Team:CSU Fort Collins

From 2014.igem.org

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<ul>
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   <li class='active'><a href='/Team:CSU_Fort_Collins'><span>Home</span></a></li>
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   <li><a href='/Team:CSU_Fort_Collins'><span>Home</span></a></li>
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Project/'><span>Project</span></a>
   <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Project/'><span>Project</span></a>
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   <li class='has-sub'><a href='Team:CSU_Fort_Collins/Notebook/'><span>Notebook</span></a>
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   <li class='has-sub' class='active'><a href='Team:CSU_Fort_Collins/Notebook/'><span>Notebook</span></a>
     <ul style="display:block">
     <ul style="display:block">
       <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Protocols'><span>Protocols</span></a>
       <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Protocols'><span>Protocols</span></a>
         <ul>
         <ul>
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           <li class='last'><a href="#"><span>Protocol 1</span></a></li>
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          <li><a href="/Team:CSU_Fort_Collins/Notebook/Protocols=Gibson"><span>Gibson Assembly</span></a></li>
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          <li><a href="/Team:CSU_Fort_Collins/Notebook/Protocols=Cloning"><span>Cloning Genes</span></a></li>
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          <li><a href="/Team:CSU_Fort_Collins/Notebook/Protocols=Miniprep"><span>Plasmid Miniprep</span></a></li>
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          <li><a href="/Team:CSU_Fort_Collins/Notebook/Protocols=Isolation"><span>Yeast DNA Isolation</span></a></li>
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          <li><a href="/Team:CSU_Fort_Collins/Notebook/Protocols=Gel"><span>Gel Electrophoresis</span></a></li>
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           <li class='last'><a href="/Team:CSU_Fort_Collins/Notebook/Protocols=Purify"><span>PCR Product Purification</span></a></li>
         </ul>
         </ul>
       </li>
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         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/'><span>Breakdown</span></a>
         <li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/Breakdown/'><span>Breakdown</span></a>
             <ul>
             <ul>
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              <li><a href="#"><span>June</span></a></li>
 
               <li><a href="#"><span>July</span></a></li>
               <li><a href="#"><span>July</span></a></li>
               <li><a href='#'><span>August</span></a></li>
               <li><a href='#'><span>August</span></a></li>
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         <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/'><span>Kill Switch</span></a>
         <li li class='has-sub'><a href='/Team:CSU_Fort_Collins/Notebook/KillSwitch/'><span>Kill Switch</span></a>
             <ul>
             <ul>
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              <li><a href="#"><span>June</span></a></li>
 
               <li><a href="#"><span>July</span></a></li>
               <li><a href="#"><span>July</span></a></li>
               <li><a href='#'><span>August</span></a></li>
               <li><a href='#'><span>August</span></a></li>
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   </li>
   </li>
   <li><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li>
   <li><a href='/Team:CSU_Fort_Collins/Safety/'><span>Safety</span></a></li>
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  <li class='last'><a href='https://2014.igem.org/Main_Page'><img src='http://oi62.tinypic.com/11rxaxj.jpg' class='logo'/></a></li>
 
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    <h1>Our Team</h1>
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    <h1>Welcome to CSU Fort Collin's 2014 Wiki</h1>
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    <div class="col" data-name="adriana-collings">
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          Adriana Collings<br>
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      <h3>Our Project</h3>
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          <a href="mailto:adriana.collings@rams.colostate.edu"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
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          Anthony Roulier<br>
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          <a href="mailto:aroulier@rams.colostate.edu"><img src="https://static.igem.org/mediawiki/2014/thumb/1/13/CSU_EmailC.png/600px-CSU_EmailC.png" class="icon"/></a>
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          Chauncy Hinshaw<br>
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      Approximately 3 billion gallons per year of used frying oil are produced in the U.S. alone. While some recycling efforts are put forth to turn used frying oil into biodiesel and some companies are working on more effective ways to recycle the frying oil, the iGEM team at Colorado State University is working to turn spent frying oil into a high value product. There are four major components to this year’s project including; the breakdown of frying oil, a biosensor for detecting the breakdown of frying oil, production of a high value product, and a kill switch to kill the bacteria if they were to be released into the environment. Our team aims to put each of these components into Escherichia coli. 
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<br><br>
 +
In order to detect that the process is working we have developed a biosensor that will detect the breakdown of Acyl-CoA, a byproduct of the breakdown pathway of lipids, in E. coli. Our biosensor is being constructed by inserting a novel promoter in front of DNA expressing a Green Fluorescent Protein (GFP). This promoter is activated by Acyl-CoA, a byproduct of the breakdown pathway of lipids in E. coli. This will result in E. coli expressing GFP when successful breakdown is occurring.
 +
<br><br>
 +
In order to break down the frying oil we will be taking advantage of the cell’s natural ability to break down fatty acids for use in the Kreb’s cycle. We will be upregulating the limiting enzymes that aid in the breakdown of fatty acids in order to have the cell produce more Acetyl-CoA.
 +
<br><br>
 +
This Acetyl-CoA can then be used in the mevalonate pathway. This pathway is common to most plants as well as yeast. We will be taking the genes from yeast and constructing two operons in order to produce isopentenyl pyrophosphate (IPP). While both IPP and dimethylallyl pyrophosphate (DMAPP) are both naturally produced in E. coli  through the non-mevalonate pathway , the mevalonate pathway provides a more efficient route from acetyl-CoA to terpenoid production than the alternative. Our high-value product will be a terpenoid that has yet to be determined.
 +
<br><br>
 +
For our kill switch, we will be using a gene named Killer Red which is a mutant of hydrozoan chromoprotein anm2CP from Anthomedusae sp. DC-2005 . Our team plans to insert this gene into our construct and by doing so, it will provide a fail-safe in the unlikely event of our E. coli escaping into the environment. The Killer Red gene has a repressible promoter in which tryptophan acts as the repressor. There will be tryptophan present while the cell is used to break down the frying oil, but is not highly present in the environment. Without the presence of tryptophan the Killer Red gene will be activated and white light will produce reactive oxygen species within the cell, causing cell death.
 +
<br><br>
 +
Our biosensor is being constructed by inserting a novel promoter in front of DNA expressing a Green Fluorescent Protein (GFP). This promoter is activated by Acyl-CoA, a byproduct of the breakdown pathway of lipids in E. coli. This will result in E. coli expressing GFP when successful breakdown is occurring.
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    <div class="bio" id="adriana-collings">
 
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          <b>Major:</b> Microbiology<br>
 
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          <b>Semester:</b> 4<br>
 
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          <b>iGEM Topic:</b> High-Value Product<br>
 
-
          <b>Other Positions:</b> Lab Assistant (Tuberculosis research), President, Synthetic Biology Club<br>
 
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          <b>Interests:</b> Molecular Biology, Immunology, Bacteriology<br>
 
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    </div>
 
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    <div class="bio" id="anthony-roulier">
 
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          <b>Major:</b> Chemical/Biological Engineering<br>
 
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          <b>Semester:</b> 4<br>
 
-
          <b>iGEM Topic:</b> Breakdown<br>
 
-
          <b>Other Positions:</b> Member, AICHE<br>
 
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          <b>Interests:</b> Fermentation, Food Microbiology<br>
 
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    <div class="bio" id="chauncy-hinshaw">
 
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          <b>Major:</b> Microbiology<br>
 
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          <b>Semester:</b> 4<br>
 
-
          <b>iGEM Topic:</b> Breakdown<br>
 
-
          <b>Other Positions:</b> Lab Assistant (Tuberculosis research); President, Microbiology Student Association; Treasurer, College of Veterinary
 
-
          Medicine and Biosciences College Council<br>
 
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          <b>Interests:</b> Epidemiology, Public Health Education, Gene Therapy<br>
 
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          Krista Henderson<br>
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          Matt Sabel<br>
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          <b>Major:</b> Biochemistry<br>
 
-
          <b>Semester:</b> 6<br>
 
-
          <b>iGEM Topic:</b> Biosensor<br>
 
-
          <b>Other Positions:</b> Lab Assistant (Tomato mosaic virus research)<br>
 
-
          <b>Interests:</b> Epigenetics, Manufacturing<br>
 
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    </div>
 
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    <div class="bio" id="krista-henderson">
 
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          <b>Major:</b> Chemical/Biological Engineering<br>
 
-
          <b>Semester:</b> 4<br>
 
-
          <b>iGEM Topic:</b> Breakdown<br>
 
-
          <b>Other Positions:</b> Member, CSU Marching Band<br>
 
-
          <b>Interests:</b> Scientific Literacy, Gene Therapy<br>   
 
-
    </div>
 
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          <b>Major:</b> Microbiology<br>
 
-
          <b>Semester:</b> 2<br>
 
-
          <b>iGEM Topic:</b> Kill Switch<br>
 
-
          <b>Other Positions:</b> IT (CSU Veterinary Teaching Hospital)<br>
 
-
          <b>Interests:</b> Virology, Pathology<br></td>
 
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        Renee Plomondon<br>
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          Savannah Roemer<br>
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           <b>Major:</b> Chemical/Biological Engineering, Biomedical Engineering<br>
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          <b>Semester:</b> 4<br>
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          <b>iGEM Topic:</b> High-Value Product<br>
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          <b>Other Positions:</b> Lab Assistant (Hepatocyte research); Underwriting Director, DJ (KCSU 90.5 FM)<br>
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          <b>Interests:</b> Tissue Engineering, Web/Graphic Design, Programming<br>
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          <b>Major:</b> Biochemistry<br>
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          <b>Semester:</b> 2<br>
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          <b>iGEM Topic:</b> Kill Switch<br>
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          <b>Other Positions:</b> Lab Assistant (Musculoskeletal Biomechanics research)<br>
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          <b>Interests:</b> Bionics, Biomedicine<br>
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    <div class="bio" id="savannah-roemer">
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          <b>Major:</b> Chemical/Biological Engineering<br>
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          <b>Semester:</b> 4<br>
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-
          <b>Other Positions:</b> Lab Assistant (Tuberculosis research), International Field Biology Research Assistant (Euglossine bees, entomology research)<br>
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          <b>Interests:</b> Biochemistry, Genetics, Horticulture<br>
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          Sun Jiayi<br>
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          Ashok Prasad, Ph.D.<br>
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          Christie Peebles, Ph.D.<br>
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          <b>Department:</b> Chemical/Biological Engineering<br>
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          <b>Research Interests:</b> Sun is a graduate student at CSU working in Dr. Peebles lab. Specifically, she works with periwinkles. She advises all of the iGEM team members in day-to-day activities.<br><br>
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          <b>Department:</b> Chemical/Biological Engineering<br>
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          <b>Research Interests:</b> Dr. Prasad's research is driven by concrete biological and bio-engineering questions as well as the larger questions posed by systems biology and quantitative biology. He investigates questions such as 'What does it mean to say that life is an emergent property of complex biological networks? Are there non-trivial universal principles that "living matter" obeys?' The main method his lab uses to answer these questions is creation and development of predictive biological models.<br><br>
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          <b>Research Interests:</b> Dr. Peebles' research is focused in the areas of metabolic engineering, secondary metabolism, regulatory networks, and systems biology in plants, bacteria and yeast for the production of bio-based chemicals and fuels. More specifically, these areas can be divided into 2 main applications. One application is the use of plant metabolic engineering to produce important pharmaceuticals and nutraceuticals. The second is the engineering of photoautotrophs for the production of bio-based chemicals and fuels.<br><br>
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Revision as of 02:57, 22 September 2014

CSU iGEM 2014

Welcome to CSU Fort Collin's 2014 Wiki

Our Project

Approximately 3 billion gallons per year of used frying oil are produced in the U.S. alone. While some recycling efforts are put forth to turn used frying oil into biodiesel and some companies are working on more effective ways to recycle the frying oil, the iGEM team at Colorado State University is working to turn spent frying oil into a high value product. There are four major components to this year’s project including; the breakdown of frying oil, a biosensor for detecting the breakdown of frying oil, production of a high value product, and a kill switch to kill the bacteria if they were to be released into the environment. Our team aims to put each of these components into Escherichia coli.

In order to detect that the process is working we have developed a biosensor that will detect the breakdown of Acyl-CoA, a byproduct of the breakdown pathway of lipids, in E. coli. Our biosensor is being constructed by inserting a novel promoter in front of DNA expressing a Green Fluorescent Protein (GFP). This promoter is activated by Acyl-CoA, a byproduct of the breakdown pathway of lipids in E. coli. This will result in E. coli expressing GFP when successful breakdown is occurring.

In order to break down the frying oil we will be taking advantage of the cell’s natural ability to break down fatty acids for use in the Kreb’s cycle. We will be upregulating the limiting enzymes that aid in the breakdown of fatty acids in order to have the cell produce more Acetyl-CoA.

This Acetyl-CoA can then be used in the mevalonate pathway. This pathway is common to most plants as well as yeast. We will be taking the genes from yeast and constructing two operons in order to produce isopentenyl pyrophosphate (IPP). While both IPP and dimethylallyl pyrophosphate (DMAPP) are both naturally produced in E. coli through the non-mevalonate pathway , the mevalonate pathway provides a more efficient route from acetyl-CoA to terpenoid production than the alternative. Our high-value product will be a terpenoid that has yet to be determined.

For our kill switch, we will be using a gene named Killer Red which is a mutant of hydrozoan chromoprotein anm2CP from Anthomedusae sp. DC-2005 . Our team plans to insert this gene into our construct and by doing so, it will provide a fail-safe in the unlikely event of our E. coli escaping into the environment. The Killer Red gene has a repressible promoter in which tryptophan acts as the repressor. There will be tryptophan present while the cell is used to break down the frying oil, but is not highly present in the environment. Without the presence of tryptophan the Killer Red gene will be activated and white light will produce reactive oxygen species within the cell, causing cell death.

Our biosensor is being constructed by inserting a novel promoter in front of DNA expressing a Green Fluorescent Protein (GFP). This promoter is activated by Acyl-CoA, a byproduct of the breakdown pathway of lipids in E. coli. This will result in E. coli expressing GFP when successful breakdown is occurring.

Retrieved from "http://2014.igem.org/Team:CSU_Fort_Collins"