Team:UESTC-China/Protocol

From 2014.igem.org

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         </div>
         </div>
       </div>
       </div>
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       <div id="SectionTitles2" style="width:500px;">DNA ligation with T4 DNA Ligase
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       <div id="SectionTitles2" style="width:500px;">DNA ligation with Gibson Assemble
         </h1>
         </h1>
         <table border="1" id="BiosensorsTable" cellspacing="0";>
         <table border="1" id="BiosensorsTable" cellspacing="0";>
           <tr>
           <tr>
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             <th class="top">Name</th>
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             <th class="top">Component</th>
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             <th class="top">Type</th>
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             <th class="top">20ul Reaction </th>
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            <th class="top">Description</th>
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            <th class="top">Designer</th>
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            <th class="top">Length</th>
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           </tr>
           </tr>
           <tr>
           <tr>
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             <td class="mid"><a href="https://2013.igem.org/Team:Peking/Project/BioSensors/XylS">XylS</a></td>
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             <td class="mid">Vector DNA</td>
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             <td class="mid"> BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO</td>
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             <td class="mid">50ng</td>
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            <td class="mid"><I>Pseudomonas putida</I></td>
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            <td class="mid">xxxxxxxx</td>
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            <td class="mid">xxxxxxxxxxx</td>
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           </tr>
           </tr>
           <tr>
           <tr>
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             <td class="mid"><a href="https://2013.igem.org/Team:Peking/Project/BioSensors/XylS">XylS</a></td>
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             <td class="mid">Insert DNA</td>
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             <td class="mid"> BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO</td>
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             <td class="mid"> the moles of insert DNA to vector DNA is 5：1</td>
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            <td class="mid"><I>Pseudomonas putida</I></td>
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            <td class="mid">xxxxxxxx</td>
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            <td class="mid">xxxxxxxxxxx</td>
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           </tr>
           </tr>
           <tr>
           <tr>
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             <td class="mid"><a href="https://2013.igem.org/Team:Peking/Project/BioSensors/XylS">XylS</a></td>
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             <td class="mid">10X T4 DNA ligase buffer</td>
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             <td class="mid"> BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO</td>
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             <td class="mid">2ul</td>
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            <td class="mid"><I>Pseudomonas putida</I></td>
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            <td class="mid">xxxxxxxx</td>
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            <td class="mid">xxxxxxxxxxx</td>
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           </tr>
           </tr>
           <tr>
           <tr>
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             <td class="mid"><a href="https://2013.igem.org/Team:Peking/Project/BioSensors/XylS">XylS</a></td>
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             <td class="mid">T4 DNA ligase</td>
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             <td class="mid"> BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO</td>
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             <td class="mid">1ul</td>
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            <td class="mid"><I>Pseudomonas putida</I></td>
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            <td class="mid">xxxxxxxx</td>
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            <td class="mid">xxxxxxxxxxx</td>
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           </tr>
           </tr>
           <tr>
           <tr>
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             <td class="mid"><a href="https://2013.igem.org/Team:Peking/Project/BioSensors/XylS">XylS</a></td>
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             <td class="mid">ddH2O</td>
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             <td class="mid"> BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO</td>
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             <td class="mid">to 20ul</td>
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            <td class="mid"><I>Pseudomonas putida</I></td>
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            <td class="mid">xxxxxxxx</td>
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            <td class="mid">xxxxxxxxxxx</td>
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           </tr>
           </tr>
           <tr>
           <tr>
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             <td class="mid"><a href="https://2013.igem.org/Team:Peking/Project/BioSensors/XylS">XylS</a></td>
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             <td class="mid">Incubate at 25℃ for 12 hours</td>
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             <td class="mid"> BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO</td>
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             <td class="mid"> </td>
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            <td class="mid"><I>Pseudomonas putida</I></td>
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            <td class="mid">xxxxxxxx</td>
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            <td class="mid">xxxxxxxxxxx</td>
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          </tr>
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-
          <tr>
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            <td class="mid"><a href="https://2013.igem.org/Team:Peking/Project/BioSensors/XylS">XylS</a></td>
+
-
            <td class="mid"> BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO</td>
+
-
            <td class="mid"><I>Pseudomonas putida</I></td>
+
-
            <td class="mid">xxxxxxxx</td>
+
-
            <td class="mid">xxxxxxxxxxx</td>
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-
          </tr>
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-
          <tr>
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-
            <td class="mid"><a href="https://2013.igem.org/Team:Peking/Project/BioSensors/XylS">XylS</a></td>
+
-
            <td class="mid"> BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO</td>
+
-
            <td class="mid"><I>Pseudomonas putida</I></td>
+
-
            <td class="mid">xxxxxxxx</td>
+
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            <td class="mid">xxxxxxxxxxx</td>
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-
          </tr>
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-
          <tr>
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            <td class="mid"><a href="https://2013.igem.org/Team:Peking/Project/BioSensors/XylS">XylS</a></td>
+
-
            <td class="mid"> BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO</td>
+
-
            <td class="mid"><I>Pseudomonas putida</I></td>
+
-
            <td class="mid">xxxxxxxx</td>
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-
            <td class="mid">xxxxxxxxxxx</td>
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-
          </tr>
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-
          <tr>
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            <td class="mid"><a href="https://2013.igem.org/Team:Peking/Project/BioSensors/XylS">XylS</a></td>
+
-
            <td class="mid"> BzO; 2-MeBzO; 3-MeBzO; 2,3-DMeBzO; 3,4-DMeBzO</td>
+
-
            <td class="mid"><I>Pseudomonas putida</I></td>
+
-
            <td class="mid">xxxxxxxx</td>
+
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            <td class="mid">xxxxxxxxxxx</td>
+
           </tr>
           </tr>
 +
         
         </table>
         </table>
</div>
</div>

Revision as of 04:21, 24 September 2014

UESTC-China

DNA ligation with T4 DNA Ligase
Component 20ul Reaction
1.33X Master Mix 15ul
Insert DNA the moles of insert DNA to vector DNA is 5：1
>Vector DNA 50ng
ddH2O To 20ul
Incubate at 50℃ for 1 hour
DNA ligation with Gibson Assemble
Component 20ul Reaction
Vector DNA 50ng
Insert DNA the moles of insert DNA to vector DNA is 5：1
10X T4 DNA ligase buffer 2ul
T4 DNA ligase 1ul
ddH2O to 20ul
Incubate at 25℃ for 12 hours

Retrieved from "http://2014.igem.org/Team:UESTC-China/Protocol"