Team:Kyoto/Notebook/DMS Synthesis

From 2014.igem.org

(Difference between revisions)
(Replaced content with "{{Kyoto/css}} {{Kyoto/small_countdown}} <html> <style type="text/css"> body { background-color: #ffffff; } </style> <div id="page"> <a href="https://2014.igem.org/wiki/ind...")
Line 13: Line 13:
-
<a name=“半角小文字日付（ex. September23）” class=“kyoto-jump”></a>
 
-
<h3>日付</h3>
 
-
<h4>Liquid Culture</h4>
 
-
<span class="author">名前</span>
 
-
<table class="note">
 
-
<tr><th>Sample</th><th>Medium</th></tr>
 
-
<tr><td>サンプル名</td><td>培地</td></tr>
 
-
</table>
 
-
 
-
 
-
<a name=“半角小文字日付（ex. September23）” class=“kyoto-jump”></a>
 
-
<h3>日付</h3>
 
-
<h4>Gel Extraction</h4>
 
-
<span class="author">名前</span>
 
-
<table class="note">
 
-
<tr><th>Lane</th><th>Sample</th></tr>
 
-
<tr><td>レーン番号</td><td>サンプル名</td></tr>
 
-
</table>
 
-
 
-
 
-
<a name=“半角小文字日付（ex. September23）” class=“kyoto-jump”></a>
 
-
<h3>日付</h3>
 
-
<h4>Electrophoresis</h4>
 
-
<span class="author">名前</span>
 
-
<table class="note">
 
-
<tr><th>Lane</th><th>Sample</th><th>Ensyme1</th><th>Ensyme2</th></tr>
 
-
<tr><td>レーン番号</td><td>サンプル名</td><td>制限酵素1</td><td>制限酵素2</td></tr>
 
-
</table>
 
-
 
-
 
-
<a name=“半角小文字日付（ex. September23）” class=“kyoto-jump”></a>
 
-
<h3>日付</h3>
 
-
<h4>Miniprep/DNA Purification</h4>
 
-
<span class="author">名前</span>
 
-
<table class="note">
 
-
<tr><th>Sample</th><th>Concentration(&micro;g/mL)</th><th>260/280</th><th>260/230</th></tr>
 
-
<tr><td>サンプル名</td><td>(Concentration)</td><td>(260/280)</td><td>(260/280)</td></tr>
 
-
</table>
 
-
 
-
 
-
<a name=“半角小文字日付（ex. September23）” class=“kyoto-jump”></a>
 
-
<h3>日付</h3>
 
-
<h4>Restriction Enzyme Digestion</h4>
 
-
<span class="author">名前</span>
 
-
<table class="note">
 
-
<tr><th colspan=2>Sample</th><th>EcoR1</th><th>Xba1</th><th>Spe1</th><th>Pst1</th><th colspan=2>Buffer</th><th>BSA</th><th>MilliQ</th><th>Total</tr>
 
-
<tr><td>サンプル名</td><td>(サンプル名)</td><td>(E)</td><td>(X)</td><td>(S)</td><td>(P)</td><td>(HorM)<td>(Buffer)</td></td><td>(BSA)</td><td>(MilliQ)</td><td>(Total)</td></tr>
 
-
</table>
 
-
 
-
 
-
<a name=“半角小文字日付（ex. September23）” class=“kyoto-jump”></a>
 
-
<h3>日付</h3>
 
-
<h4>Ligation</h4>
 
-
<span class="author">名前</span>
 
-
<table class="note">
 
-
<tr><th colspan=2>Vector</th><th colspan=2>Insert</th><th colspan=2>Buffer</th><th colspan=2>Ligase</th><th>MilliQ</th><th>Total</tr>
 
-
<tr><td>ベクター名</td><td>(ベクター)</td><td>インサート名</td><td>(インサート)</td><td>(HorM)</td><td>(Buffer)</td><td>Ligase名</td><td>(Ligase)</td><td>(MilliQ)</td><td>(Total)</td></tr>
 
-
</table>
 
-
 
-
 
-
<a name=“半角小文字日付（ex. September23）” class=“kyoto-jump”></a>
 
-
<h3>日付</h3>
 
-
<h4>Transformation</h4>
 
-
<span class="author">名前</span>
 
-
<table class="note">
 
-
<tr><th colspan=2>Sample</th><th colspan=2>CompetentCells</th><th>Total</th><th>Medium</th></tr>
 
-
<tr><td>サンプル名</td><td>(サンプル)</td><td>コンピ名</td><td>(コンピ)</td><td>(Total)</td><td>培地</td></tr>
 
-
</table>
 
-
 
-
 
-
<a name=“半角小文字日付（ex. September23）” class=“kyoto-jump”></a>
 
-
<h3>日付</h3>
 
-
<h4>Dephosphorylation</h4>
 
-
<span class="author">名前</span>
 
-
<table class="note">
 
-
<tr><th colspan=2>Sample</th></tr>
 
-
<tr><td>サンプル名</td><td>(サンプル)</td></tr>
 
-
</table>
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
<h2>7/31</h2>
 
-
<h3>Restriction Enzyme Digestion</h3>
 
-
<span class="author">Nakamura Matsumoto</span>
 
-
<table class="note">
 
-
<tr><th>Sample</th><th>Volume</th><th>EcoR1</th><th>Spe1</th><th>Buffer(H)</th><th>MilliQ</th><th>TOTAL</tr>
 
-
<tr><td>3/24 PCR(dddD)cloning</td><td>3.2</td><td>1</td><td>1</td><td>3</td><td>21.8</td><td>30</td></tr>
 
-
<tr><td>pSB1C3</td><td>18.5</td><td>1</td><td>1</td><td>3</td><td>6.5</td><td>30</td></tr>
 
-
<tr><td>pSB1C3(control)</td><td>6.2</td><td>0</td><td>0</td><td>0</td><td>2.8</td><td>10</td></tr>
 
-
</table>
 
-
 
-
<h2>8/1</h2>
 
-
<h3>Gel Extraction</h3>
 
-
<span class="author">Yasuda Murata</span>
 
-
<table class="note">
 
-
<tr><th>Lane</th><th>DNA</th>
 
-
<tr><td>1</td><td>1kbp ladder</td></tr>
 
-
<tr><td>2</td><td>3/24 PCR(dddD)cloning E,S</td></tr>
 
-
<tr><td>3</td><td>3/24 PCR(dddD)cloning E,S</td></tr>
 
-
<tr><td>4</td><td>Blank</td></tr>
 
-
<tr><td>5</td><td>pSB1C3</td></tr>
 
-
<tr><td>6</td><td>pSB1C3</td></tr>
 
-
<tr><td>7</td><td>Blank</td></tr>
 
-
<tr><td>8</td><td>1kbp ladder</td></tr>
 
-
<tr><td>9</td><td>3/24 PCR(dddD)cloning(control)</td></tr>
 
-
<tr><td>10</td><td>pSB1C3(control)</td></tr>
 
-
</table>
 
-
 
-
<h2>8/1</h2>
 
-
<h3>Ligation</h3>
 
-
<span class="author">Yasuda</span>
 
-
<table class="note">
 
-
<tr><th>Vector</th><th>inserter</th><th>10xt4LigaseBuffer</th><th>t4Ligase</th><th>MilliQ</th><th>TOTAL</tr>
 
-
<tr><td>1.5 &micro;L(8/1 pSB1C3 2047bp 13.3&micro;g/mL 20μg) </td><td>6.9 &micro;L(8/1 dddD 2545bp 10.8&micro;g/mL 74.60&micro;g) </td><td>2&micro;L </td><td>1&micro;L </td><td>8.6&micro;L </td><td>20&micro;L </td></tr>
 
-
</table>
 
-
 
-
<h2>8/1</h2>
 
-
<h3>transformation</h3>
 
-
<span class="author">Murata</span>
 
-
<table class="note">
 
-
<tr><th>Name</th><th>Sample/(&micro;l)</th><th>Competent Cells/(&micro;l)</th><th>Total/(&micro;l)</th><th>Plate</th></tr>
 
-
<tr><td>pSB1C3</td><td>3</td><td>30</td><td>165</td><td>SOC(132&micro;l)</td></tr>
 
-
</table>
 
-
 
-
<h2>8/2</h2>
 
-
<h3>PCR Cloning</h3>
 
-
<span class="author">Murata</span>
 
-
<table class="note">
 
-
<tr><th></th><th>dddD 121&micro;g/&micro;L</th></tr>
 
-
<tr><td>2x KAPA HiFiHotStartReadyMix</td><td>12.5&micro;L</td></tr>
 
-
<tr><td>F Pre-dddD cloning 62</td><td>0.75&micro;L</td></tr>
 
-
<tr><td>Rv Spe1-dddDcloning 68</td><td>0.75&micro;L</td></tr>
 
-
<tr><td>MilliQ</td><td>11&micro;L</td></tr>
 
-
<tr><td>TOTAL</td><td>25&micro;L</td></tr>
 
-
</table>
 
-
 
-
<h2>8/2</h2>
 
-
<h3>Colony PCR</h3>
 
-
<span class="author">Honda Matsumoto Nakamura</span>
 
-
<table class="note">
 
-
<tr><th></th><th>Master Mix</th></tr>
 
-
<tr><td>Quick Taq</td><td>15&micro;L</td></tr>
 
-
<tr><td>VF2</td><td>0.6&micro;L</td></tr>
 
-
<tr><td>VR</td><td>0.6&micro;L</td></tr>
 
-
<tr><td>MilliQ</td><td>13.8&micro;L</td></tr>
 
-
<tr><td>TOTAL</td><td>30&micro;L</td></tr>
 
-
</table>
 
-
 
-
<h2>8/2</h2>
 
-
<h3>Restriction Enzyme Digestion</h3>
 
-
<span class="author">Nakamura Matsumoto</span>
 
-
<table class="note">
 
-
<tr><th>Sample</th><th>Volume</th><th>EcoR1</th><th>Spe1</th><th>Buffer</th><th>MilliQ</th><th>TOTAL</tr>
 
-
<tr><td>8/2 dddD cloning</td><td>8.2</td><td>1</td><td>1</td><td>3</td><td>16.8</td><td>30</td></tr>
 
-
<tr><td>8/2 dddD cloning control</td><td>0.41</td><td>0</td><td>0</td><td>1</td><td>8.6</td><td>10</td></tr>
 
-
<tr><td>pSB1C3(control)</td><td>6.2</td><td>0</td><td>0</td><td>3(H)</td><td>2.8</td><td>10</td></tr>
 
-
</table>
 
-
 
-
<h2>8/3</h2>
 
-
<h3>Electrophoresis for confirmation</h3>
 
-
<span class="author">Murata</span>
 
-
<table class="note">
 
-
<tr><th>Lane</th><th>Sample</th></tr>
 
-
<tr><td>1</td><td>pSB1C3 dddD 1</td></tr>
 
-
<tr><td>2</td><td>pSB1C3 dddD 2</td></tr>
 
-
<tr><td>3</td><td>pSB1C3 dddD 3</td></tr>
 
-
<tr><td>4</td><td>1kb ladder(3&micro;L)</td></tr>
 
-
<tr><td>5</td><td>1kb ladder(1&micro;L)</td></tr>
 
-
</table>
 
-
 
-
<h2>8/3</h2>
 
-
<h3>Gel Extraction</h3>
 
-
<span class="author">Tatsui</span>
 
-
<table class="note">
 
-
<tr><th>Lane</th><th>DNA</th>
 
-
<tr><td>1</td><td>1kbp ladder</td></tr>
 
-
<tr><td>2</td><td>Blank</td></tr>
 
-
<tr><td>3</td><td>pSB1C3 25&micro;μL 2070bp</td></tr>
 
-
<tr><td>4</td><td>Blank</td></tr>
 
-
<tr><td>5</td><td>pSB1C3 5&micro;L</td></tr>
 
-
<tr><td>6</td><td>Blank</td></tr>
 
-
<tr><td>7</td><td>8/2 cloning dddD 25&micro;L 2544bp</td></tr>
 
-
<tr><td>8</td><td>Blank</td></tr>
 
-
<tr><td>9</td><td>3/24 PCR(dddD)cloning(control)</td></tr>
 
-
<tr><td>10</td><td>8/2 cloning dddD 5&micro;L</td></tr>
 
-
</table>
 
-
<h2>9/14</h2>
 
-
<h3>Gel Extraction</h3>
 
-
<span class="author">Honda,Yasuda</span>
 
-
<table class="note">
 
-
<tr><th>Lane</th><th>DNA</th>
 
-
<tr><td>1</td><td>1kbp ladder</td></tr>
 
-
<tr><td>2</td><td>3/24 PCR(dddD)cloning E,S</td></tr>
 
-
<tr><td>3</td><td>9/13SAMmT(control)</td></tr>
 
-
<tr><td>4</td><td>9/13SAMmT(EP)</td></tr>
 
-
<tr><td>5</td><td>9/13Pcon-pSB1C3(control)</td></tr>
 
-
<tr><td>6</td><td>9/13Pcon-pSB1C3(XS)</td></tr>
 
-
</table>
 
-
 
-
<h2>9/13</h2>
 
-
<h3>Column cleanup</h3>
 
-
<span class="author">Yasuda</span>
 
-
<table class="note">
 
-
<tr><th>Sample</th><th>density(&micro;g/mL)</td><td>260/280</td><td>260/230</th></tr>
 
-
<tr><td>9/12 AT-EP</td><td>2.3</td><td>1.33</td><td>1.11</td></tr>
 
-
<tr><td>9/12 AT-His-XP</td><td>2.0</td><td>1.33</td><td>0.86</td></tr>
 
-
</table>
 
-
 
-
<h2>9/13</h2>
 
-
<h3>Ligation</h3>
 
-
<span class="author">Honda</span>
 
-
<table class="note">
 
-
<tr><th>sumple</th><th>Vector</th><th>Insert</th><th>Ligase</th><th>2xBuffer</th><th>MilliQ</th></tr>
 
-
<tr><th>9/13 column cleanup AT(EP)+9/8 pSB1C3(EP)</th><td>1</td><td>3</td><td>1</td><td>5</td><td>0</td></tr>
 
-
<tr><th>9/13 column cleanup AT-His(XP)+9/13 pSB1C3(XP)</th><td>1</td><td>3</td><td>1</td><td>5</td><td>0</td></tr>
 
-
</table>
 
-
 
-
<h2>9/13</h2>
 
-
<h3>Miniprep</h3>
 
-
<span class="author">Honda</span>
 
-
<table class="note">
 
-
<tr><th>Sample</th><th>density(&micro;g/mL)</td><td>260/280</td><td>260/230</th></tr>
 
-
<tr><td>9/12 SAMmt-YCPlac111</td><td>140</td><td>1.80</td><td>1.90</td></tr>
 
-
<tr><td>9/12 T7-RBS-psB1C3</td><td>290</td><td>1.78</td><td>1.65</td></tr>
 
-
</table>
 
-
 
-
<h2>9/13</h2>
 
-
<h3>Liquid Culture</h3>
 
-
<span class="author">Yasuda</span>
 
-
<table class="note">
 
-
<tr><th>Sample</th><th>Medium</th></tr>
 
-
<tr><td>9/12 dddD-pSB1C3</td><td>CP</td></tr>
 
-
<tr><td>9/12 dddD-pSB1C3</td><td>CP</td></tr>
 
-
<tr><td>9/12 dddD-pSB1C3</td><td>CP</td></tr>
 
-
<tr><td>9/12 T7-RBS-His</td><td>CP</td></tr>
 
-
<tr><td>9/12 T7-RBS-His</td><td>CP</td></tr>
 
-
<tr><td>9/12 T7-RBS-His</td><td>CP</td></tr>
 
-
<tr><td>control</td><td>CP</td></tr>
 
-
</table>
 
-
 
-
<h2>9/14</h2>
 
-
<h3>Colony PCR</h3>
 
-
<span class="author">Yasuda</span>
 
-
<table class="note">
 
-
<tr><th></th><th>Master Mix</th></tr>
 
-
<tr><td>Quick Taq</td><td>335</td></tr>
 
-
<tr><td>VF</td><td>13.4</td></tr>
 
-
<tr><td>VR</td><td>13.4</td></tr>
 
-
<tr><td>MilliQ</td><td>308.2</td></tr>
 
-
<tr><td>TOTAL</td><td>670</td></tr>
 
-
</table>
 
-
 
-
<h2>9/14</h2>
 
-
<h3>Gel Extraction</h3>
 
-
<span class="author">Honda</span>
 
-
<table class="note">
 
-
<tr><th>Lane</th><th>DNA</th><th>volume</th>
 
-
<tr><td>1</td><td>1kbp ladder</td><td>5</td></tr>
 
-
<tr><td>2</td><td>3/24 PCR(dddD)cloning E,S</td><td>15</td></tr>
 
-
<tr><td>3</td><td>9/13SAMmT(control)</td><td>15</td></tr>
 
-
<tr><td>4</td><td>9/13SAMmT(EP)</td><td>15</td></tr>
 
-
<tr><td>5</td><td>9/13Pcon-pSB1C3(control)</td><td>15</td></tr>
 
-
<tr><td>6</td><td>9/13Pcon-pSB1C3(XS)</td></tr>
 
-
</table>
 
-
 
-
<h2>9/14</h2>
 
-
<h3>Electrophoresis for confirmation</h3>
 
-
<span class="author">Honda, Li and Yamauchi</span>
 
-
<table class="note">
 
-
<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</td></tr>
 
-
<tr><td>1</td><td>9/12 AT-YcPlac 1~8</td></tr>
 
-
<tr><td>2</td><td>9/12 AT-YcPlac 1~8</td></tr>
 
-
<tr><td>3</td><td>9/12 AT-YcPlac 1~8</td></tr>
 
-
<tr><td>4</td><td>9/12 AT-YcPlac 1~8</td></tr>
 
-
<tr><td>5</td><td>9/12 AT-YcPlac 1~8</td></tr>
 
-
<tr><td>6</td><td>9/12 AT-YcPlac 1~8</td></tr>
 
-
<tr><td>7</td><td>9/12 AT-YcPlac 1~8</td></tr>
 
-
<tr><td>8</td><td>9/12 AT-YcPlac 1~8</td></tr>
 
-
<tr><td>9</td><td>GeneRuler</td></tr>
 
-
<tr><td>10</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
 
-
<tr><td>11</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
 
-
<tr><td>12</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
 
-
<tr><td>13</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
 
-
<tr><td>14</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
 
-
<tr><td>15</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
 
-
<tr><td>16</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
 
-
<tr><td>17</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
 
-
</table>
 
-
<table class="note">
 
-
<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</td></tr>
 
-
<tr><td>1</td><td>9/12 mamB-YcPlac 1~8</td></tr>
 
-
<tr><td>2</td><td>9/12 mamB-YcPlac 1~8</td></tr>
 
-
<tr><td>3</td><td>9/12 mamB-YcPlac 1~8</td></tr>
 
-
<tr><td>4</td><td>9/12 mamB-YcPlac 1~8</td></tr>
 
-
<tr><td>5</td><td>9/12 mamB-YcPlac 1~8</td></tr>
 
-
<tr><td>6</td><td>9/12 mamB-YcPlac 1~8</td></tr>
 
-
<tr><td>7</td><td>9/12 mamB-YcPlac 1~8</td></tr>
 
-
<tr><td>8</td><td>9/12 mamB-YcPlac 1~8</td></tr>
 
-
<tr><td>9</td><td>GeneRuler</td></tr>
 
-
<tr><td>10</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
 
-
<tr><td>11</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
 
-
<tr><td>12</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
 
-
<tr><td>13</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
 
-
<tr><td>14</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
 
-
<tr><td>15</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
 
-
<tr><td>16</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
 
-
<tr><td>17</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
 
-
</table>
 
-
<table class="note">
 
-
<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</td></tr>
 
-
<tr><td>1</td><td>9/12 DECARB 9~16</td></tr>
 
-
<tr><td>2</td><td>9/12 DECARB 9~16</td></tr>
 
-
<tr><td>3</td><td>9/12 DECARB 9~16</td></tr>
 
-
<tr><td>4</td><td>9/12 DECARB 9~16</td></tr>
 
-
<tr><td>5</td><td>9/12 DECARB 9~16</td></tr>
 
-
<tr><td>6</td><td>9/12 DECARB 9~16</td></tr>
 
-
<tr><td>7</td><td>9/12 DECARB 9~16</td></tr>
 
-
<tr><td>8</td><td>9/12 DECARB 9~16</td></tr>
 
-
<tr><td>9</td><td>GeneRuler-YcPlac</td></tr>
 
-
<tr><td>10</td><td>9/12 DECARB-YcPlac 17~20</td></tr>
 
-
<tr><td>11</td><td>9/12 DECARB-Ycplac 17~20</td></tr>
 
-
<tr><td>12</td><td>9/12 DECARB-Ycplac 17~20</td></tr>
 
-
<tr><td>13</td><td>9/12 DiDECARB-YcPlac 1~4</td></tr>
 
-
<tr><td>14</td><td>9/12 DiDECARB-YcPlac 1~4</td></tr>
 
-
<tr><td>15</td><td>9/12 DiDECARB-YcPlac 1~4</td></tr>
 
-
<tr><td>16</td><td>9/12 DiDECARB-YcPlac 1~4</td></tr>
 
-
<tr><td>17</td><td>9/12 DiDECARB-YcPlac 1~4</td></tr>
 
-
</table>
 
-
<table class="note">
 
-
<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</td></tr>
 
-
<tr><td>1</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
 
-
<tr><td>2</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
 
-
<tr><td>3</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
 
-
<tr><td>4</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
 
-
<tr><td>5</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
 
-
<tr><td>6</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
 
-
<tr><td>7</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
 
-
<tr><td>8</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
 
-
<tr><td>9</td><td>GeneRuler</td></tr>
 
-
<tr><td>10</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
 
-
<tr><td>11</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
 
-
<tr><td>12</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
 
-
<tr><td>13</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
 
-
<tr><td>14</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
 
-
<tr><td>15</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
 
-
<tr><td>16</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
 
-
<tr><td>17</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
 
-
</table>
 
-
 
-
<h2>9/14</h2>
 
-
<h3>Miniprep</h3>
 
-
<span class="author">Honda</span>
 
-
<table class="note">
 
-
<tr><th>DNA</th><th>density(&micro;g/ml)</th><th>260/280</th><th>260/230</th></tr>
 
-
<tr><td>9/14 dddD(&micro;)-pSB1C3</td><td>645</td><td>1.82</td><td>1.70</td></tr>
 
-
<tr><td>9/14 dddD(&micro;)-pSB1C3</td><td>660</td><td>1.69</td><td>1.74</td></tr>
 
-
<tr><td>9/14 dddD(&micro;)-pSB1C3</td><td>635</td><td>1.87</td><td>1.57</td></tr>
 
-
<tr><td>9/14 T7-RBS-His-pSB1C3</td><td>440</td><td>1.65</td><td>1.23</td></tr>
 
-
<tr><td>9/14 T7-RBS-His-pSB1C3</td><td>450</td><td>1.92</td><td>1.46</td></tr>
 
-
<tr><td>9/14 T7-RBS-His-pSB1C3</td><td>190</td><td>1.ss</td><td>1.26</td></tr>
 
-
</table>
 
-
 
-
<h2>9/14</h2>
 
-
<h3>Restriction Enzyme Digestion</h3>
 
-
<span class="author">Honda</span>
 
-
<table class="note">
 
-
<tr><th>Sample</th><th>Volume</th><th>Spe1</th><th>Pst1</th><th>Xba1)</th><th>Buffur</th><th>BSA</th><th>MilliQ</th><th>TOTAL</tr>
 
-
<tr><td>9/14 dddD(&micro;)-pSB1C3</td><td>6</td><td>0</td><td>2</td><td>2</td><td>6(M)</td><td>6</td><td>38</td><td>60</td></tr>
 
-
<tr><td>9/14 dddD(&micro;)-pSB1C3</td><td>6</td><td>0</td><td>2</td><td>2</td><td>6(M)</td><td>6</td><td>38</td><td>60</td></tr>
 
-
<tr><td>9/14 dddD(&micro;)-pSB1C3</td><td>6</td><td>0</td><td>2</td><td>2</td><td>6(M)</td><td>6</td><td>38</td><td>60</td></tr>
 
-
<tr><td>9/14 T7-RBS-His-pSB1C3</td><td>5</td><td>1</td><td>1</td><td>0</td><td>3(H)</td><td>0</td><td>18</td><td>30</td></tr>
 
-
<tr><td>9/14 T7-RBS-His-pSB1C3</td><td>5</td><td>1</td><td>1</td><td>0</td><td>3(H)</td><td>0</td><td>18</td><td>30</td></tr>
 
-
<tr><td>9/14 T7-RBS-His-pSB1C3</td><td>10</td><td>1</td><td>1</td><td>0</td><td>3(H)</td><td>0</td><td>15</td><td>30</td></tr>
 
-
</table>
 
-
 
-
<h2>9/14</h2>
 
-
<h3>Ligation</h3>
 
-
<span class="author">Yasuda</span>
 
-
<table class="note">
 
-
<tr><th>NEB T4DNALigase</th><th>10XLig Buffer</th><th>9/12 DECARB XS column</th><th>9/14 Pcon-pSB1C3 XS Dephosphorylated</th><th>MilliQ</th><th>TOTAL</th></tr>
 
-
<tr><td>1</td><td>2</td><td>2.93</td><td>0.83</td><td>13.24</td><td>20</td></tr>
 
-
</table>
 
-
<table class="note">
 
-
<tr><th>NEB T4DNALigase</th><th>10XLig Buffer</th><th>9/12 DiDECARB XS column</th><th>9/14 Pcon-pSB1C3 XS Dephosphorylated</th><th>MilliQ</th><th>TOTAL</tr>
 
-
<tr><td>1</td><td>2</td><td>3.66</td><td>0.83</td><td>12.5</td><td>20</td></tr>
 
-
</table>
 
</table>
</table>
</div>
</div>
</html>
</html>

Revision as of 07:54, 23 September 2014

count down

edit

アメフラシノート

Retrieved from "http://2014.igem.org/Team:Kyoto/Notebook/DMS_Synthesis"