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- Intro
- Problem
- Situation
- Idea
- Solution
- Platform
Our Project
Chlorophyll is a core component in the process of photosynthesis. As a pigment,
it harvests light and plays a primary role in the excitation transfer of energy
(Eichwurzel, Stiel et al. 2000), which is vital for plant reproduction and survival
(Uliana, Pires et al. 2014). The chlorophyll biochemical pathway is an oxygenic
photosynthetic process that oxidizes water to produce hydrogen ions. Thirteen
genes govern the five-step pathway and each has a specific role.
The 2014 Macquarie University iGEM team is continuing the work of the 2013 team
to synthetically construct the chlorophyll biochemical pathway in E.coli using
synthetic Biobricks from Chlamydomonas reinhardtii. The Biobricks from 2013 have
been made using Gibson Assembly. Our aim for 2014 is to improve the Biobricks using
synthetic techniques, which will be assembled into three functional operons and
expressed in E.coli competent cells.
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