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Revision as of 14:20, 22 September 2014

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アメフラシノート

7/31

Restriction Enzyme Digestion

Nakamura Matsumoto

Sample	Volume	EcoR1	Spe1	Buffer(H)	MilliQ	TOTAL
3/24 PCR(dddD)cloning	3.2	1	1	3	21.8	30
pSB1C3	18.5	1	1	3	6.5	30
pSB1C3(control)	6.2	0	0	0	2.8	10

8/1

Gel Extraction

Yasuda Murata

Lane	DNA
1	1kbp ladder
2	3/24 PCR(dddD)cloning E,S
3	3/24 PCR(dddD)cloning E,S
4	Blank
5	pSB1C3
6	pSB1C3
7	Blank
8	1kbp ladder
9	3/24 PCR(dddD)cloning(control)
10	pSB1C3(control)

8/1

Ligation

Yasuda

Vector	inserter	10xt4LigaseBuffer	t4Ligase	MilliQ	TOTAL
1.5 µL(8/1 pSB1C3 2047bp 13.3µg/mL 20μg)	6.9 µL(8/1 dddD 2545bp 10.8µg/mL 74.60µg)	2µL	1µL	8.6µL	20µL

8/1

transformation

Murata

Name	Sample/(µl)	Competent Cells/(µl)	Total/(µl)	Plate
pSB1C3	3	30	165	SOC(132µl)

8/2

PCR Cloning

Murata

	dddD 121µg/µL
2x KAPA HiFiHotStartReadyMix	12.5µL
F Pre-dddD cloning 62	0.75µL
Rv Spe1-dddDcloning 68	0.75µL
MilliQ	11µL
TOTAL	25µL

8/2

Colony PCR

Honda Matsumoto Nakamura

	Master Mix
Quick Taq	15µL
VF2	0.6µL
VR	0.6µL
MilliQ	13.8µL
TOTAL	30µL

8/2

Restriction Enzyme Digestion

Nakamura Matsumoto

Sample	Volume	EcoR1	Spe1	Buffer	MilliQ	TOTAL
8/2 dddD cloning	8.2	1	1	3	16.8	30
8/2 dddD cloning control	0.41	0	0	1	8.6	10
pSB1C3(control)	6.2	0	0	3(H)	2.8	10

8/3

Electrophoresis for confirmation

Murata

Lane	Sample
1	pSB1C3 dddD 1
2	pSB1C3 dddD 2
3	pSB1C3 dddD 3
4	1kb ladder(3µL)
5	1kb ladder(1µL)

8/3

Gel Extraction

Tatsui

Lane	DNA
1	1kbp ladder
2	Blank
3	pSB1C3 25µμL 2070bp
4	Blank
5	pSB1C3 5µL
6	Blank
7	8/2 cloning dddD 25µL 2544bp
8	Blank
9	3/24 PCR(dddD)cloning(control)
10	8/2 cloning dddD 5µL

9/14

Gel Extraction

Honda,Yasuda

Lane	DNA
1	1kbp ladder
2	3/24 PCR(dddD)cloning E,S
3	9/13SAMmT(control)
4	9/13SAMmT(EP)
5	9/13Pcon-pSB1C3(control)
6	9/13Pcon-pSB1C3(XS)

9/13

Column cleanup

Yasuda

Sample	density(µg/mL)	260/280	260/230
9/12 AT-EP	2.3	1.33	1.11
9/12 AT-His-XP	2.0	1.33	0.86

9/13

Ligation

Honda

sumple	Vector	Insert	Ligase	2xBuffer	MilliQ
9/13 column cleanup AT(EP)+9/8 pSB1C3(EP)	1	3	1	5	0
9/13 column cleanup AT-His(XP)+9/13 pSB1C3(XP)	1	3	1	5	0

9/13

Miniprep

Honda

Sample	density(µg/mL)	260/280	260/230
9/12 SAMmt-YCPlac111	140	1.80	1.90
9/12 T7-RBS-psB1C3	290	1.78	1.65

9/13

Liquid Culture

Yasuda

Sample	Medium
9/12 dddD-pSB1C3	CP
9/12 dddD-pSB1C3	CP
9/12 dddD-pSB1C3	CP
9/12 T7-RBS-His	CP
9/12 T7-RBS-His	CP
9/12 T7-RBS-His	CP
control	CP

9/14

Colony PCR

Yasuda

	Master Mix
Quick Taq	335
VF	13.4
VR	13.4
MilliQ	308.2
TOTAL	670

9/14

Gel Extraction

Honda

Lane	DNA	volume
1	1kbp ladder	5
2	3/24 PCR(dddD)cloning E,S	15
3	9/13SAMmT(control)	15
4	9/13SAMmT(EP)	15
5	9/13Pcon-pSB1C3(control)	15
6	9/13Pcon-pSB1C3(XS)

9/14

Electrophoresis for confirmation

Honda, Li and Yamauchi

Lane	Restriction Enzyme Digestion Products
1	9/12 AT-YcPlac 1~8
2	9/12 AT-YcPlac 1~8
3	9/12 AT-YcPlac 1~8
4	9/12 AT-YcPlac 1~8
5	9/12 AT-YcPlac 1~8
6	9/12 AT-YcPlac 1~8
7	9/12 AT-YcPlac 1~8
8	9/12 AT-YcPlac 1~8
9	GeneRuler
10	9/12 AT-His-YcPlac 1~8
11	9/12 AT-His-YcPlac 1~8
12	9/12 AT-His-YcPlac 1~8
13	9/12 AT-His-YcPlac 1~8
14	9/12 AT-His-YcPlac 1~8
15	9/12 AT-His-YcPlac 1~8
16	9/12 AT-His-YcPlac 1~8
17	9/12 AT-His-YcPlac 1~8

Lane	Restriction Enzyme Digestion Products
1	9/12 mamB-YcPlac 1~8
2	9/12 mamB-YcPlac 1~8
3	9/12 mamB-YcPlac 1~8
4	9/12 mamB-YcPlac 1~8
5	9/12 mamB-YcPlac 1~8
6	9/12 mamB-YcPlac 1~8
7	9/12 mamB-YcPlac 1~8
8	9/12 mamB-YcPlac 1~8
9	GeneRuler
10	9/12 DECARB-YcPlac 1~8
11	9/12 DECARB-YcPlac 1~8
12	9/12 DECARB-YcPlac 1~8
13	9/12 DECARB-YcPlac 1~8
14	9/12 DECARB-YcPlac 1~8
15	9/12 DECARB-YcPlac 1~8
16	9/12 DECARB-YcPlac 1~8
17	9/12 DECARB-YcPlac 1~8

Lane	Restriction Enzyme Digestion Products
1	9/12 DECARB 9~16
2	9/12 DECARB 9~16
3	9/12 DECARB 9~16
4	9/12 DECARB 9~16
5	9/12 DECARB 9~16
6	9/12 DECARB 9~16
7	9/12 DECARB 9~16
8	9/12 DECARB 9~16
9	GeneRuler-YcPlac
10	9/12 DECARB-YcPlac 17~20
11	9/12 DECARB-Ycplac 17~20
12	9/12 DECARB-Ycplac 17~20
13	9/12 DiDECARB-YcPlac 1~4
14	9/12 DiDECARB-YcPlac 1~4
15	9/12 DiDECARB-YcPlac 1~4
16	9/12 DiDECARB-YcPlac 1~4
17	9/12 DiDECARB-YcPlac 1~4

Lane	Restriction Enzyme Digestion Products
1	9/12 DiDECARB-YcPlac 5~12
2	9/12 DiDECARB-YcPlac 5~12
3	9/12 DiDECARB-YcPlac 5~12
4	9/12 DiDECARB-YcPlac 5~12
5	9/12 DiDECARB-YcPlac 5~12
6	9/12 DiDECARB-YcPlac 5~12
7	9/12 DiDECARB-YcPlac 5~12
8	9/12 DiDECARB-YcPlac 5~12
9	GeneRuler
10	9/12 DiDECARB-YcPlac 13~20
11	9/12 DiDECARB-YcPlac 13~20
12	9/12 DiDECARB-YcPlac 13~20
13	9/12 DiDECARB-YcPlac 13~20
14	9/12 DiDECARB-YcPlac 13~20
15	9/12 DiDECARB-YcPlac 13~20
16	9/12 DiDECARB-YcPlac 13~20
17	9/12 DiDECARB-YcPlac 13~20

9/14

Miniprep

Honda

DNA	density(µg/ml)	260/280	260/230
9/14 dddD(µ)-pSB1C3	645	1.82	1.70
9/14 dddD(µ)-pSB1C3	660	1.69	1.74
9/14 dddD(µ)-pSB1C3	635	1.87	1.57
9/14 T7-RBS-His-pSB1C3	440	1.65	1.23
9/14 T7-RBS-His-pSB1C3	450	1.92	1.46
9/14 T7-RBS-His-pSB1C3	190	1.ss	1.26

9/14

Restriction Enzyme Digestion

Honda

Sample	Volume	Spe1	Pst1	Xba1)	Buffur	BSA	MilliQ	TOTAL
9/14 dddD(µ)-pSB1C3	6	0	2	2	6(M)	6	38	60
9/14 dddD(µ)-pSB1C3	6	0	2	2	6(M)	6	38	60
9/14 dddD(µ)-pSB1C3	6	0	2	2	6(M)	6	38	60
9/14 T7-RBS-His-pSB1C3	5	1	1	0	3(H)	0	18	30
9/14 T7-RBS-His-pSB1C3	5	1	1	0	3(H)	0	18	30
9/14 T7-RBS-His-pSB1C3	10	1	1	0	3(H)	0	15	30

9/14

Ligation

Yasuda

NEB T4DNALigase	10XLig Buffer	9/12 DECARB XS column	9/14 Pcon-pSB1C3 XS Dephosphorylated	MilliQ	TOTAL
1	2	2.93	0.83	13.24	20

NEB T4DNALigase	10XLig Buffer	9/12 DiDECARB XS column	9/14 Pcon-pSB1C3 XS Dephosphorylated	MilliQ	TOTAL
1	2	3.66	0.83	12.5	20

@@ Line 117: / Line 117: @@
 <tr><td>10</td><td>8/2 cloning dddD 5&micro;L</td></tr>
 </table>
-e7ba8b3a
+<h2>9/14</h2>
+<h3>Gel Extraction</h3>
+<span class="author">Honda,Yasuda</span>
+<table class="note">
+<tr><th>Lane</th><th>DNA</th>
+<tr><td>1</td><td>1kbp ladder</td></tr>
+<tr><td>2</td><td>3/24 PCR(dddD)cloning E,S</td></tr>
+<tr><td>3</td><td>9/13SAMmT(control)</td></tr>
+<tr><td>4</td><td>9/13SAMmT(EP)</td></tr>
+<tr><td>5</td><td>9/13Pcon-pSB1C3(control)</td></tr>
+<tr><td>6</td><td>9/13Pcon-pSB1C3(XS)</td></tr>
+</table>
+<h2>9/13</h2>
+<h3>Column cleanup</h3>
+<span class="author">Yasuda</span>
+<table class="note">
+<tr><th>Sample</th><th>density(&micro;g/mL)</td><td>260/280</td><td>260/230</th></tr>
+<tr><td>9/12 AT-EP</td><td>2.3</td><td>1.33</td><td>1.11</td></tr>
+<tr><td>9/12 AT-His-XP</td><td>2.0</td><td>1.33</td><td>0.86</td></tr>
+</table>
+<h2>9/13</h2>
+<h3>Ligation</h3>
+<span class="author">Honda</span>
+<table class="note">
+<tr><th>sumple</th><th>Vector</th><th>Insert</th><th>Ligase</th><th>2xBuffer</th><th>MilliQ</th></tr>
+<tr><th>9/13 column cleanup AT(EP)+9/8 pSB1C3(EP)</th><td>1</td><td>3</td><td>1</td><td>5</td><td>0</td></tr>
+<tr><th>9/13 column cleanup AT-His(XP)+9/13 pSB1C3(XP)</th><td>1</td><td>3</td><td>1</td><td>5</td><td>0</td></tr>
+</table>
+<h2>9/13</h2>
+<h3>Miniprep</h3>
+<span class="author">Honda</span>
+<table class="note">
+<tr><th>Sample</th><th>density(&micro;g/mL)</td><td>260/280</td><td>260/230</th></tr>
+<tr><td>9/12 SAMmt-YCPlac111</td><td>140</td><td>1.80</td><td>1.90</td></tr>
+<tr><td>9/12 T7-RBS-psB1C3</td><td>290</td><td>1.78</td><td>1.65</td></tr>
+</table>
+<h2>9/13</h2>
+<h3>Liquid Culture</h3>
+<span class="author">Yasuda</span>
+<table class="note">
+<tr><th>Sample</th><th>Medium</th></tr>
+<tr><td>9/12 dddD-pSB1C3</td><td>CP</td></tr>
+<tr><td>9/12 dddD-pSB1C3</td><td>CP</td></tr>
+<tr><td>9/12 dddD-pSB1C3</td><td>CP</td></tr>
+<tr><td>9/12 T7-RBS-His</td><td>CP</td></tr>
+<tr><td>9/12 T7-RBS-His</td><td>CP</td></tr>
+<tr><td>9/12 T7-RBS-His</td><td>CP</td></tr>
+<tr><td>control</td><td>CP</td></tr>
+</table>
+<h2>9/14</h2>
+<h3>Colony PCR</h3>
+<span class="author">Yasuda</span>
+<table class="note">
+<tr><th></th><th>Master Mix</th></tr>
+<tr><td>Quick Taq</td><td>335</td></tr>
+<tr><td>VF</td><td>13.4</td></tr>
+<tr><td>VR</td><td>13.4</td></tr>
+<tr><td>MilliQ</td><td>308.2</td></tr>
+<tr><td>TOTAL</td><td>670</td></tr>
+</table>
+<h2>9/14</h2>
+<h3>Gel Extraction</h3>
+<span class="author">Honda</span>
+<table class="note">
+<tr><th>Lane</th><th>DNA</th><th>volume</th>
+<tr><td>1</td><td>1kbp ladder</td><td>5</td></tr>
+<tr><td>2</td><td>3/24 PCR(dddD)cloning E,S</td><td>15</td></tr>
+<tr><td>3</td><td>9/13SAMmT(control)</td><td>15</td></tr>
+<tr><td>4</td><td>9/13SAMmT(EP)</td><td>15</td></tr>
+<tr><td>5</td><td>9/13Pcon-pSB1C3(control)</td><td>15</td></tr>
+<tr><td>6</td><td>9/13Pcon-pSB1C3(XS)</td></tr>
+</table>
+<h2>9/14</h2>
+<h3>Electrophoresis for confirmation</h3>
+<span class="author">Honda, Li and Yamauchi</span>
+<table class="note">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</td></tr>
+<tr><td>1</td><td>9/12 AT-YcPlac 1~8</td></tr>
+<tr><td>2</td><td>9/12 AT-YcPlac 1~8</td></tr>
+<tr><td>3</td><td>9/12 AT-YcPlac 1~8</td></tr>
+<tr><td>4</td><td>9/12 AT-YcPlac 1~8</td></tr>
+<tr><td>5</td><td>9/12 AT-YcPlac 1~8</td></tr>
+<tr><td>6</td><td>9/12 AT-YcPlac 1~8</td></tr>
+<tr><td>7</td><td>9/12 AT-YcPlac 1~8</td></tr>
+<tr><td>8</td><td>9/12 AT-YcPlac 1~8</td></tr>
+<tr><td>9</td><td>GeneRuler</td></tr>
+<tr><td>10</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
+<tr><td>11</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
+<tr><td>12</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
+<tr><td>13</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
+<tr><td>14</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
+<tr><td>15</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
+<tr><td>16</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
+<tr><td>17</td><td>9/12 AT-His-YcPlac 1~8</td></tr>
+</table>
+<table class="note">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</td></tr>
+<tr><td>1</td><td>9/12 mamB-YcPlac 1~8</td></tr>
+<tr><td>2</td><td>9/12 mamB-YcPlac 1~8</td></tr>
+<tr><td>3</td><td>9/12 mamB-YcPlac 1~8</td></tr>
+<tr><td>4</td><td>9/12 mamB-YcPlac 1~8</td></tr>
+<tr><td>5</td><td>9/12 mamB-YcPlac 1~8</td></tr>
+<tr><td>6</td><td>9/12 mamB-YcPlac 1~8</td></tr>
+<tr><td>7</td><td>9/12 mamB-YcPlac 1~8</td></tr>
+<tr><td>8</td><td>9/12 mamB-YcPlac 1~8</td></tr>
+<tr><td>9</td><td>GeneRuler</td></tr>
+<tr><td>10</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
+<tr><td>11</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
+<tr><td>12</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
+<tr><td>13</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
+<tr><td>14</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
+<tr><td>15</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
+<tr><td>16</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
+<tr><td>17</td><td>9/12 DECARB-YcPlac 1~8</td></tr>
+</table>
+<table class="note">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</td></tr>
+<tr><td>1</td><td>9/12 DECARB 9~16</td></tr>
+<tr><td>2</td><td>9/12 DECARB 9~16</td></tr>
+<tr><td>3</td><td>9/12 DECARB 9~16</td></tr>
+<tr><td>4</td><td>9/12 DECARB 9~16</td></tr>
+<tr><td>5</td><td>9/12 DECARB 9~16</td></tr>
+<tr><td>6</td><td>9/12 DECARB 9~16</td></tr>
+<tr><td>7</td><td>9/12 DECARB 9~16</td></tr>
+<tr><td>8</td><td>9/12 DECARB 9~16</td></tr>
+<tr><td>9</td><td>GeneRuler-YcPlac</td></tr>
+<tr><td>10</td><td>9/12 DECARB-YcPlac 17~20</td></tr>
+<tr><td>11</td><td>9/12 DECARB-Ycplac 17~20</td></tr>
+<tr><td>12</td><td>9/12 DECARB-Ycplac 17~20</td></tr>
+<tr><td>13</td><td>9/12 DiDECARB-YcPlac 1~4</td></tr>
+<tr><td>14</td><td>9/12 DiDECARB-YcPlac 1~4</td></tr>
+<tr><td>15</td><td>9/12 DiDECARB-YcPlac 1~4</td></tr>
+<tr><td>16</td><td>9/12 DiDECARB-YcPlac 1~4</td></tr>
+<tr><td>17</td><td>9/12 DiDECARB-YcPlac 1~4</td></tr>
+</table>
+<table class="note">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</td></tr>
+<tr><td>1</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
+<tr><td>2</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
+<tr><td>3</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
+<tr><td>4</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
+<tr><td>5</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
+<tr><td>6</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
+<tr><td>7</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
+<tr><td>8</td><td>9/12 DiDECARB-YcPlac 5~12</td></tr>
+<tr><td>9</td><td>GeneRuler</td></tr>
+<tr><td>10</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
+<tr><td>11</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
+<tr><td>12</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
+<tr><td>13</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
+<tr><td>14</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
+<tr><td>15</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
+<tr><td>16</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
+<tr><td>17</td><td>9/12 DiDECARB-YcPlac 13~20</td></tr>
+</table>
+<h2>9/14</h2>
+<h3>Miniprep</h3>
+<span class="author">Honda</span>
+<table class="note">
+<tr><th>DNA</th><th>density(&micro;g/ml)</th><th>260/280</th><th>260/230</th></tr>
+<tr><td>9/14 dddD(&micro;)-pSB1C3</td><td>645</td><td>1.82</td><td>1.70</td></tr>
+<tr><td>9/14 dddD(&micro;)-pSB1C3</td><td>660</td><td>1.69</td><td>1.74</td></tr>
+<tr><td>9/14 dddD(&micro;)-pSB1C3</td><td>635</td><td>1.87</td><td>1.57</td></tr>
+<tr><td>9/14 T7-RBS-His-pSB1C3</td><td>440</td><td>1.65</td><td>1.23</td></tr>
+<tr><td>9/14 T7-RBS-His-pSB1C3</td><td>450</td><td>1.92</td><td>1.46</td></tr>
+<tr><td>9/14 T7-RBS-His-pSB1C3</td><td>190</td><td>1.ss</td><td>1.26</td></tr>
+</table>
+<h2>9/14</h2>
+<h3>Restriction Enzyme Digestion</h3>
+<span class="author">Honda</span>
+<table class="note">
+<tr><th>Sample</th><th>Volume</th><th>Spe1</th><th>Pst1</th><th>Xba1)</th><th>Buffur</th><th>BSA</th><th>MilliQ</th><th>TOTAL</tr>
+<tr><td>9/14 dddD(&micro;)-pSB1C3</td><td>6</td><td>0</td><td>2</td><td>2</td><td>6(M)</td><td>6</td><td>38</td><td>60</td></tr>
+<tr><td>9/14 dddD(&micro;)-pSB1C3</td><td>6</td><td>0</td><td>2</td><td>2</td><td>6(M)</td><td>6</td><td>38</td><td>60</td></tr>
+<tr><td>9/14 dddD(&micro;)-pSB1C3</td><td>6</td><td>0</td><td>2</td><td>2</td><td>6(M)</td><td>6</td><td>38</td><td>60</td></tr>
+<tr><td>9/14 T7-RBS-His-pSB1C3</td><td>5</td><td>1</td><td>1</td><td>0</td><td>3(H)</td><td>0</td><td>18</td><td>30</td></tr>
+<tr><td>9/14 T7-RBS-His-pSB1C3</td><td>5</td><td>1</td><td>1</td><td>0</td><td>3(H)</td><td>0</td><td>18</td><td>30</td></tr>
+<tr><td>9/14 T7-RBS-His-pSB1C3</td><td>10</td><td>1</td><td>1</td><td>0</td><td>3(H)</td><td>0</td><td>15</td><td>30</td></tr>
+</table>
+<h2>9/14</h2>
+<h3>Ligation</h3>
+<span class="author">Yasuda</span>
+<table class="note">
+<tr><th>NEB T4DNALigase</th><th>10XLig Buffer</th><th>9/12 DECARB XS column</th><th>9/14 Pcon-pSB1C3 XS Dephosphorylated</th><th>MilliQ</th><th>TOTAL</th></tr>
+<tr><td>1</td><td>2</td><td>2.93</td><td>0.83</td><td>13.24</td><td>20</td></tr>
+</table>
+<table class="note">
+<tr><th>NEB T4DNALigase</th><th>10XLig Buffer</th><th>9/12 DiDECARB XS column</th><th>9/14 Pcon-pSB1C3 XS Dephosphorylated</th><th>MilliQ</th><th>TOTAL</tr>
+<tr><td>1</td><td>2</td><td>3.66</td><td>0.83</td><td>12.5</td><td>20</td></tr>
+</table>
 </table>
 </div>
 </html>

Team:Kyoto/Notebook/DMS Synthesis

From 2014.igem.org

Revision as of 14:20, 22 September 2014

アメフラシノート

7/31

Restriction Enzyme Digestion

8/1

Gel Extraction

8/1

Ligation

8/1

transformation

8/2

PCR Cloning

8/2

Colony PCR

8/2

Restriction Enzyme Digestion

8/3

Electrophoresis for confirmation

8/3

Gel Extraction

9/14

Gel Extraction

9/13

Column cleanup

9/13

Ligation

9/13

Miniprep

9/13

Liquid Culture

9/14

Colony PCR

9/14

Gel Extraction

9/14

Electrophoresis for confirmation

9/14

Miniprep

9/14

Restriction Enzyme Digestion

9/14

Ligation