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Team:Evry/Interlab Study/08-22-2014
From 2014.igem.org
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<br/>- BBa_K823012: 100-150 isolated colonies | <br/>- BBa_K823012: 100-150 isolated colonies | ||
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| - | <br/> A PCR was performed | + | <br/> A PCR was performed with 8 colonies for BBa_K823012 and for BBa_J23101 following the protocol Table 3 and 4. |
<br/> To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X. | <br/> To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X. | ||
Latest revision as of 17:59, 19 September 2014
Contructions PSB1C3 with I20260, J23101-E1010 and K823012-E1010
Transformation plate observation:
- BBa_J23101: 11 isolated colonies
- BBa_K823012: 100-150 isolated colonies
A PCR was performed with 8 colonies for BBa_K823012 and for BBa_J23101 following the protocol Table 3 and 4.
To verify PCR products, 10 µl was loaded on a 1% agarose gel with 2 µl of loading dye 6X.
We expected to obtain a band between 300 and 400 bp. So we decided to amplify the colony n°8. The colony was seed in 5 ml of LB medium and 5 µl of chloramphenicol. Culture was incubated overnight at 37°C with 200 rpm agitation.
We expected to obtain a band around 1200 bp. So we decided to amplify the colony n°2 corresponding to lane 11. The colony was seed in 5 ml of LB medium and 5 µl of chloramphenicol. Culture was incubated overnight at 37°C with 200 rpm agitation. Aug 22
