA molecules called “Spinach”. By means of the Spinach concept, we will measure the concentration of the RNA product from different promoters and use this to calculate the transcription rate of the promoters. Using standard references in our measurements will even allow us to calculate transcription rates in units of Polymerases Per Second (PoPS), which is regarded as the optimal promoter activity measure.
Thank You to Our Sponsors
Technical University of Denmark
Department of Systems Biology
Søltofts Plads 221
2800 Kgs. Lyngby
Denmark
+45 45 25 25 25
dtuigem14@gmail.com .
+45 45 25 25 25
dtuigem14@gmail.com . 
