Team:Evry/Interlab Study/09-18-2014

From 2014.igem.org

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<li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
<li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h-16h).
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<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h43-23h43).
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<img src="" alt="PLAN DE PLAQUE" />
<img src="" alt="PLAN DE PLAQUE" />

Revision as of 20:16, 19 September 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010


Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).
PLAN DE PLAQUE


Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23101:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h43-23h43).
PLAN DE PLAQUE

PSB1C3+E0240+J23102:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23104:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23105:
glycerol stock preparation from LB culture of the 17th September.

PSB1C3+E0240+J23107:
glycerol stock preparation from LB culture of the 17th September.

PSB1C3+E0240+J23110:
Pre-culture does'nt grow. The TECAN measure is reported.

PSB1C3+E0240+J23115:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23116:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23117:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
Sep 18