From 2014.igem.org

(Difference between revisions)

Revision as of 16:59, 23 September 2014

iGEM 2014 Measurement Interlab Study Worksheet

Inter-Lab Study Worksheet:

For each assay that you perform, fill out the following worksheet. Answer each question with enough detail to allow another person to replicate your measurements without needing to ask you any questions. This does not necessarily mean you need to describe everything in detail---for example, if you use a standard assay, you just need to give enough information to allow another person to use that assay in the same way that you did.

Section I: Provenance & Release

Who did the actual work to acquire these measurements?

Our laboratory group carried out the experiments this group consists of Anne Sofie Lærke Hansen, Anne Pihl Bali, Caroline Mosbech, Kristian Barreth Karlsen, Kristian Jensen & Rasmus Bech

What other people should be credited for these measurements?

We did all the experimental work for the interlab study ourselves. We did

Ali Altintas assisted us in setting up the BioLector. Anne Egholm helped us to use the Flow Cytometer.

On what dates were the protocols run and the measurements taken?

The applied BioLector experiment ran: 10th-11th of September 2014

The applied Flow cytometry experiment was done on the 22nd of September 2014.

Do all persons involved consent to the inclusion of this data in publications derived from the iGEM interlab study?

Yes.

Section II: Protocols

What protocol did you use to prepare samples for measurement?

Construct strains:

Create multiple strains expressing GFP from different promoters from the Andersen Library. Constructs were prepared with Standard Assembly. (see protocol).

Plasmid were purified with Zyppy™ Plasmid Miniprep Kit from Zymo research following manufacturer's protocol.

Competent DH5α cells were prepared (see protocol).

Purified plasmids were transformed into competent DH5α cells (see protocol).

Culture measurements:

We used cell culture tubes (10 mL) containing 2 mL LB medium (with 0.5% NaCl) and 25 μg/mL chloramphenicol for each strain. To each of the tubes, we inoculated cells from a single colony with a sterile inoculation loop and left it overnight at 37°C, shaking at 350 rpm.

The wells of the sterile BioLector plate (48 well FlowerPlate) was prepared with 1450 μL (1500μL for controls) of LB (0.5% NaCl) containing 25 μg/mL chloramphenicol. The following day we transferred 10 μL of each culture into an Eppendorf tube with 990 μL of sterile physiological salt water (creating a 100-fold dilution). We inoculated the individual BioLector wells with 10 μL of the dilution (creating an additional 150-fold dilution). Each strain was grown in triplicates to make sure we had 3 independent measurements and a more precise result. We incubated the plate in the BioLector at 37°C and 1000 rpm for 12.5 hours (stationary phase was reached). During the growth experiment we measured biomass and GFP fluorescence every 5 minutes. Biomass was measured by light scatter at 620 nm, while fluorescence was measured at 520 nm, with an excitation wavelength of 488 nm.

Single cell measurements:

2 mL LB (0.5% NaCl) was inoculated with cells from a plate and grown overnight.

The cultures were washed and resuspended in PBS.

The cell suspensions were diluted in PBS to a concentration where the flow cytometer could detect 1000-1500 events per second.

What sort of instrument did you use to acquire measurements?

What is the model and manufacturer?

Instrument: BioLector^® (Basic) - Microbioreactor System; Manufacturer: m2p-laps.

Instrument: FACSCalibur Cell Analyzer; Manufacturer: BD Biosciences

How is it configured for your measurements?

The BioLector is fitted with filters for measuring biomass (scatter at 620 nm) and GFP (emission at 520 nm, excitation with 488 nm). Settings: Gain: GFP: 80; Biomass: 30; Humidity: controlled at 95%; Shake: 1000 rpm.

The FACSCalibur was used with settings…Jensen thinks about it…

What protocol did you use to take measurements?

The BioLector was instructed to take measurements every 4.86 minutes. Settings: GFP: 80; Biomass: 30; Humidity: controlled at 95%. 2.5 mL LB medie with proper antibiotics in each well.

The FACS was set up with a threshold on side scatter, as events with low side-scatter are very likely noise, i.e. not actual cells. Each sample was then analysed at 1000-1500 events per second, until a total of 100,000 events was reached.

What method is used to determine whether to include or exclude each sample from the data set?

Strains that showed correct digest patterns were included. Strains that were sequenced and showed wrong sequences were excluded. As were cultures that did not grow, and single replicates that showed negligible fluorescence.

What exactly were the controls that you used?

DH5α with BBa_E0240 (no promoter) was used to determine cell background fluorescence (without GFP). For the BioLector, clean media (without cells) was used to determine media fluorescence.

What quantities were measured?

On the BioLector: GFP fluorescence (488 nm /520 nm) and biomass (scatter at 620nm). The flow cytometer measured forward scatter (cell size), side scatter (cell granularity) and fluorescence.

How much time did it take to acquire each set of measurements?

The running time of the BioLector experiment was 12 hours and 30 minutes. One cycle took 4.86 min.

Flow cytometry: The measurements themselves took less than 1 hour.

How much does it cost to acquire a set of measurements?

Assuming the lab already have a BioLector. For a full plate with samples: Flowerplate for the BioLector, 170mL LB medium, chloramphenicol, 16 pts. inoculation tubes, tips, Eppendorf tubes approximately $150.

What are the practical limits on the number or rate of measurements taken with this instrument and protocol?

BioLector: With the used protocol 48 samples can be measured within short intervals of time, however, 3 of these should be used as control. So when measuring triplicates it is possible to measure 15 different strains in the protocol used for the interlab study. Analysing such a plate takes approximately 12 hours.

Section III: Measured Quantities

For each type of quantity measured, report on the following:

What are the units of the measurement?

All measurements are in arbitrary units in both the BioLector and the FACSCalibur.

What is the equivalent unit expressed as a combination of the seven SI base units?

Not applicable due to arbitrary units.

Precision:

What is the range of possible measured values for this quantity, using your instrument as configured for these measurements?

Linear range for OD 600 is between 0.2 and 600.

Linear range for GFP XXXXX

What are the significant figures for these measurements?

We have estimated a mixed model with promoter as fixed effect and replicates as random effects, this model showed the residual standard deviation as 0.040. With this result we can say that a single measurement is no more precise than the measured value ± 0.040.

Is the precision the same across the entire range? If not, how does it differ?

We have no data to determine if the precision differ through the entire range.

How did you determine these answers?

Jensen thinks about something

Accuracy:

When was the instrument last calibrated?

The machine “self-calibrate” before every cycle, meaning the BioLector sensor/probe returns to the original position and measure a fixed known light intensity. It is approximately 5 years old but the filters have been changed 3 years ago.

How was the instrument calibrated?

The machine “self-calibrate” before every cycle, meaning the BioLector sensor/probe returns to the original position and measure a fixed known light intensity.

Section IV: Measurements

For each sample, report:

The identity of the sample:

Each quantity directly measured:

Each quantity derived from measurements:

For each group of replicates, report:

The identity of samples in the set

Which, if any, of the samples are excluded and why

In two of the triplicate sets, one outlier was excluded: For the promoter J23100, one replicate did not grow. For promoter J23102 one replicate had negligible fluorescence.

The mean and standard deviation for each quantity measured or derived

@@ Line 4: / Line 4: @@
 <div class="pagescontent">
 <maincontent>
 <div class=WordSection1>
-<p class=MsoNormal style='margin-top:10.0pt;margin-right:0cm;margin-bottom:
+<div style='mso-element:para-border-div;border:none;border-bottom:solid windowtext 1.0pt;
-.0pt;margin-left:0cm;line-height:0%;mso-outline-level:1'><span class=SpellE><span
+mso-border-bottom-alt:solid windowtext .5pt;padding:0cm 0cm 1.0pt 0cm'>
-class=GramE><span lang=EN-US style='font-size:20.5pt;font-family:"Georgia","serif";
-mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman";
-color:black;mso-font-kerning:18.0pt;mso-ansi-language:EN-US;mso-fareast-language:
-DA'>iGEM</span></span></span><span lang=EN-US style='font-size:20.5pt;
-font-family:"Georgia","serif";mso-fareast-font-family:"Times New Roman";
-mso-bidi-font-family:"Times New Roman";color:black;mso-font-kerning:18.0pt;
-mso-ansi-language:EN-US;mso-fareast-language:DA'> 2014 Measurement <span
-class=SpellE>Interlab</span> Study Worksheet</span><b><span lang=EN-US
-style='font-size:24.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:
-"Times New Roman";mso-font-kerning:18.0pt;mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></b></p>
-<p class=MsoNormal style='margin-top:4.0pt;margin-right:0cm;margin-bottom:6.0pt;
+<h1 style='margin-bottom:14.0pt;line-height:71%;border:none;mso-border-bottom-alt:
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+solid windowtext .5pt;padding:0cm;mso-padding-alt:0cm 0cm 1.0pt 0cm'><a
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+name=h.6uh8lhm4bpfv></a><span class=SpellE><span class=GramE><span lang=EN-US
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>To participate
+style='font-size:20.0pt;mso-bidi-font-size:10.0pt;line-height:71%;font-family:
-in this study, please complete the following worksheet and submit to
+"Georgia","serif";mso-fareast-font-family:Georgia;mso-bidi-font-family:Georgia;
-measurement [AT] <span class=SpellE>igem</span> [DOT] org.<o:p></o:p></span></p>
+mso-ansi-language:EN-US'>iGEM</span></span></span><span lang=EN-US
+style='font-size:20.0pt;mso-bidi-font-size:10.0pt;line-height:71%;font-family:
+"Georgia","serif";mso-fareast-font-family:Georgia;mso-bidi-font-family:Georgia;
+mso-ansi-language:EN-US'> 2014 Measurement <span class=SpellE>Interlab</span>
+Study Worksheet</span><span lang=EN-US style='mso-ansi-language:EN-US'><o:p></o:p></span></h1>
-<p class=MsoNormal style='margin-top:4.0pt;margin-right:0cm;margin-bottom:6.0pt;
+</div>
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:12.0pt;
-font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";
-mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p>&nbsp;</o:p></span></p>
-<p class=MsoNormal style='margin-top:10.0pt;line-height:0%;mso-outline-level:
+<p class=MsoNormal style='text-align:justify'><a name=h.114kjgc4tbgc></a><b
-'><span lang=EN-US style='font-size:16.0pt;font-family:"Georgia","serif";
+style='mso-bidi-font-weight:normal'><span lang=EN-US style='color:black;
-mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman";
+mso-themecolor:text1;mso-ansi-language:EN-US'>Inter-Lab Study Worksheet:<o:p></o:p></span></b></p>
-color:black;mso-ansi-language:EN-US;mso-fareast-language:DA'>Inter-Lab Study
-Worksheet:</span><b><span lang=EN-US style='font-size:18.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></b></p>
 <p class=MsoNormal style='margin-top:4.0pt;margin-right:0cm;margin-bottom:6.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>For each assay
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>For each assay
 that you perform, fill out the following worksheet. Answer each question with
 enough detail to allow another person to replicate your measurements without
@@ Line 49: / Line 35: @@
 describe everything in detail---for example, if you use a standard assay, you
 just need to give enough information to allow another person to use that assay
-in the same way that you did.</span><span lang=EN-US style='font-size:12.0pt;
+in the same way that you did.<o:p></o:p></span></p>
-font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";
-mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+<p class=MsoNormal style='margin-top:4.0pt;margin-right:0cm;margin-bottom:6.0pt;
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-DA'><o:p>&nbsp;</o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+<p class=MsoNormal style='text-align:justify'><span lang=EN-US
-normal'><span class=SpellE><span style='font-size:11.5pt;font-family:"Arial","sans-serif";
+style='font-size:20.0pt;mso-bidi-font-size:10.0pt;line-height:115%;font-family:
-mso-fareast-font-family:"Times New Roman";color:#414141;mso-fareast-language:
+"Georgia","serif";mso-fareast-font-family:Georgia;mso-bidi-font-family:Georgia;
-DA'>Section</span></span><span style='font-size:11.5pt;font-family:"Arial","sans-serif";
+mso-ansi-language:EN-US'>Section I: Provenance &amp; Release<o:p></o:p></span></p>
-mso-fareast-font-family:"Times New Roman";color:#414141;mso-fareast-language:
-DA'> I: <span class=SpellE>Provenance</span> &amp; Release</span><span
-style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:
-"Times New Roman";mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:71.25pt;text-indent:-18.0pt;line-height:normal;mso-list:l3 level1 lfo1;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-tab-stops:list 36.0pt;vertical-align:baseline'><![if !supportLists]><span
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
+EN-US'>Who did the actual work to acquire these measurements?<o:p></o:p></span></i></b></p>
-style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>Who did the
-actual work to acquire these measurements?<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Our laboratory
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>Our laboratory
 group carried out the experiments this group consists of Anne Sofie Lærke
 Hansen, Anne Pihl Bali, Caroline <span class=SpellE>Mosbech</span>, Kristian <span
 class=SpellE>Barreth</span> Karlsen, <span class=GramE>Kristian</span> Jensen
-&amp; Rasmus Bech</span><span lang=EN-US style='font-size:12.0pt;font-family:
+&amp; Rasmus Bech<o:p></o:p></span></p>
-"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-ansi-language:
-EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;text-indent:0cm;line-height:normal;mso-list:l1 level1 lfo2;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-vertical-align:baseline'><![if !supportLists]><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-style='mso-list:Ignore'>2.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>What other
+EN-US'>What other people should be credited for these measurements?<o:p></o:p></span></i></b></p>
-people should be credited for these measurements? (i.e., who would be an author
-on any resulting <span class=GramE>publication.</span> For example, your
-faculty advisor may have helped design the protocols that you ran.)<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>We did all the
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>We did all the
+experimental work for the <span class=SpellE>interlab</span> study ourselves.
-experiments for the <span class=SpellE>interlab</span> study ourselves…</span><span
+We did <o:p></o:p></span></p>
-lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Ali <span
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>Ali <span
 class=SpellE>Altintas</span> assisted us in setting up the <span class=SpellE>BioLector</span>.
-Anne <span class=SpellE>Egholm</span> helped us to use the Flow Cytometer.</span><span
+Anne <span class=SpellE>Egholm</span> helped us to use the Flow Cytometer.<o:p></o:p></span></p>
-lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:71.25pt;text-indent:0cm;line-height:normal;mso-list:l2 level1 lfo3;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-vertical-align:baseline'><![if !supportLists]><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
-style='mso-list:Ignore'>3.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>On what dates
-were the protocols run and the measurements taken? (<span class=GramE>this</span>
-will often be a range of dates; make sure you say which data was taken at what
-times.)<o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:0cm;line-height:normal'><span class=SpellE><span lang=EN-US
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
-"Times New Roman";color:#980000;mso-ansi-language:EN-US;mso-fareast-language:
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
-DA'>BioLector</span></span><span lang=EN-US style='font-size:11.5pt;font-family:
+EN-US'>On what dates were the protocols run and the measurements taken? <o:p></o:p></span></i></b></p>
-"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";color:#980000;
-mso-ansi-language:EN-US;mso-fareast-language:DA'> was running: 10th-11th of
-September 2014</span><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The applied <span
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>Flow cytometry
+class=SpellE>BioLector</span> experiment ran: 10th-11th of September 2014<o:p></o:p></span></p>
-was done on the 22nd of September. </span><span lang=EN-US style='font-size:
-.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";
-mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;text-indent:0cm;line-height:normal;mso-list:l7 level1 lfo4;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-vertical-align:baseline'><![if !supportLists]><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The applied Flow
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+cytometry <span class=GramE>experiment <span
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
+style='mso-spacerun:yes'> </span>was</span> done on the 22nd of September 2014.
-style='mso-list:Ignore'>4.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<o:p></o:p></span></p>
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>Do all persons
-involved consent to the inclusion of this data in publications derived from the
-<span class=SpellE>iGEM</span> <span class=SpellE>interlab</span> study?<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span style='font-size:11.5pt;font-family:
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";color:#980000;
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-mso-fareast-language:DA'>Yes.</span><span style='font-size:12.0pt;font-family:
-"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:10.0pt;line-height:0%;mso-outline-level:
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-'><span class=SpellE><span style='font-size:16.0pt;font-family:"Georgia","serif";
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman";
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
-color:black;mso-fareast-language:DA'>Section</span></span><span
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
-style='font-size:16.0pt;font-family:"Georgia","serif";mso-fareast-font-family:
+EN-US'>Do all persons involved consent to the inclusion of this data in
-"Times New Roman";mso-bidi-font-family:"Times New Roman";color:black;
+publications derived from the <span class=SpellE>iGEM</span> <span
-mso-fareast-language:DA'> II: Protocol</span><b><span style='font-size:18.0pt;
+class=SpellE>interlab</span> study?<o:p></o:p></span></i></b></p>
-font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";
-mso-fareast-language:DA'><o:p></o:p></span></b></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:71.25pt;text-indent:-18.0pt;line-height:normal;mso-list:l18 level1 lfo5;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-tab-stops:list 36.0pt;vertical-align:baseline'><![if !supportLists]><span
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Yes.<o:p></o:p></span></p>
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
+<span lang=EN-US style='font-size:11.0pt;mso-bidi-font-size:10.0pt;line-height:
-style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
+%;font-family:"Arial","sans-serif";mso-fareast-font-family:Arial;color:black;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
+mso-themecolor:text1;mso-ansi-language:EN-US;mso-fareast-language:DA;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+mso-bidi-language:AR-SA'><br clear=all style='mso-special-character:line-break;
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>What protocol
+page-break-before:always'>
-did you use to prepare samples for measurement?<o:p></o:p></span></p>
+</span>
+<p class=MsoNormal style='text-align:justify'><a name=h.p37mhqcp9erc></a><b
+style='mso-bidi-font-weight:normal'><span lang=EN-US style='color:black;
+mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></b></p>
+<p class=MsoNormal style='text-align:justify'><span lang=EN-US
+style='font-size:20.0pt;mso-bidi-font-size:10.0pt;line-height:115%;font-family:
+"Georgia","serif";mso-fareast-font-family:Georgia;mso-bidi-font-family:Georgia;
+mso-ansi-language:EN-US'>Section II: Protocols<o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
-DA'><o:p>&nbsp;</o:p></span></p>
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
+EN-US'>What protocol did you use to prepare samples for measurement?<o:p></o:p></span></i></b></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><u><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><u><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Construct
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>Construct
+strains: </span></u><span lang=EN-US style='color:black;mso-themecolor:text1;
-strains: </span></u><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+mso-ansi-language:EN-US'><o:p></o:p></span></p>
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Create
-color:#93C47D;mso-ansi-language:EN-US;mso-fareast-language:DA'>Create multiple
+multiple strains expressing GFP from different promoters from the Andersen
-strains expressing GFP from different promoters from the Andersen Library.
+Library. Constructs were prepared with Standard Assembly. (<span class=GramE>see</span>
-Constructs were prepared with Standard Assembly. (<span class=GramE>see</span>
+<a
-protocol). </span><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+href="https://2014.igem.org/Team:DTU-Denmark/Methods/Protocols#standard-assembly-protocol"><span
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
+style='color:black;mso-themecolor:text1'>protocol</span></a>). <o:p></o:p></span></p>
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+<p class=MsoNormal style='text-align:justify'><span class=GramE><span
-normal'><span class=GramE><span lang=EN-US style='font-size:11.5pt;font-family:
+lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Plasmid
-"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";color:#93C47D;
+were</span></span><span lang=EN-US style='color:black;mso-themecolor:text1;
-mso-ansi-language:EN-US;mso-fareast-language:DA'>Plasmid were</span></span><span
+mso-ansi-language:EN-US'> purified with <span class=SpellE><span
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+style='background:white;mso-highlight:white'>Zyppy</span></span><span
-"Times New Roman";color:#93C47D;mso-ansi-language:EN-US;mso-fareast-language:
+style='background:white;mso-highlight:white'>™ Plasmid <span class=SpellE>Miniprep</span>
-DA'> purified with <span class=SpellE><span style='background:white'>Zyppy</span></span><span
+Kit from <span class=SpellE>Zymo</span> research following manufacturer's
-style='background:white'>™ Plasmid <span class=SpellE>Miniprep</span> Kit from <span
+protocol. </span><o:p></o:p></span></p>
-class=SpellE>Zymo</span> research. <span class=GramE>Followed manufacturer's
-protocol.</span> </span></span><span lang=EN-US style='font-size:12.0pt;
-font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";
-mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+<p class=MsoNormal style='text-align:justify'><span lang=EN-US
-normal'><span lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";
+style='color:black;mso-themecolor:text1;background:white;mso-highlight:white;
-mso-fareast-font-family:"Times New Roman";color:#93C47D;background:white;
+mso-ansi-language:EN-US'>Competent DH5&amp;alpha; cells were prepared (see <a
-mso-ansi-language:EN-US;mso-fareast-language:DA'>Competent DH5&amp;alpha; cells
+href="https://2014.igem.org/Team:DTU-Denmark/Methods/Protocols#competent-cell-protocol"><span
-were prepared (see protocol).</span><span lang=EN-US style='font-size:12.0pt;
+style='color:black;mso-themecolor:text1'>protocol</span></a>).</span><span
-font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";
+lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p></o:p></span></p>
-mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+<p class=MsoNormal style='text-align:justify'><span lang=EN-US
-normal'><span lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";
+style='color:black;mso-themecolor:text1;background:white;mso-highlight:white;
-mso-fareast-font-family:"Times New Roman";color:#93C47D;background:white;
+mso-ansi-language:EN-US'>Purified plasmids were transformed into competent
-mso-ansi-language:EN-US;mso-fareast-language:DA'>Purified plasmids were
+DH5&amp;alpha; cells (see <a
-transformed into competent DH5&amp;alpha; cells. </span><span lang=EN-US
+href="https://2014.igem.org/Team:DTU-Denmark/Methods/Protocols#chemical-transformation-protocol"><span
-style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:
+style='color:black;mso-themecolor:text1'>protocol</span></a>). </span><span
-"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
+lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+<p class=MsoNormal style='text-align:justify'><span lang=EN-US
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p>&nbsp;</o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><u><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><u><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Culture
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>Culture
+measurements:</span></u><span lang=EN-US style='color:black;mso-themecolor:
-measurements:</span></u><span lang=EN-US style='font-size:12.0pt;font-family:
+text1;mso-ansi-language:EN-US'><o:p></o:p></span></p>
-"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-ansi-language:
-EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>We used cell
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>We used cell
 culture tubes (10 mL) containing 2 mL LB medium (with 0.5% <span class=SpellE>NaCl</span>)
 and 25 &amp;<span class=SpellE>mu<span class=GramE>;g</span></span>/mL
 chloramphenicol for each strain. To each of the tubes, we inoculated cells from
 a single colony with a sterile inoculation loop and left it overnight at
-&amp;deg<span class=GramE>;C</span>, shaking at 350 rpm. </span><span
+&amp;deg<span class=GramE>;C</span>, shaking at 350 rpm. <o:p></o:p></span></p>
-lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The wells of
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>The wells of the
+the sterile <span class=SpellE>BioLector</span> plate (48 well <span
-sterile <span class=SpellE>BioLector</span> plate (48 well <span class=SpellE>FlowerPlate</span>)
+class=SpellE>FlowerPlate</span>) was prepared with 1450 &amp;<span
-was prepared with 1450 &amp;<span class=SpellE>mu<span class=GramE>;L</span></span>
+class=SpellE>mu<span class=GramE>;L</span></span> (1500&amp;mu;L for controls)
-(1500&amp;mu;L for controls) of LB (0.5% <span class=SpellE>NaCl</span>)
+of LB (0.5% <span class=SpellE>NaCl</span>) containing 25 &amp;<span
-containing 25 &amp;<span class=SpellE>mu;g</span>/mL chloramphenicol. The
+class=SpellE>mu;g</span>/mL chloramphenicol. The following day we transferred
-following day we transferred 10 &amp;<span class=SpellE>mu<span class=GramE>;L</span></span>
+&amp;<span class=SpellE>mu<span class=GramE>;L</span></span> of each culture
-of each culture into an Eppendorf tube with 990 &amp;<span class=SpellE>mu;L</span>
+into an Eppendorf tube with 990 &amp;<span class=SpellE>mu;L</span> of sterile
-of sterile physiological salt water (creating a 100-fold dilution). We
+physiological salt water (creating a 100-fold dilution). We inoculated the
-inoculated the individual <span class=SpellE>BioLector</span> wells with 10
+individual <span class=SpellE>BioLector</span> wells with 10 &amp;<span
-&amp;<span class=SpellE>mu<span class=GramE>;L</span></span> of the dilution
+class=SpellE>mu<span class=GramE>;L</span></span> of the dilution (creating an
-(creating an additional 150-fold dilution). Each strain was grown in
+additional 150-fold dilution). Each strain was grown in triplicates to make
-triplicates to make sure we had 3 independent measurements and a more precise
+sure we had 3 independent measurements and a more precise result. We incubated
-result. We incubated the plate in the <span class=SpellE>BioLector</span> at
+the plate in the <span class=SpellE>BioLector</span> at 37&amp;deg<span
-&amp;deg<span class=GramE>;C</span> and 1000 rpm for 12.5 hours (stationary
+class=GramE>;C</span> and 1000 rpm for 12.5 hours (stationary phase was
-phase was reached). During the growth experiment we measured biomass and GFP
+reached). During the growth experiment we measured biomass and GFP fluorescence
-fluorescence every 5 minutes. Biomass was measured by light scatter at 620 nm,
+every 5 minutes. Biomass was measured by light scatter at 620 nm, while
-while fluorescence was measured at 520 nm, with an excitation wavelength of 488
+fluorescence was measured at 520 nm, with an excitation wavelength of 488 nm.<o:p></o:p></span></p>
-nm.</span><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-DA'><o:p>&nbsp;</o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><u><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><u><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Single cell
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>Single cell
+measurements:</span></u><span lang=EN-US style='color:black;mso-themecolor:
-measurements:</span></u><span lang=EN-US style='font-size:12.0pt;font-family:
+text1;mso-ansi-language:EN-US'><o:p></o:p></span></p>
-"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-ansi-language:
-EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>2 mL LB (0.5% <span
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>2 mL LB (0.5% <span
 class=SpellE>NaCl</span>) was inoculated with cells from a plate and grown
-overnight.</span><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+overnight.<o:p></o:p></span></p>
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The cultures
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>The cultures
+were washed and <span class=SpellE>resuspended</span> in PBS.<o:p></o:p></span></p>
-were washed and <span class=SpellE>resuspended</span> in PBS.</span><span
-lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The cell
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>The cell
 suspensions were diluted in PBS to a concentration where the flow cytometer
-could detect 1000-1500 events per second.</span><span lang=EN-US
+could detect 1000-1500 events per second.<o:p></o:p></span></p>
-style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:
-"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p>&nbsp;</o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;text-indent:0cm;line-height:normal;mso-list:l6 level1 lfo6;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-vertical-align:baseline'><![if !supportLists]><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
-style='mso-list:Ignore'>2.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>What sort of
-instrument did you use to acquire measurements?<o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<i style='mso-bidi-font-style:normal'><span lang=EN-US style='font-size:11.0pt;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l6 level2 lfo7;
+mso-bidi-font-size:10.0pt;line-height:115%;font-family:"Arial","sans-serif";
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+mso-fareast-font-family:Arial;color:black;mso-themecolor:text1;mso-ansi-language:
-lang=EN-US style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+EN-US;mso-fareast-language:DA;mso-bidi-language:AR-SA'><br clear=all
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
+style='mso-special-character:line-break;page-break-before:always'>
-mso-ansi-language:EN-US;mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span
+</span></i>
-style='font:7.0pt "Times New Roman"'>&nbsp; </span></span></span><![endif]><span
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
-DA'>What is the model and manufacturer? <o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormal><i style='mso-bidi-font-style:normal'><span lang=EN-US
-margin-left:36.0pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></i></p>
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#CC0000;mso-ansi-language:EN-US;mso-fareast-language:DA'>Instrument: <span
-class=SpellE>BioLector</span></span><span class=GramE><b><sup><span lang=EN-US
-style='font-size:7.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#CC0000;background:#F6F6F6;mso-ansi-language:EN-US;
-mso-fareast-language:DA'>®</span></sup></b><span lang=EN-US style='font-size:
-.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#CC0000;mso-ansi-language:EN-US;mso-fareast-language:DA'> &nbsp;(</span></span><span
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#CC0000;mso-ansi-language:EN-US;mso-fareast-language:
-DA'>Basic) - <span class=SpellE>Microbioreactor</span> System </span><span
-lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:36.0pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
-color:#CC0000;mso-ansi-language:EN-US;mso-fareast-language:DA'>Manufacturer:
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
-m2p-laps</span><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+EN-US'>What sort of instrument did you use to acquire measurements?<o:p></o:p></span></i></b></p>
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
+text-indent:36.0pt;line-height:150%'><i style='mso-bidi-font-style:normal'><span
-DA'><o:p>&nbsp;</o:p></span></p>
+lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>What
+is the model and manufacturer? <o:p></o:p></span></i></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:36.0pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Instrument: <span
-color:#CC0000;mso-ansi-language:EN-US;mso-fareast-language:DA'>Instrument: <span
+class=SpellE>BioLector</span></span><span class=GramE><b style='mso-bidi-font-weight:
-class=SpellE>FACSCalibur</span> Cell Analyzer</span><span lang=EN-US
+normal'><sup><span lang=EN-US style='font-size:12.0pt;mso-bidi-font-size:10.0pt;
-style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:
+line-height:150%;color:black;mso-themecolor:text1;background:#F6F6F6;
-"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
+mso-ansi-language:EN-US'>®</span></sup></b><span lang=EN-US style='color:black;
+mso-themecolor:text1;mso-ansi-language:EN-US'><span style='mso-spacerun:yes'>
+</span>(</span></span><span lang=EN-US style='color:black;mso-themecolor:text1;
+mso-ansi-language:EN-US'>Basic) - <span class=SpellE>Microbioreactor</span>
+System; Manufacturer: m2p-laps.<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:36.0pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Instrument: <span
-color:#CC0000;mso-ansi-language:EN-US;mso-fareast-language:DA'>Manufacturer: BD
+class=SpellE>FACSCalibur</span> Cell Analyzer; Manufacturer: BD Biosciences<o:p></o:p></span></p>
-Biosciences</span><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+margin-left:36.0pt;text-align:justify;line-height:150%'><span lang=EN-US
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-DA'><o:p>&nbsp;</o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l10 level2 lfo8;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+text-indent:36.0pt;line-height:150%'><i style='mso-bidi-font-style:normal'><span
-lang=EN-US style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>How
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
+is it configured for your measurements?<o:p></o:p></span></i></p>
-mso-ansi-language:EN-US;mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span
-style='font:7.0pt "Times New Roman"'>&nbsp; </span></span></span><![endif]><span
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
-DA'>How is it configured for your measurements? (e.g., light filters, illumination,
-amplification)<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:36.0pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The <span
-color:#00894D;mso-ansi-language:EN-US;mso-fareast-language:DA'>The <span
 class=SpellE>BioLector</span> is fitted with filters for measuring biomass
-(scatter at 620 nm) and GFP (emission at 520 nm, excitation with 488 nm). </span><span
+(scatter at 620 nm) and GFP (emission at 520 nm, excitation with 488 nm). Settings:
-lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+Gain: GFP: 80; Biomass: 30; Humidity: controlled at 95%; <span class=GramE>Shake</span>:
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
+rpm.<o:p></o:p></span></p>
-DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:36.0pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The <span
-color:#00894D;mso-ansi-language:EN-US;mso-fareast-language:DA'>GAIN: </span><span
+class=SpellE>FACSCalibur</span> was used with settings…Jensen thinks about it…<o:p></o:p></span></p>
-lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:36.0pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:36.0pt;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-color:#00894D;mso-ansi-language:EN-US;mso-fareast-language:DA'>GFP: 80 </span><span
-lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:36.0pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
-color:#00894D;mso-ansi-language:EN-US;mso-fareast-language:DA'>Biomass: 30</span><span
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>What protocol
-lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+did you use to take measurements?<o:p></o:p></span></i></p>
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:36.0pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#00894D;mso-ansi-language:EN-US;mso-fareast-language:DA'>Humidity was
-controlled at 95%.</span><span lang=EN-US style='font-size:12.0pt;font-family:
-"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-ansi-language:
-EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p>&nbsp;</o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:36.0pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#00894D;mso-ansi-language:EN-US;mso-fareast-language:DA'>The <span
-class=SpellE>FACSCalibur</span> was used with settings…</span><span lang=EN-US
-style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:
-"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p>&nbsp;</o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;text-indent:0cm;line-height:normal;mso-list:l5 level1 lfo9;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-vertical-align:baseline'><![if !supportLists]><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The <span
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+class=SpellE>BioLector</span> was instructed to take measurements every 4.86
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
+minutes. Settings: GFP: 80; Biomass: 30; Humidity: controlled at 95%. <span
-style='mso-list:Ignore'>3.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+class=GramE>2.5 mL LB <span class=SpellE>medie</span> with proper antibiotics
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
+in each well.</span><o:p></o:p></span></p>
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>What protocol
-did you use to take measurements?<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>The <span
-class=SpellE>BioLector</span> was instructed to take measurements every 5
-minutes.</span><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p>&nbsp;</o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The FACS was
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>The FACS was set
+set up with a threshold on side scatter, as events with low side-scatter are
-up with a threshold on side scatter, as events with low side-scatter are very
+very likely noise, i.e. not actual cells. Each sample was then <span
-likely noise, i.e. not actual cells. Each sample was then <span class=SpellE>analysed</span>
+class=SpellE>analysed</span> at 1000-1500 events per second, until a total of
-at 1000-1500 events per second, until a total of 100,000 events was reached.</span><span
+,000 events was reached.<o:p></o:p></span></p>
-lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p>&nbsp;</o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:71.25pt;text-indent:0cm;line-height:normal;mso-list:l19 level1 lfo10;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-vertical-align:baseline'><![if !supportLists]><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
-style='mso-list:Ignore'>4.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>What method is used
-to determine whether to include or exclude each sample from the data set?<o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><br
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>What method is
-style='mso-special-character:line-break'>
+used to determine whether to include or exclude each sample from the data set?<o:p></o:p></span></i></p>
-<![if !supportLineBreakNewLine]><br style='mso-special-character:line-break'>
-<![endif]></span><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Strains that
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>Strains that
 showed correct digest patterns were included. Strains that were sequenced and
 showed wrong sequences were excluded. As were cultures that did not grow, and
-single replicates that showed negligible fluorescence. </span><span lang=EN-US
+single replicates that showed negligible fluorescence. <o:p></o:p></span></p>
-style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:
-"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-DA'><o:p>&nbsp;</o:p></span></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:0cm;text-indent:0cm;line-height:normal;mso-list:l9 level1 lfo11;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-vertical-align:baseline'><![if !supportLists]><span lang=EN-US
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>What exactly
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
+were the controls that you used?<o:p></o:p></span></i></p>
-style='mso-list:Ignore'>5.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>What exactly
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
-were the controls that you used?<br>
+mso-ansi-language:EN-US'>DH5&amp;alpha; with BBa_E0240 (no promoter) was used
-</span><span lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";
-mso-fareast-font-family:"Times New Roman";color:#980000;mso-ansi-language:EN-US;
-mso-fareast-language:DA'>DH5&amp;alpha; with BBa_E0240 (no promoter) was used
 to determine cell background fluorescence (without GFP). For the <span
-class=SpellE>BioLector</span>, clean media was used to determine media
+class=SpellE>BioLector</span>, clean media (without cells) was used to
-fluorescence.</span><span lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";
+determine media fluorescence.<o:p></o:p></span></p>
-mso-fareast-font-family:"Times New Roman";color:#414141;mso-ansi-language:EN-US;
-mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:0cm;text-indent:0cm;line-height:normal;mso-list:l9 level1 lfo12;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-vertical-align:baseline'><![if !supportLists]><span lang=EN-US
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
-style='mso-list:Ignore'>6.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>What quantities
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>What
-were measured? (e.g., red fluorescence, green fluorescence, optical density)<br>
+quantities were measured?<o:p></o:p></span></i></p>
-</span><span lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";
-mso-fareast-font-family:"Times New Roman";color:#980000;mso-ansi-language:EN-US;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-mso-fareast-language:DA'>On the <span class=SpellE>BioLector</span>: GFP
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
+mso-ansi-language:EN-US'>On the <span class=SpellE>BioLector</span>: GFP
 fluorescence (488 nm /520 nm) and biomass (scatter at 620nm). The flow
 cytometer measured forward scatter (cell size), side scatter (cell granularity)
-and fluorescence.</span><span lang=EN-US style='font-size:11.5pt;font-family:
+and fluorescence.<o:p></o:p></span></p>
-"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";color:#414141;
-mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:71.25pt;text-indent:0cm;line-height:normal;mso-list:l9 level1 lfo13;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-vertical-align:baseline'><![if !supportLists]><span lang=EN-US
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
-style='mso-list:Ignore'>7.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>How much time
-did it take to acquire each set of measurements?<br>
-</span><span lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";
-mso-fareast-font-family:"Times New Roman";color:#00894D;mso-ansi-language:EN-US;
-mso-fareast-language:DA'>The running time of the <span class=SpellE>BioLector</span>
-experiment was 12 hours and 30 minutes. </span><span lang=EN-US
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:36.0pt;text-indent:36.0pt;line-height:normal'><span lang=EN-US
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
-"Times New Roman";color:#00894D;mso-ansi-language:EN-US;mso-fareast-language:
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
-DA'>One cycle: 4.86 min. </span><span lang=EN-US style='font-size:12.0pt;
+EN-US'>How much time did it take to acquire each set of measurements?<o:p></o:p></span></i></b></p>
-font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";
-mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:36.0pt;text-indent:36.0pt;line-height:normal'><span lang=EN-US
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
-"Times New Roman";color:#980000;mso-ansi-language:EN-US;mso-fareast-language:
+mso-ansi-language:EN-US'>The running time of the <span class=SpellE>BioLector</span>
-DA'>Flow cytometry: The measurements themselves took less than 1 hour.</span><span
+experiment was 12 hours and 30 minutes. One cycle took 4.86 min. <o:p></o:p></span></p>
-lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p>&nbsp;</o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:0cm;text-indent:0cm;line-height:normal;mso-list:l12 level1 lfo14;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-vertical-align:baseline'><![if !supportLists]><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Flow
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+cytometry: The measurements themselves took less than 1 hour.<o:p></o:p></span></p>
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
-style='mso-list:Ignore'>8.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>How much does it
-cost to acquire a set of measurements?<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:69.75pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:36.0pt;text-align:justify;text-indent:36.0pt;line-height:150%'><span
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-color:#CC0000;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
-style='mso-tab-count:1'>                    </span>Assuming the lab already
-have a <span class=SpellE>BioLector</span><span class=GramE>..</span><br>
-<span style='mso-tab-count:1'>                    </span>For a full plate with
-samples: <span class=SpellE>Flowerplate</span> for the <span class=SpellE>BioLector</span>,
-mL LB medium, chloramphenicol, 16 pts. <span class=GramE>inoculation</span>
-tubes, tips, Eppendorf tubes approximately $100.</span><span lang=EN-US
-style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:
-"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:71.25pt;text-indent:0cm;line-height:normal;mso-list:l15 level1 lfo15;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-vertical-align:baseline'><![if !supportLists]><span style='font-size:11.5pt;
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
-font-family:"Arial","sans-serif";mso-fareast-font-family:Arial;color:#414141;
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
-mso-fareast-language:DA'><span style='mso-list:Ignore'>9.<span
+EN-US'>How much does it cost to acquire a set of measurements?<o:p></o:p></span></i></b></p>
-style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>What are the
-practical limits on the number or rate of measurements taken with this instrument
-and protocol?<br>
-<span class=SpellE>BioLector</span>: </span><span lang=EN-US style='font-size:
-.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>With the used
-protocol 48 samples can be measured within short intervals of time, however, 3
-of these should be used as control. So when measuring triplicates it is
-possible to measure 15 different strains in the protocol used for the <span
-class=SpellE>interlab</span> study. </span><span class=SpellE><span
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#980000;mso-fareast-language:DA'>Analysing</span></span><span
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#980000;mso-fareast-language:DA'> <span class=SpellE>such</span>
-a <span class=SpellE>plate</span> <span class=SpellE>takes</span> <span
-class=SpellE>approximately</span> 24 <span class=SpellE>hours</span>.</span><span
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-normal'><span style='font-size:12.0pt;font-family:"Times New Roman","serif";
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-mso-fareast-font-family:"Times New Roman";mso-fareast-language:DA'><o:p>&nbsp;</o:p></span></p>
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
+mso-ansi-language:EN-US'>Assuming the lab already <span class=GramE>have</span>
+a <span class=SpellE>BioLector</span>.<span style='mso-tab-count:1'>  </span>For
+a full plate with samples: <span class=SpellE>Flowerplate</span> for the <span
+class=SpellE>BioLector</span>, 170mL LB medium, chloramphenicol, 16 pts. <span
+class=GramE>inoculation</span> tubes, tips, Eppendorf tubes approximately $150.<i
+style='mso-bidi-font-style:normal'><o:p></o:p></i></span></p>
-<p class=MsoNormal style='margin-top:10.0pt;line-height:0%;mso-outline-level:
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-'><span class=SpellE><span style='font-size:16.0pt;font-family:"Georgia","serif";
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman";
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
-color:black;mso-fareast-language:DA'>Section</span></span><span
+mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-style='font-size:16.0pt;font-family:"Georgia","serif";mso-fareast-font-family:
-"Times New Roman";mso-bidi-font-family:"Times New Roman";color:black;
-mso-fareast-language:DA'> III: <span class=SpellE>Measured</span> <span
-class=SpellE>Quantities</span></span><b><span style='font-size:18.0pt;
-font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";
-mso-fareast-language:DA'><o:p></o:p></span></b></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:71.25pt;text-indent:-18.0pt;line-height:normal;mso-list:l11 level1 lfo16;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-tab-stops:list 36.0pt;vertical-align:baseline'><![if !supportLists]><span
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
+EN-US'>What are the practical limits on the number or rate of measurements
-style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
+taken with this instrument and protocol?<o:p></o:p></span></i></b></p>
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>For each type of
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-quantity measured (e.g., fluorescence, optical density), report on the
+line-height:150%'><span class=SpellE><span lang=EN-US style='color:black;
-following:<o:p></o:p></span></p>
+mso-themecolor:text1;mso-ansi-language:EN-US'>BioLector</span></span><span
+lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>: With
+the used protocol 48 samples can be measured within short intervals of time,
+however, 3 of these should be used as control. So when measuring triplicates it
+is possible to measure 15 different strains in the protocol used for the <span
+class=SpellE>interlab</span> study. <span class=SpellE>Analysing</span> such a
+plate takes approximately 12 hours.<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:71.25pt;text-indent:-18.0pt;line-height:normal;mso-list:l11 level1 lfo16;
+margin-left:0cm;line-height:150%'><span lang=EN-US style='mso-ansi-language:
-tab-stops:list 36.0pt;vertical-align:baseline'><![if !supportLists]><span
+EN-US'><o:p>&nbsp;</o:p></span></p>
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-Arial;color:#414141;mso-fareast-language:DA'><span style='mso-list:Ignore'>2.<span
+<p class=MsoNormal style='text-align:justify'><a name=h.30823x9n23kf></a><span
-style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp; </span></span></span><![endif]><span
+lang=EN-US style='font-size:20.0pt;mso-bidi-font-size:10.0pt;line-height:115%;
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+font-family:"Georgia","serif";mso-fareast-font-family:Georgia;mso-bidi-font-family:
-"Times New Roman";color:#414141;mso-fareast-language:DA'>Units:<o:p></o:p></span></p>
+Georgia;mso-ansi-language:EN-US'>Section III: Measured Quantities<o:p></o:p></span></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
+EN-US'>For each type of quantity measured, report on the following:<o:p></o:p></span></i></b></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>What are the
+units of the measurement?<o:p></o:p></span></i></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l11 level2 lfo17;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>All
-style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+measurements are in arbitrary units in both the <span class=SpellE>BioLector</span>
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
+and the <span class=SpellE>FACSCalibur</span>.<o:p></o:p></span></p>
-mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span style='font:7.0pt "Times New Roman"'>&nbsp;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>What are the
-units of the measurement? </span><span style='font-size:11.5pt;font-family:
-"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";color:#414141;
-mso-fareast-language:DA'>(<span class=SpellE>e.g</span>., meters, <span
-class=SpellE>molecules</span>)<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:108.0pt;text-indent:36.0pt;line-height:normal'><span lang=EN-US
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-"Times New Roman";color:#CC0000;mso-ansi-language:EN-US;mso-fareast-language:
-DA'>All measurements are in arbitrary units.</span><span lang=EN-US
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>What is the
+equivalent unit expressed as a combination of the seven SI base units?<o:p></o:p></span></i></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l13 level2 lfo18;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Not applicable
-style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+due to arbitrary units.<o:p></o:p></span></p>
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
-mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span style='font:7.0pt "Times New Roman"'>&nbsp;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>What is the
-equivalent unit expressed as a combination of the seven SI base units? </span><span
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-fareast-language:DA'>(<a
-href="http://en.wikipedia.org/wiki/SI_base_unit"><span style='color:#414141'>http://en.wikipedia.org/wiki/SI_base_unit</span></a>)<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:108.0pt;text-indent:36.0pt;line-height:normal'><span
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-"Times New Roman";color:#980000;mso-fareast-language:DA'>Not <span
-class=SpellE>applicable</span>.</span><span style='font-size:12.0pt;font-family:
-"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<i style='mso-bidi-font-style:normal'><span lang=EN-US style='font-size:11.0pt;
-margin-left:0cm;text-indent:0cm;line-height:normal;mso-list:l17 level1 lfo19;
+mso-bidi-font-size:10.0pt;line-height:115%;font-family:"Arial","sans-serif";
-vertical-align:baseline'><![if !supportLists]><span style='font-size:11.5pt;
+mso-fareast-font-family:Arial;color:black;mso-themecolor:text1;mso-ansi-language:
-font-family:"Arial","sans-serif";mso-fareast-font-family:Arial;color:#414141;
+EN-US;mso-fareast-language:DA;mso-bidi-language:AR-SA'><br clear=all
-mso-fareast-language:DA'><span style='mso-list:Ignore'>3.<span
+style='mso-special-character:line-break;page-break-before:always'>
-style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
+</span></i>
-</span></span></span><![endif]><span style='font-size:11.5pt;font-family:"Arial","sans-serif";
-mso-fareast-font-family:"Times New Roman";color:#414141;mso-fareast-language:
+<p class=MsoNormal style='text-align:justify'><i style='mso-bidi-font-style:
-DA'>Precision:<o:p></o:p></span></p>
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
+EN-US'><o:p>&nbsp;</o:p></span></i></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
+EN-US'>Precision:<o:p></o:p></span></i></b></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
+margin-bottom:2.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>What is the
+range of possible measured values for this quantity, using your instrument as
+configured for these measurements?<o:p></o:p></span></i></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:2.0pt;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l17 level2 lfo20;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Linear range
-lang=EN-US style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+for OD 600 is between 0.2 and 600.<o:p></o:p></span></p>
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
-mso-ansi-language:EN-US;mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span
-style='font:7.0pt "Times New Roman"'>&nbsp; </span></span></span><![endif]><span
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
-DA'>What is the range of possible measured values for this quantity, using your
-instrument as configured for these measurements? (e.g., a meter stick measures
-in the range of 0 to 1 meter)<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:2.0pt;
-margin-left:36.0pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Linear range
-color:#980000;mso-ansi-language:EN-US;mso-fareast-language:DA'>Linear range for
+for GFP XXXXX<o:p></o:p></span></p>
-OD 600 is between 0.2 and 600.<br>
-</span><span class=SpellE><span style='font-size:11.5pt;font-family:"Arial","sans-serif";
-mso-fareast-font-family:"Times New Roman";color:#980000;mso-fareast-language:
-DA'>Linear</span></span><span style='font-size:11.5pt;font-family:"Arial","sans-serif";
-mso-fareast-font-family:"Times New Roman";color:#980000;mso-fareast-language:
-DA'> range for GFP XXXXX</span><span style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-fareast-language:DA'><o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:2.0pt;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l8 level2 lfo21;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-lang=EN-US style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-mso-ansi-language:EN-US;mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span
+margin-bottom:2.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-style='font:7.0pt "Times New Roman"'>&nbsp; </span></span></span><![endif]><span
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>What are the
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
+significant figures for these measurements?<o:p></o:p></span></i></p>
-DA'>What are the significant figures for <span class=GramE>these measurement</span>?
-(e.g., on a meter stick, it is common to measure to the nearest millimeter).<br>
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-</span><span lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";
+margin-bottom:2.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-mso-fareast-font-family:"Times New Roman";color:#CC4125;mso-ansi-language:EN-US;
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
-mso-fareast-language:DA'>We have estimated a mixed model with promoter as fixed
+mso-ansi-language:EN-US'>We have estimated a mixed model with promoter as fixed
 effect and replicates as random effects, this model showed the residual
 standard deviation as 0.040. With this result we can say that a single
-measurement is no more precise than the measured value ± 0.040.</span><span
+measurement is no more precise than the measured value ± 0.040.<o:p></o:p></span></p>
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
-normal'><span lang=EN-US style='font-size:12.0pt;font-family:"Times New Roman","serif";
-mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:
-DA'><o:p>&nbsp;</o:p></span></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:2.0pt;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l16 level2 lfo22;
+margin-left:36.0pt;text-align:justify;line-height:150%'><span lang=EN-US
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
-mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span style='font:7.0pt "Times New Roman"'>&nbsp;
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>Is the precision
-the same across the entire range? If not, how does it differ?<br>
-</span><span class=SpellE><span style='font-size:11.5pt;font-family:"Arial","sans-serif";
-mso-fareast-font-family:"Times New Roman";color:#414141;mso-fareast-language:
-DA'>Presumeably</span></span><span style='font-size:11.5pt;font-family:"Arial","sans-serif";
-mso-fareast-font-family:"Times New Roman";color:#414141;mso-fareast-language:
-DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:2.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l16 level2 lfo22;
+margin-bottom:2.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
-lang=EN-US style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Is the
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
+precision the same across the entire range? If not, how does it differ?<o:p></o:p></span></i></p>
-mso-ansi-language:EN-US;mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span
-style='font:7.0pt "Times New Roman"'>&nbsp; </span></span></span><![endif]><span
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
-DA'>How did you determine these answers?<o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-margin-left:0cm;text-indent:0cm;line-height:normal;mso-list:l16 level1 lfo23;
+margin-bottom:2.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-vertical-align:baseline'><![if !supportLists]><span style='font-size:11.5pt;
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
-font-family:"Arial","sans-serif";mso-fareast-font-family:Arial;color:#414141;
+mso-ansi-language:EN-US'>We have no data to determine if the precision differ
-mso-fareast-language:DA'><span style='mso-list:Ignore'>2.<span
+through the entire range.<i style='mso-bidi-font-style:normal'><o:p></o:p></i></span></p>
-style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
-</span></span></span><![endif]><span class=SpellE><span style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-fareast-language:DA'>Accuracy</span></span><span
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-fareast-language:DA'>:<o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l16 level2 lfo23;
+margin-bottom:2.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
-lang=EN-US style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></i></p>
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
-mso-ansi-language:EN-US;mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span
-style='font:7.0pt "Times New Roman"'>&nbsp; </span></span></span><![endif]><span
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
-DA'>When <span class=GramE>was the instrument</span> last calibrated?<o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-margin-left:0cm;line-height:normal'><span class=GramE><span lang=EN-US
+margin-bottom:2.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
-"Times New Roman";color:#CC0000;mso-ansi-language:EN-US;mso-fareast-language:
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>How did you
-DA'>approximately</span></span><span lang=EN-US style='font-size:11.5pt;
+determine these answers?<o:p></o:p></span></i></p>
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#CC0000;mso-ansi-language:EN-US;mso-fareast-language:DA'> 5 years old
-machine. &nbsp;Filters changed 3 years ago. </span><span lang=EN-US
-style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:
-"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-margin-left:0cm;line-height:normal'><span class=SpellE><span class=GramE><span
+margin-bottom:2.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
-"Times New Roman";color:#CC0000;mso-fareast-language:DA'>self</span></span></span><span
+mso-ansi-language:EN-US'>Jensen thinks about something<o:p></o:p></span></p>
-class=GramE><span style='font-size:11.5pt;font-family:"Arial","sans-serif";
-mso-fareast-font-family:"Times New Roman";color:#CC0000;mso-fareast-language:
-DA'> <span class=SpellE>calibration</span> <span class=SpellE>every</span> <span
-class=SpellE>cycle</span>.</span></span><span style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#CC0000;mso-fareast-language:DA'> </span><span style='font-size:12.0pt;
-font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";
-mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l0 level2 lfo24;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
-lang=EN-US style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
-mso-ansi-language:EN-US;mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span
-style='font:7.0pt "Times New Roman"'>&nbsp; </span></span></span><![endif]><span
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
-DA'>How was the instrument calibrated?<o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:10.0pt;line-height:0%;mso-outline-level:
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
-'><span class=SpellE><span style='font-size:16.0pt;font-family:"Georgia","serif";
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-mso-fareast-font-family:"Times New Roman";mso-bidi-font-family:"Times New Roman";
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
-color:black;mso-fareast-language:DA'>Section</span></span><span
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
-style='font-size:16.0pt;font-family:"Georgia","serif";mso-fareast-font-family:
+EN-US'>Accuracy:<o:p></o:p></span></i></b></p>
-"Times New Roman";mso-bidi-font-family:"Times New Roman";color:black;
-mso-fareast-language:DA'> IV: Measurements</span><b><span style='font-size:
-.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:"Times New Roman";
-mso-fareast-language:DA'><o:p></o:p></span></b></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-margin-left:71.25pt;text-indent:-18.0pt;line-height:normal;mso-list:l4 level1 lfo25;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-tab-stops:list 36.0pt;vertical-align:baseline'><![if !supportLists]><span
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>When <span
-Arial;color:#414141;mso-fareast-language:DA'><span style='mso-list:Ignore'>1.<span
+class=GramE>was the instrument</span> last calibrated?<o:p></o:p></span></i></p>
-style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp; </span></span></span><![endif]><span
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-fareast-language:DA'>For <span
-class=SpellE>each</span> sample, <span class=SpellE>report</span>:<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l4 level2 lfo26;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The machine “self-calibrate”
-style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+before every cycle, meaning the <span class=SpellE>BioLector</span> sensor/probe
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
+returns to the original position and measure a fixed known light intensity. It
-mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span style='font:7.0pt "Times New Roman"'>&nbsp;
+is approximately 5 years old but the filters have been changed 3 years ago.<o:p></o:p></span></p>
-</span></span></span><![endif]><span style='font-size:11.5pt;font-family:"Arial","sans-serif";
-mso-fareast-font-family:"Times New Roman";color:#414141;mso-fareast-language:
-DA'>the <span class=SpellE>identity</span> of the sample<o:p></o:p></span></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l4 level2 lfo26;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
-mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span style='font:7.0pt "Times New Roman"'>&nbsp;
-</span></span></span><![endif]><span class=SpellE><span style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-fareast-language:DA'>each</span></span><span
-style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-fareast-language:DA'> <span class=SpellE>quantity</span>
-<span class=SpellE>directly</span> <span class=SpellE>measured</span><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l4 level2 lfo26;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
-lang=EN-US style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>How was the
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
+instrument calibrated?<o:p></o:p></span></i></p>
-mso-ansi-language:EN-US;mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span
-style='font:7.0pt "Times New Roman"'>&nbsp; </span></span></span><![endif]><span
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
-DA'>each quantity derived from measurements (e.g., fluorescence/OD)<o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:3.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
-margin-left:71.25pt;text-indent:-18.0pt;line-height:normal;mso-list:l4 level1 lfo26;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
-tab-stops:list 36.0pt;vertical-align:baseline'><![if !supportLists]><span
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+mso-ansi-language:EN-US'>The machine “self-calibrate” before every cycle,
-Arial;color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'><span
+meaning the <span class=SpellE>BioLector</span> sensor/probe returns to the
-style='mso-list:Ignore'>2.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;
+original position and measure a fixed known light intensity.<o:p></o:p></span></p>
-</span></span></span><![endif]><span lang=EN-US style='font-size:11.5pt;
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
-color:#414141;mso-ansi-language:EN-US;mso-fareast-language:DA'>For each group
-of replicates, report:<o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<b style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:16.0pt;
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l4 level2 lfo26;
+mso-bidi-font-size:10.0pt;line-height:115%;font-family:"Georgia","serif";
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+mso-fareast-font-family:Georgia;mso-bidi-font-family:Georgia;color:black;
-lang=EN-US style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+mso-themecolor:text1;mso-ansi-language:EN-US;mso-fareast-language:DA;
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
+mso-bidi-language:AR-SA'><br clear=all style='mso-special-character:line-break;
-mso-ansi-language:EN-US;mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span
+page-break-before:always'>
-style='font:7.0pt "Times New Roman"'>&nbsp; </span></span></span><![endif]><span
+</span></b>
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
-DA'>the identity of samples in the set<o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormal style='text-align:justify'><a name=h.fb7tbh49med6></a><span
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l4 level2 lfo26;
+lang=EN-US style='font-size:16.0pt;mso-bidi-font-size:10.0pt;line-height:115%;
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+font-family:"Georgia","serif";mso-fareast-font-family:Georgia;mso-bidi-font-family:
-lang=EN-US style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+Georgia;color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
-mso-ansi-language:EN-US;mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span
+<p class=MsoNormal style='text-align:justify'><span lang=EN-US
-style='font:7.0pt "Times New Roman"'>&nbsp; </span></span></span><![endif]><span
+style='font-size:20.0pt;mso-bidi-font-size:10.0pt;line-height:115%;font-family:
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
+"Georgia","serif";mso-fareast-font-family:Georgia;mso-bidi-font-family:Georgia;
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
+mso-ansi-language:EN-US'>Section IV: Measurements<o:p></o:p></span></p>
-DA'>which, if any, of the samples are excluded and why<o:p></o:p></span></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
+EN-US'>For each sample, report:<o:p></o:p></span></i></b></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The identity
+of the sample:<o:p></o:p></span></i></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></i></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Each quantity
+directly measured:<o:p></o:p></span></i></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
+mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Each quantity
+derived from measurements:<o:p></o:p></span></i></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><span lang=EN-US style='color:black;mso-themecolor:text1;
+mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:3.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><b style='mso-bidi-font-weight:normal'><i style='mso-bidi-font-style:
+normal'><span lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:
+EN-US'>For each group of replicates, report:<o:p></o:p></span></i></b></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The identity
+of samples in the set<o:p></o:p></span></i></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+text-indent:36.0pt;line-height:150%'><i style='mso-bidi-font-style:normal'><span
+lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></i></p>
+<p class=MsoNormalCxSpMiddle style='margin-top:6.0pt;margin-right:0cm;
+margin-bottom:1.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;
+line-height:150%'><i style='mso-bidi-font-style:normal'><span lang=EN-US
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>Which, if any,
+of the samples are excluded and why<o:p></o:p></span></i></p>
 <p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
-margin-left:36.0pt;line-height:normal'><span lang=EN-US style='font-size:11.5pt;
+margin-left:0cm;text-align:justify;line-height:150%'><span lang=EN-US
-font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
+style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>In two of the
-color:#CC0000;mso-ansi-language:EN-US;mso-fareast-language:DA'>In two of the
 triplicate sets, one outlier was excluded: For the promoter J23100, one
-replicate did not grow. </span><span style='font-size:11.5pt;font-family:"Arial","sans-serif";
+replicate did not grow. For promoter J23102 one replicate had negligible
-mso-fareast-font-family:"Times New Roman";color:#CC0000;mso-fareast-language:
+fluorescence.<o:p></o:p></span></p>
-DA'>For promoter J23102 <span class=SpellE>one</span> <span class=SpellE>replicate</span>
-had <span class=SpellE>negligible</span> <span class=SpellE>fluorescence</span>.</span><span
-style='font-size:12.0pt;font-family:"Times New Roman","serif";mso-fareast-font-family:
-"Times New Roman";mso-fareast-language:DA'><o:p></o:p></span></p>
-<p class=MsoNormal style='margin-top:6.0pt;margin-right:0cm;margin-bottom:1.0pt;
+<p class=MsoNormalCxSpLast style='margin-top:6.0pt;margin-right:0cm;margin-bottom:
-margin-left:123.75pt;text-indent:-18.0pt;line-height:normal;mso-list:l14 level2 lfo27;
+.0pt;margin-left:0cm;mso-add-space:auto;text-align:justify;line-height:150%'><span
-tab-stops:list 72.0pt;vertical-align:baseline'><![if !supportLists]><span
+lang=EN-US style='color:black;mso-themecolor:text1;mso-ansi-language:EN-US'>The
-lang=EN-US style='font-size:10.0pt;mso-bidi-font-size:11.5pt;font-family:Wingdings;
+mean and standard deviation for each quantity measured or derived<o:p></o:p></span></p>
-mso-fareast-font-family:Wingdings;mso-bidi-font-family:Wingdings;color:#414141;
-mso-ansi-language:EN-US;mso-fareast-language:DA'><span style='mso-list:Ignore'>§<span
-style='font:7.0pt "Times New Roman"'>&nbsp; </span></span></span><![endif]><span
-lang=EN-US style='font-size:11.5pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
-"Times New Roman";color:#414141;mso-ansi-language:EN-US;mso-fareast-language:
-DA'>the mean and standard deviation for each quantity measured or derived<o:p></o:p></span></p>
-<p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
+</div>
-</div>
 </maincontent>
 </div>
 </body>
 </html>

Team:DTU-Denmark/Interlab form

From 2014.igem.org

Revision as of 16:59, 23 September 2014

iGEM 2014 Measurement Interlab Study Worksheet