Team:ULB-Brussels

From 2014.igem.org

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<section style="text-align: justify; margin: 25px"><p>$\Tgo$his year, the Université Libre de Bruxelles competes again in the International Genetically Engineered Machines contest, a competition of synthetic biology organized by the Massachussetts Institute of Technology. Year 2014 will mark the 10th anniversary of the IGEM and will feature a "Giant Jamboree", where all teams will be invited in Boston without regional pre-selection.</p>
<section style="text-align: justify; margin: 25px"><p>$\Tgo$his year, the Université Libre de Bruxelles competes again in the International Genetically Engineered Machines contest, a competition of synthetic biology organized by the Massachussetts Institute of Technology. Year 2014 will mark the 10th anniversary of the IGEM and will feature a "Giant Jamboree", where all teams will be invited in Boston without regional pre-selection.</p>
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<p>Our aim is <i>to enhance recombinant protein production in bioreactors by addicting microorganisms to the production of a $\small \underline{p}rotein$ $\small of$ $\small \underline{i}nterest$</i>. We built $\MyColi$, a recombinant $\small Escherichia$ $\small Coli$ expressing a toxin and a $\PI$. This protein will be on the same open reading frame as an antoxin, a protein able to neutralize the effects of the toxin. Stressed subpopulations might not express the $\PI$ (and thus the antitoxin) to a sufficient level, allowing the toxin to kill unproductive cells. Our work will consist on building and modelling this system throughout the summer. </p></section>
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<p>Our aim is <i>to enhance recombinant protein production in bioreactors by addicting microorganisms to the production of a $\small protein$ $\small of$ $\small interest, \PI$</i>.  
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We built $\underline{\MyColi}$, a recombinant $\small Escherichia$ $\small Coli$ expressing a toxin and a $\PI$. This protein will be on the same open reading frame as an antoxin, a protein able to neutralize the effects of the toxin. Stressed subpopulations might not express the $\PI$ (and thus the antitoxin) to a sufficient level, allowing the toxin to kill unproductive cells. Our work will consist on building and modelling this system throughout the summer. </p></section>
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Revision as of 15:00, 20 September 2014

$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$ Example of a hierarchical menu in CSS

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- Université Libre de Bruxelles -



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$\Tgo$his year, the Université Libre de Bruxelles competes again in the International Genetically Engineered Machines contest, a competition of synthetic biology organized by the Massachussetts Institute of Technology. Year 2014 will mark the 10th anniversary of the IGEM and will feature a "Giant Jamboree", where all teams will be invited in Boston without regional pre-selection.

Our aim is to enhance recombinant protein production in bioreactors by addicting microorganisms to the production of a $\small protein$ $\small of$ $\small interest, \PI$. We built $\underline{\MyColi}$, a recombinant $\small Escherichia$ $\small Coli$ expressing a toxin and a $\PI$. This protein will be on the same open reading frame as an antoxin, a protein able to neutralize the effects of the toxin. Stressed subpopulations might not express the $\PI$ (and thus the antitoxin) to a sufficient level, allowing the toxin to kill unproductive cells. Our work will consist on building and modelling this system throughout the summer.



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