Team:ULB-Brussels/Modelling/2A-Peptid

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Revision as of 12:43, 20 September 2014

$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$ Example of a hierarchical menu in CSS

-Du nouveau pour comparer avec et sans p2A? Yes, now we get it!

- Université Libre de Bruxelles -

2A Peptide

Now we're ready to compare our $\MyColi$ with the usual $\Stabi$ system.

The objective of $\Stabi$ is to stabilize the presence of the plasmids that contain the $\PI$ gene sequence. $E.Coli$ can insert other plasmids, containing the $Tox$ and the $A-Tox$ genes. These two proteins are produced at same rate (contitutive promoter: T7) and the $\PI$ is independently produced in this classical system.

The objective of $\Stabi$ is a little bit different: The T7 promoter iniciates the transcription of the $\PI$ $\&$ the $A-Tox$ genes, but another promoter, pBAD, controls the transcription of the $Tox$ gene, situated in another plasmid than the $\PI$ $\&$ the $A-Tox$ genes.

The problem with the classical system is that if there are a lot of mutations or a lot of heterogeneity in the E.Coli population, the $PI$ is not produced at high rate. In a bioreactor built to product a special kind of $\PI$, this system's not optimal because there're $\PI$ losses, especially if the $\PI$ gene sequence is long or if the $\PI$ is cumbersome for $E.Coli$.

< TA System

Conclusion >

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-<!-- ! $2 A$ $\small peptide$ $\small Modelling$ $\small page.$
+<!-- ! $2 A$ $\small peptide$ $\small Modelling$ $\small page.$ -->
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-$\newcommand{\Stabi}{\small Stabi \normalsize}$
 <section style="text-align: right; margin: 50px">
 <p> Now we're ready to compare our $\MyColi$ with the usual $\Stabi$ system.</p>
-</section>
+The objective of $\Stabi$ is to stabilize the presence of the plasmids that contain the $\PI$ gene sequence.
+$E.Coli$ can insert other plasmids, containing the $Tox$ and the $A-Tox$ genes.
+These two proteins are produced at same rate (contitutive promoter: T7) and the $\PI$ is independently produced in this classical system.</p>
+The objective of $\Stabi$ is a little bit different: The T7 promoter iniciates the transcription of the $\PI$ $\&$ the $A-Tox$ genes, but another promoter, pBAD, controls the transcription of the $Tox$ gene, situated in another plasmid than the $\PI$ $\&$ the $A-Tox$ genes. </p>
+The problem with the classical system is that if there are a lot of mutations or a lot of heterogeneity in the E.Coli population, the $PI$ is not produced at high rate. In a bioreactor built to product a special kind of $\PI$, this system's not optimal because there're $\PI$ losses, especially if the $\PI$ gene sequence is long or if the $\PI$ is cumbersome for $E.Coli$.
 </tr>